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AB101825

Recombinant Human PSMB5/MB1 protein (His tag N-Terminus)

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Recombinant Human PSMB5/MB1 protein (His tag N-Terminus) is a Human Full Length protein, in the 60 to 263 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

LMPX, MB1, X, PSMB5, Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, Proteasome subunit beta-5, beta-5

1 Images
SDS-PAGE - Recombinant Human PSMB5/MB1 protein (His tag N-Terminus) (AB101825)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PSMB5/MB1 protein (His tag N-Terminus) (AB101825)

15% SDS-PAGE analysis of ab101825 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P28074

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.876% Sodium chloride, 0.316% Tris HCl, 0.015% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as PSMB5.
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Sequence info

[{"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMTTTLAFKFRHGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELRNKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVGSGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSSDNVADLHEKYSGSTP","proteinLength":"Full Length","predictedMolecularWeight":"26.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":263,"aminoAcidStart":60,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P28074","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMB5 also known as MB1 or β5 is a subunit of the 20S proteasome complex with a molecular mass of approximately 28 kDa. This protein functions as a chymotrypsin-like peptidase that is important for protein degradation. It is expressed in various tissues throughout the body particularly in those with high protein turnover.
Biological function summary

PSMB5 plays a significant role in regulating protein homeostasis within the cell. It forms part of the core 20S proteasome complex where its enzymatic activity facilitates the breakdown of ubiquitinated proteins into smaller peptides. This protein degradation is essential for many cellular processes including the removal of damaged or misfolded proteins therefore maintaining cellular function.

Pathways

PSMB5 is integral to the ubiquitin-proteasome pathway which is important for protein recycling and quality control within cells. This pathway involves the tagging of proteins with ubiquitin and their subsequent degradation by the proteasome. PSMB5 interacts with other proteasome subunits like β1 and β2 contributing to a functional protein breakdown machinery. Dysregulation of this pathway can lead to the accumulation of unwanted proteins and affect cellular health.

Alterations in PSMB5 function have been implicated in the development of cancer and neurodegenerative diseases. Cancer cells often exhibit altered proteasome activity where PSMB5 may contribute to the survival and proliferation of these cells. In neurodegenerative diseases like Alzheimer's impairments in protein degradation pathways including PSMB5 can lead to protein aggregation and toxicity. Researchers are exploring how PSMB5 interacts with other proteins within these contexts to better understand its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab101825 was purified using conventional chromatography techniques.

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General info

Function

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.

Sequence similarities

Belongs to the peptidase T1B family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB5 displays a chymotrypsin-like activity.
See full target information PSMB5
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