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AB174394

Recombinant Human PSMD11 protein (denatured) (His tag N-Terminus)

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Recombinant Human PSMD11 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 422 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p44.5, PSMD11

1 Images
SDS-PAGE - Recombinant Human PSMD11 protein (denatured) (His tag N-Terminus) (AB174394)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PSMD11 protein (denatured) (His tag N-Terminus) (AB174394)

SDS-PAGE analysis of PSMD11 using 3 μg of ab174394 on a 15% gel.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O00231

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris-HCl buffer

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at 3 μg/lane</p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAAAAVVEFQRAQSLLSTDREASIDILHSIVKRDIQENDEEAVQVKEQSILELGSLLAKTGQAAELGGLLKYVRPFLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT","proteinLength":"Full Length","predictedMolecularWeight":"49.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":422,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O00231","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PSMD11 also known as RPN-6 is a regulatory subunit of the proteasome 26S. It is approximately 50 kDa in mass and plays an essential role in the proteasome's function responsible for protein degradation within cells. PSMD11 is broadly expressed in various tissues including high expression in the brain heart and skeletal muscle. Its mechanical role involves binding to other subunits to stabilize the proteasome structure and facilitate substrate entry into the proteolytic core.
Biological function summary

PSMD11 is essential for the proper assembly and activity of the 26S proteasome complex which is important for ubiquitin-mediated proteolysis. This process controls protein turnover and regulates the concentration of various proteins to maintain cellular homeostasis. PSMD11 enhances the degradation of misfolded or damaged proteins therefore preventing the accumulation of toxic protein aggregates within cells. Its expression levels can modulate the proteasome activity in response to cellular conditions.

Pathways

PSMD11 plays a significant role in the ubiquitin-proteasome pathway which is critical for protein homeostasis and degradation of regulatory proteins. This pathway involves several important proteins including ubiquitin which tags substrates for degradation. PSMD11 also interfaces with signaling pathways that regulate cell cycle progression and apoptosis such as interactions with p53 and c-Myc proteins. These pathways ensure the balance between cell survival and programmed cell death.

PSMD11 links directly to conditions involving proteasome dysfunction such as neurodegenerative diseases including Parkinson's disease and Alzheimer's disease. Dysregulation of PSMD11 can impact proteasome activity leading to the buildup of unwanted proteins that contribute to these conditions. Additionally PSMD11's interaction with proteins like alpha-synuclein and tau in these diseases highlights its relevance in understanding their pathogenesis.
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Specifications

Form

Liquid

Additional notes

ab174394 is purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.

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General info

Function

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs) : its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.

Sequence similarities

Belongs to the proteasome subunit S9 family.

Post-translational modifications

Phosphorylated by AMPK.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs) : its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
See full target information PSMD11
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