Recombinant Human PSME3 protein is a Human Full Length protein, in the 1 to 254 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A S L L K V D Q E V K L K V D S F R E R I T S E A E D L V A N F F P K K L L E L D S F L K E P I L N I H D L T Q I H S D M N L P V P D P I L L T N S H D G L D G P T Y K K R R L D E C E E A F Q G T K V F V M P N G M L K S N Q Q L V D I I E K V K P E I R L L I E K C N T V K M W V Q L L I P R I E D G N N F G V S I Q E E T V A E L R T V E S E A A S Y L D Q I S R Y Y I T R A K L V S K I A K Y P H V E D Y R R T V T E I D E K E Y I S L R L I I S E L R N Q Y V T L H D M I L K N I E K I K R P R S S N A E T L Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).
Alternative names
Proteasome activator complex subunit 3, 11S regulator complex subunit gamma, Activator of multicatalytic protease subunit 3, Ki nuclear autoantigen, Proteasome activator 28 subunit gamma, REG-gamma, PA28g, PA28gamma, PSME3
Recommended products
Recombinant Human PSME3 protein is a Human Full Length protein, in the 1 to 254 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P61289-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A S L L K V D Q E V K L K V D S F R E R I T S E A E D L V A N F F P K K L L E L D S F L K E P I L N I H D L T Q I H S D M N L P V P D P I L L T N S H D G L D G P T Y K K R R L D E C E E A F Q G T K V F V M P N G M L K S N Q Q L V D I I E K V K P E I R L L I E K C N T V K M W V Q L L I P R I E D G N N F G V S I Q E E T V A E L R T V E S E A A S Y L D Q I S R Y Y I T R A K L V S K I A K Y P H V E D Y R R T V T E I D E K E Y I S L R L I I S E L R N Q Y V T L H D M I L K N I E K I K R P R S S N A E T L Y
- Accession
- P61289
- Protein length
- Full Length
- Predicted molecular weight
- 31.7 kDa
- Amino acids
- 1 to 254
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab115714 was purified using conventional chromatography techniques.
General info
- Function
Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition (PubMed:25361978).
- Sequence similarities
Belongs to the PA28 family.
- Post-translational modifications
Phosphorylated by MAP3K3 (By similarity). Phosphorylation at Ser-247 promotes its association with CCAR2.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PSME3 protein also known as PA28γ or REGγ is mechanically an essential proteasome activator that facilitates the degradation of ubiquitin-independent substrates. It has a molecular weight of approximately 29 kDa. PSME3 is mainly found in the nucleus and shows strong expression in various tissues including liver kidney and heart. The protein operates by binding to the 20S proteasome core and enhances the specificity of proteolytic activity.
- Biological function summary
PSME3 regulates cell cycle progression and apoptosis. It is part of a larger multiprotein complex with the 20S proteasome core forming an important component in protein homeostasis. PSME3’s interaction with the proteasome core significantly reduces the accumulation of certain regulatory proteins by promoting their degradation. This function affects several cellular processes such as cell proliferation and DNA repair aiding the maintenance of cellular function and integrity.
- Pathways
PSME3 plays a critical role in mediating the ubiquitin-proteasome pathway and the MDM2-p53 pathway. In the ubiquitin-proteasome pathway it assists in the controlled turnover of intracellular proteins which is pivotal for maintaining cellular protein quality. In the MDM2-p53 pathway it affects the stability of the p53 protein by enhancing the degradation of both MDM2 and p53 therefore modulating apoptosis and growth arrest in cells under stress or DNA damage conditions.
- Associated diseases and disorders
Disruptions in PSME3 function correlate with cancer and neurodegenerative disorders. Overexpression of PSME3 is often linked to oncogenesis due to its role in controlling the degradation of tumor suppressor proteins like p53. This protein is also implicated in Parkinson’s disease where its interaction with pathogenic proteins suggests it might contribute to the accumulation of misfolded proteins. PSME3 along with its pathways and related proteins offers potential as a target for therapeutic intervention in these diseases.
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SDS-PAGE - Recombinant Human PSME3 protein (ab115714)
SDS-PAGE analysis of ab115714 (3µg).
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