Recombinant human PSPN protein (Active) is a Human Fragment protein, in the 61 to 156 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
Amino acid sequence
M A L S G P C Q L W S L T L S V A E L G L G Y A S E E K V I F R Y C A G S C P R G A R T Q H G L A L A R L Q G Q G R A H G G P C C R P T R Y T D V A F L D D R H R W Q R L P Q L S A A A C G C G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.
Alternative names
Persephin, PSP, PSPN
Recommended products
Recombinant human PSPN protein (Active) is a Human Fragment protein, in the 61 to 156 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
Determined by TT cell proliferation assay ED50 ≤20 ng/mL (≥ 5.0 x 104 units/mg) (lot specific).
- Accession
- O60542-1
- Animal free
No
- Carrier free
Yes
- Species
Human
- Reconstitution
Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.16% Sodium phosphate
Sequence info
Amino acid sequence
M A L S G P C Q L W S L T L S V A E L G L G Y A S E E K V I F R Y C A G S C P R G A R T Q H G L A L A R L Q G Q G R A H G G P C C R P T R Y T D V A F L D D R H R W Q R L P Q L S A A A C G C G G
- Accession
- Protein length
Fragment
- Predicted molecular weight
10.4 kDa
- Amino acids
61 to 156
- Nature
Recombinant
Specifications
- Form
Lyophilized
General info
- Function
Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.
- Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSPN also known as Persephin is a member of the glial cell line-derived neurotrophic factor (GDNF) family with a molecular mass of approximately 11 kDa. This protein is primarily secreted and expressed in several tissues including the central nervous system showing notable localization in the spinal cord and brain regions such as the thalamus. PSPN specifically interacts with cell surface receptors and mediates survival and differentiation of neuronal populations similar in function to other GDNF family members.
- Biological function summary
PSPN plays an important role in neuroprotective processes. It does not function as part of a larger complex but instead binds to a receptor complex involving GFR alpha-4 and RET receptor tyrosine kinase. Through this interaction PSPN promotes neuronal survival and influences cell fate decisions particularly within dopaminergic and motor neurons providing critical support for their maintenance and function.
- Pathways
PSPN participates in the signaling cascades that are essential for neural development and repair. It is primarily embedded in the RET signaling pathway which is fundamental to the activation of intracellular processes like cell survival and differentiation. PSPN shares functional characteristics with other members of the GDNF family including GDNF and Neurturin as these ligands also interact with RET signaling components to activate downstream events necessary for neuron health.
- Associated diseases and disorders
Malfunction or dysregulation of PSPN expression associates with neurodegenerative diseases such as Parkinson's disease and amyotrophic lateral sclerosis (ALS). These conditions involve the loss of specific neuronal populations where PSPN's role in promoting cell survival becomes significantly impactful. PSPN operates in tandem with other neurotrophic factors like GDNF contributing to potential therapeutic strategies aimed at preserving neuronal functionality and mitigating the effects of these debilitating diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant human PSPN protein (Active) (ab218092)
SDS-PAGE analysis of ab218092 (1 μg) stained with Coomassie blue:
Lane 1: Non-reduced
Lane 2: Reduced
4-20% Tris-Glycine gel
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com