JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131853

Recombinant Human PRPF31 protein (GST tag N-Terminus)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human PRPF31 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 499 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PRP31, PRPF31, U4/U6 small nuclear ribonucleoprotein Prp31, Pre-mRNA-processing factor 31, Serologically defined breast cancer antigen NY-BR-99, U4/U6 snRNP 61 kDa protein, Protein 61K, hPrp31

1 Images
SDS-PAGE - Recombinant Human PRPF31 protein (GST tag N-Terminus) (AB131853)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PRPF31 protein (GST tag N-Terminus) (AB131853)

12.5% SDS-PAGE analysis of ab131853 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

Q8WWY3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Complementary Products

Primary Antibodies

AB188577

Anti-PRPF31 antibody [EPR14587]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-PRPF31 antibody [EPR14587] (AB188577)

  • 0
  • 0
Primary Antibodies

AB28179

Anti-TFIIF antibody

ChIP - Anti-TFIIF antibody (AB28179)

  • 5
  • 2
Proteins & Peptides

AB132108

Recombinant Human RhoGDI protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human RhoGDI protein (GST tag N-Terminus) (AB132108)

  • 0
  • 0
Primary Antibodies

AB306353

APC Anti-CMTM6 antibody [EPR23015-45]

RabMAb|Recombinant

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKTIAKLWDSKMFAEIMMKIEEYISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVKELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVESRMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIVQSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQRKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARISKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVKGEKSGLMST","proteinLength":"Full Length","predictedMolecularWeight":"81.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":499,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q8WWY3","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRPF31 also known as pre-mRNA processing factor 31 is an important component of the spliceosomal complex. It weights approximately 56 kDa. This protein is expressed in various tissues with prominence in the retina. It plays a critical role in the assembly of the spliceosome by interacting with other splicing factors essential for processing pre-mRNA into mature mRNA.
Biological function summary

PRPF31 is involved in RNA splicing a process fundamental for gene expression. It forms part of the U4/U6-U5 snRNP complex which is responsible for catalyzing the splicing of pre-mRNA. PRPF31 facilitates the rearrangement of RNA and protein components within the spliceosome ensuring accurate splicing and removal of introns.

Pathways

The protein plays a role in the spliceosome pathway and genetic information processing. PRPF31 interacts with other splicing factors like PRPF6 and SNRNP200 within these pathways. Its activity is important for maintaining the fidelity and efficiency of spliceosome assembly and function linking gene regulation and expression to various cellular responses.

Mutations in PRPF31 lead to autosomal dominant retinitis pigmentosa a progressive retinal disorder. This condition connects PRPF31 to other retinal proteins like RP9. Its dysfunction disrupts normal splicing activities causing photoreceptor cell degeneration and vision loss. Understanding PRPF31's role provides insight into therapeutic targets for retinal diseases.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11867543, PubMed : 20118938, PubMed : 28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed : 11867543).

Sequence similarities

Belongs to the PRP31 family.

Post-translational modifications

Phosphorylated by PRP4K during spliceosome assembly.

style
left-column

Product protocols

style
left-column

Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11867543, PubMed : 20118938, PubMed : 28781166). Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome (PubMed : 11867543).
See full target information PRPF31
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com