Recombinant Human PSMB4 protein is a Human Full Length protein, in the 46 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M T Q N P M V T G T S V L G V K F E G G V V I A A D M L G S Y G S L A R F R N I S R I M R V N N S T M L G A S G D Y A D F Q Y L K Q V L G Q M V I D E E L L G D G H S Y S P R A I H S W L T R A M Y S R R S K M N P L W N T M V I G G Y A D G E S F L G Y V D M L G V A Y E A P S L A T G Y G A Y L A Q P L L R E V L E K Q P V L S Q T E A R D L V E R C M R V L Y Y R D A R S Y N R F Q I A T V T E K G V E I E G P L S T E T N W D I A H M I S G F E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
Alternative names
PROS26, PSMB4, Proteasome subunit beta type-4, 26 kDa prosomal protein, Macropain beta chain, Multicatalytic endopeptidase complex beta chain, Proteasome beta chain, Proteasome chain 3, Proteasome subunit beta-7, HsBPROS26, PROS-26, HsN3, beta-7
Recommended products
Recombinant Human PSMB4 protein is a Human Full Length protein, in the 46 to 264 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P28070-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M T Q N P M V T G T S V L G V K F E G G V V I A A D M L G S Y G S L A R F R N I S R I M R V N N S T M L G A S G D Y A D F Q Y L K Q V L G Q M V I D E E L L G D G H S Y S P R A I H S W L T R A M Y S R R S K M N P L W N T M V I G G Y A D G E S F L G Y V D M L G V A Y E A P S L A T G Y G A Y L A Q P L L R E V L E K Q P V L S Q T E A R D L V E R C M R V L Y Y R D A R S Y N R F Q I A T V T E K G V E I E G P L S T E T N W D I A H M I S G F E
- Accession
- P28070
- Protein length
- Full Length
- Predicted molecular weight
- 26.6 kDa
- Amino acids
- 46 to 264
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab115721 was purified using conventional chromatography techniques.
General info
- Function
Non-catalytic component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
- Sequence similarities
Belongs to the peptidase T1B family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as Proteasome subunit beta type 4
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSMB4 also known as proteasome subunit beta type-4 is a critical component of the 20S proteasome complex involved in protein degradation. Its molecular mass is approximately 29 kDa. PSMB4 expression occurs widely including in the cytoplasm and nucleus of eukaryotic cells. It plays an important role in regulating protein turnover by participating in the breakdown of ubiquitinated proteins essential for maintaining cellular protein homeostasis.
- Biological function summary
The 20S proteasome where PSMB4 belongs acts as the proteolytic core of the 26S proteasome complex. This complex degrades unwanted or damaged proteins thereby regulating various cellular processes such as the cell cycle apoptosis and DNA repair. PSMB4's activity ensures the removal of potentially toxic proteins which is important for cell function and survival.
- Pathways
PSMB4 is involved in key pathways such as the ubiquitin-proteasome pathway and antigen processing. These pathways regulate protein metabolism and assist in immune surveillance by presenting endogenous antigens through MHC class I molecules. PSMB4 interacts closely with other proteasome subunits like PSMB5 and PSMB6 which coordinate to cleave peptide bonds in substrate proteins effectively.
- Associated diseases and disorders
Mutations or dysregulation of PSMB4 link to multiple myeloma and neurodegenerative diseases. In multiple myeloma changes in PSMB4 may affect proteasome inhibitor effectiveness while in neurodegenerative disorders impaired protein degradation can lead to protein aggregation. PSMB4 has potential interactions with proteins such as ubiquitin and PSMC3 which further influence disease progression.
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1 product image
SDS-PAGE - Recombinant Human PSMB4 protein (ab115721)
15% SDS-PAGE showing ab115721 at approximately 26.6kDa (3μg).
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