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AB113577

Recombinant Human PSMB10/MECL1 protein

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Recombinant Human PSMB10/MECL1 protein is a Human Full Length protein, in the 40 to 237 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

LMP10, MECL1, PSMB10, Proteasome subunit beta type-10, Low molecular mass protein 10, Macropain subunit MECl-1, Multicatalytic endopeptidase complex subunit MECl-1, Proteasome MECl-1, Proteasome subunit beta-2i

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SDS-PAGE - Recombinant Human PSMB10/MECL1 protein (AB113577)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PSMB10/MECL1 protein (AB113577)

15% SDS-PAGE showing ab113577 at approximately 26.9kDa (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P40306

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.02% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PSMB10
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE","proteinLength":"Full Length","predictedMolecularWeight":"26.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":237,"aminoAcidStart":40,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P40306","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMB10 also known as MECL1 is a proteasome component involved in protein degradation. This protein has a molecular mass of approximately 29 kDa. PSMB10 is expressed in various tissues with notable expression in the immune system. As a beta subunit of the 20S proteasome MECL1 has a role in cleaving peptides at specific sites contributing to the breakdown of ubiquitinated proteins.
Biological function summary

PSMB10/MECL1 plays a significant role in immune responses. It forms part of the immunoproteasome a variant of the proteasome complex which enhances the generation of antigenic peptides presented on MHC class I molecules. This function is essential for effective immune surveillance and initializing adaptive immunity. In particular PSMB10's influence on antigen processing positions it as an important player in modulating immune system activity.

Pathways

PSMB10/MECL1 participates in pathways associated with protein catabolism and immune signaling. It is involved in the ubiquitin-proteasome pathway which is critical for maintaining cellular protein balance. The function of PSMB10 within the immunoproteasome links it to immune signaling pathways. It has associations with other 20S proteasome subunits such as PSMB8 and PSMB9 which also contribute to the formation of the immunoproteasome complex.

PSMB10/MECL1 has connections to autoimmune diseases and cancer. In autoimmune diseases the altered function of PSMB10 within the immunoproteasome can lead to improper antigen presentation potentially triggering excessive immune responses. In oncology aberrant expression of immunoproteasome subunits including PSMB10 has implications in tumor immune evasion. The interplay with proteins like PSMB9 in these contexts suggests an intricate network affecting disease progression and immune regulation.
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Specifications

Form

Liquid

Additional notes

ab113577 is purified using conventional chromatography techniques.

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General info

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Sequence similarities

Belongs to the peptidase T1B family.

Post-translational modifications

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.

Subcellular localisation

Nucleus

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Product protocols

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Target data

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.
See full target information PSMB10
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Complementary Products

Proteins & Peptides

AB115721

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Primary Antibodies

AB183506

Anti-PSMB10/MECL1 antibody [EPR14902]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PSMB10/MECL1 antibody [EPR14902] (AB183506)

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Assay Kits

AB112154

Proteasome 20S Activity Assay Kit (Fluorometric)

Functional Studies - Proteasome 20S Activity Assay Kit (Fluorometric) (AB112154)

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Primary Antibodies

AB272692

Anti-MUC2 antibody [EPR23479-47]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|Trial Size

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692)

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