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AB169728

Recombinant Human PRPK protein

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Recombinant Human PRPK protein is a Human Full Length protein, in the 1 to 253 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for SDS-PAGE, WB.

View Alternative Names

C20orf64, PRPK, TP53RK, EKC/KEOPS complex subunit TP53RK, Atypical serine/threonine protein kinase TP53RK, Nori-2, TP53-regulating kinase, p53-related protein kinase

1 Images
SDS-PAGE - Recombinant Human PRPK protein (AB169728)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PRPK protein (AB169728)

The purity of ab169728 was determined by SDS-PAGE.

Key facts

Purity

>80% SDS-PAGE

Affinity purified.

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

Q96S44

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.04% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Proteins & Peptides

AB153791

Recombinant Human VMO1 protein

SDS-PAGE - Recombinant Human VMO1 protein (AB153791)

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Primary Antibodies

AB279377

Anti-PRPK antibody [OTI5B2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRPK antibody [OTI5B2] (AB279377)

  • 0
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Proteins & Peptides

AB199575

Recombinant Human UBA5 protein (His tag)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MAAARATTPADGEEPAPEAEALAAARERSSRFLSGLELVKQGAEARVFRGRFQGRAAVIKHRFPKGYRHPALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLSNLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLSTHPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG","proteinLength":"Full Length","predictedMolecularWeight":"28 kDa","actualMolecularWeight":null,"aminoAcidEnd":253,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q96S44","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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General info

Function

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed : 22912744, PubMed : 27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed : 22912744, PubMed : 27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed : 11546806).

Sequence similarities

Belongs to the protein kinase superfamily. BUD32 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed : 22912744, PubMed : 27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed : 22912744, PubMed : 27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed : 11546806).
See full target information TP53RK
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Product promise

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