Recombinant Human PRP19 protein - BSA and Azide free is a Human Full Length protein, in the 2 to 504 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
S L I C S I S N E V P E H P C V S P V S N H V Y E R R L I E K Y I A E N G T D P I N N Q P L S E E Q L I D I K V A H P I R P K P P S A T S I P A I L K A L Q D E W D A V M L H S F T L R Q Q L Q T T R Q E L S H A L Y Q H D A A C R V I A R L T K E V T A A R E A L A T L K P Q A G L I V P Q A V P S S Q P S V V G A G E P M D L G E L V G M T P E I I Q K L Q D K A T V L T T E R K K R G K T V P E E L V K P E E L S K Y R Q V A S H V G L H S A S I P G I L A L D L C P S D T N K I L T G G A D K N V V V F D K S S E Q I L A T L K G H T K K V T S V V F H P S Q D L V F S A S P D A T I R I W S V P N A S C V Q V V R A H E S A V T G L S L H A T G D Y L L S S S D D Q Y W A F S D I Q T G R V L T K V T D E T S G C S L T C A Q F H P D G L I F G T G T M D S Q I K I W D L K E R T N V A N F P G H S G P I T S I A F S E N G Y Y L A T A A D D S S V K L W D L R K L K N F K T L Q L D N N F E V K S L I F D Q S G T Y L A L G G T D V Q I Y I C K Q W T E I L H F T E H S G L T T G V A F G H H A K F I A S T G M D R S L K F Y S L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome (PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:30705154). Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).
Alternative names
NMP200, PRP19, SNEV, PRPF19, Pre-mRNA-processing factor 19, Nuclear matrix protein 200, PRP19/PSO4 homolog, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, hPso4
Recommended products
Recombinant Human PRP19 protein - BSA and Azide free is a Human Full Length protein, in the 2 to 504 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q9UMS4-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- S L I C S I S N E V P E H P C V S P V S N H V Y E R R L I E K Y I A E N G T D P I N N Q P L S E E Q L I D I K V A H P I R P K P P S A T S I P A I L K A L Q D E W D A V M L H S F T L R Q Q L Q T T R Q E L S H A L Y Q H D A A C R V I A R L T K E V T A A R E A L A T L K P Q A G L I V P Q A V P S S Q P S V V G A G E P M D L G E L V G M T P E I I Q K L Q D K A T V L T T E R K K R G K T V P E E L V K P E E L S K Y R Q V A S H V G L H S A S I P G I L A L D L C P S D T N K I L T G G A D K N V V V F D K S S E Q I L A T L K G H T K K V T S V V F H P S Q D L V F S A S P D A T I R I W S V P N A S C V Q V V R A H E S A V T G L S L H A T G D Y L L S S S D D Q Y W A F S D I Q T G R V L T K V T D E T S G C S L T C A Q F H P D G L I F G T G T M D S Q I K I W D L K E R T N V A N F P G H S G P I T S I A F S E N G Y Y L A T A A D D S S V K L W D L R K L K N F K T L Q L D N N F E V K S L I F D Q S G T Y L A L G G T D V Q I Y I C K Q W T E I L H F T E H S G L T T G V A F G H H A K F I A S T G M D R S L K F Y S L
- Accession
- Q9UMS4
- Protein length
- Full Length
- Predicted molecular weight
- 55 kDa
- Amino acids
- 2 to 504
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome (PubMed:28076346, PubMed:28502770, PubMed:29301961, PubMed:29360106, PubMed:30705154). Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity).
- Sequence similarities
Belongs to the WD repeat PRP19 family.
- Subcellular localisation
- Nucleus, Nucleoplasm, Cytoskeleton, Spindle
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PRP19 protein also known as Pre-mRNA Processing factor 19 weighs approximately 55 kDa. This protein plays a role in the splicing of pre-mRNA by functioning as part of the spliceosome complex. PRP19 is expressed in many tissues and cells which reflects its important role in RNA processing. It is not confined to a specific type of tissue indicating its broad relevance in cellular processes. Mechanically PRP19 acts by enhancing the efficiency of splicing ensuring that mRNA molecules are properly matured for translation.
- Biological function summary
PRP19 engages in multiple processes beyond its initial splicing role. It forms part of the NTC complex which is critical for the activation of the spliceosome. This complex includes other key proteins essential for RNA splicing transition phases. Additionally PRP19 contributes to DNA repair mechanisms playing a part in maintaining genomic integrity under stress conditions. Its role in these processes demonstrates its versatile functionality in cell homeostasis and response to damage.
- Pathways
Experts recognize PRP19's involvement in the mRNA processing and DNA damage response pathways. These pathways are key to understanding how cells manage genetic information and react to physiological challenges. PRP19 interacts with other proteins such as CDC5L and PLRG1 within these pathways illustrating its interconnected role in cellular regulation. These associations underline how PRP19 integrates into critical networks that balance gene expression and genome stability.
- Associated diseases and disorders
Researchers have linked PRP19 to cancer and neurodegenerative disorders. In cancer abnormal PRP19 function may contribute to unchecked cell growth by impacting mRNA splicing and DNA repair. In neurodegenerative diseases its dysfunction might influence neuron survival through defective RNA processing. The relationship of PRP19 with proteins like BRCA1 in the context of cancer further highlights its significance in disease mechanisms. As such PRP19 serves as a potential target for therapeutic exploration.
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1 product image
SDS-PAGE - Recombinant Human PRP19 protein - BSA and Azide free (ab172187)
SDS-PAGE showing ab172187. Approx. MW ~74kDa.
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