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AB201398

Recombinant Human PRPF4B protein

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Recombinant Human PRPF4B protein is a Human Fragment protein, in the 551 to 1007 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE.

View Alternative Names

KIAA0536, PRP4, PRP4B, PRP4H, PRPF4K, PRP4K, PRPF4B, Serine/threonine-protein kinase PRP4 homolog, PRP4 kinase, PRP4 pre-mRNA-processing factor 4 homolog

1 Images
SDS-PAGE - Recombinant Human PRPF4B protein (AB201398)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PRPF4B protein (AB201398)

SDS PAGE analysis of ab201398

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q13523

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl, 0.31% Glutathione, 0.29% Sodium chloride, 0.003% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% EDTA, 0.001% PMSF

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Complementary Products

Cell Lines & Lysates

AB267310

Human PRPF4B knockout HEK-293T cell line

Sanger Sequencing - Human PRPF4B knockout HEK-293T cell line (AB267310)

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  • 0
Cell Lines & Lysates

AB259064

Human PRPF4B knockout HEK-293T cell lysate

Sanger Sequencing - Human PRPF4B knockout HEK-293T cell lysate (AB259064)

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  • 0
Primary Antibodies

AB50812

Anti-CA150 antibody [TCEAD79A]

Immunocytochemistry/ Immunofluorescence - Anti-CA150 antibody [TCEAD79A] (AB50812)

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  • 0
Proteins & Peptides

AB316534

Recombinant Human LAMP2 Protein Standard

Sandwich ELISA - Recombinant Human LAMP2 Protein Standard (AB316534)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"KYKYLAEDSNMSVPSEPSSPQSSTRTRSPSPDDILERVAADVKEYERENVDTFEASVKAKHNLMTVEQNNGSSQKKLLAPDMFTESDDMFAAYFDSARLRAAGIGKDFKENPNLRDNWTDAEGYYRVNIGEVLDKRYNVYGYTGQGVFSNVVRARDNARANQEVAVKIIRNNELMQKTGLKELEFLKKLNDADPDDKFHCLRLFRHFYHKQHLCLVFEPLSMNLREVLKKYGKDVGLHIKAVRSYSQQLFLALKLLKRCNILHADIKPDNILVNESKTILKLCDFGSASHVADNDITPYLVSRFYRAPEIIIGKSYDYGIDMWSVGCTLYELYTGKILFPGKTNNHMLKLAMDLKGKMPNKMIRKGVFKDQHFDQNLNFMYIEVDKVTEREKVTVMSTINPTKDLLADLIGCQRLPEDQRKKVHQLKDLLDQILMLDPAKRISINQALQHAFIQEKI","proteinLength":"Fragment","predictedMolecularWeight":"82 kDa","actualMolecularWeight":null,"aminoAcidEnd":1007,"aminoAcidStart":551,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q13523","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Serine/threonine kinase involved in spliceosomal assembly as well as mitosis and signaling regulation (PubMed : 10799319, PubMed : 12077342, PubMed : 17513757, PubMed : 17998396). Connects chromatin mediated regulation of transcription and pre-mRNA splicing (PubMed : 12077342). During spliceosomal assembly, interacts with and phosphorylates PRPF6 and PRPF31, components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP), to facilitate the formation of the spliceosome B complex. Plays a role in regulating transcription and the spindle assembly checkpoint (SAC) (PubMed : 20118938). Associates with U5 snRNP and NCOR1 deacetylase complexes which may allow a coordination of pre-mRNA splicing with chromatin remodeling events involved in transcriptional regulation (PubMed : 12077342). Associates and probably phosphorylates SMARCA4 and NCOR1 (PubMed : 12077342). Phosphorylates SRSF1 (PubMed : 11418604). Associates with kinetochores during mitosis and is necessary for recruitment and maintenance of the checkpoint proteins such as MAD1L1 and MAD12L1 at the kinetochores (PubMed : 17998396). Phosphorylates and regulates the activity of the transcription factors such as ELK1 and KLF13 (PubMed : 10799319, PubMed : 17513757). Phosphorylates nuclear YAP1 and WWTR1/TAZ which induces nuclear exclusion and regulates Hippo signaling pathway, involved in tissue growth control (PubMed : 29695716).

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.

Post-translational modifications

Phosphorylated by CLK1 (PubMed:11418604). Autophosphorylated; phosphorylation inhibits interaction with its targets, such as PRPF6 or SMARCA4 (PubMed:12077342).

Subcellular localisation

Nucleus

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Product protocols

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Target data

Serine/threonine kinase involved in spliceosomal assembly as well as mitosis and signaling regulation (PubMed : 10799319, PubMed : 12077342, PubMed : 17513757, PubMed : 17998396). Connects chromatin mediated regulation of transcription and pre-mRNA splicing (PubMed : 12077342). During spliceosomal assembly, interacts with and phosphorylates PRPF6 and PRPF31, components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP), to facilitate the formation of the spliceosome B complex. Plays a role in regulating transcription and the spindle assembly checkpoint (SAC) (PubMed : 20118938). Associates with U5 snRNP and NCOR1 deacetylase complexes which may allow a coordination of pre-mRNA splicing with chromatin remodeling events involved in transcriptional regulation (PubMed : 12077342). Associates and probably phosphorylates SMARCA4 and NCOR1 (PubMed : 12077342). Phosphorylates SRSF1 (PubMed : 11418604). Associates with kinetochores during mitosis and is necessary for recruitment and maintenance of the checkpoint proteins such as MAD1L1 and MAD12L1 at the kinetochores (PubMed : 17998396). Phosphorylates and regulates the activity of the transcription factors such as ELK1 and KLF13 (PubMed : 10799319, PubMed : 17513757). Phosphorylates nuclear YAP1 and WWTR1/TAZ which induces nuclear exclusion and regulates Hippo signaling pathway, involved in tissue growth control (PubMed : 29695716).
See full target information PRP4K
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