Recombinant human PSPN protein (Active) (Animal Free) is a Human Fragment protein, in the 61 to 156 aa range, expressed in Escherichia coli, with >97% purity, < 0.05 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 0.05 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M A L S G P C Q L W S L T L S V A E L G L G Y A S E E K V I F R Y C A G S C P R G A R T Q H G L A L A R L Q G Q G R A H G G P C C R P T R Y T D V A F L D D R H R W Q R L P Q L S A A A C G C G G
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Growth factor that exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons (PubMed:11116144, PubMed:9491986). Acts by binding to its coreceptor, GFRA4, leading to autophosphorylation and activation of the RET receptor (PubMed:11116144).
Alternative names
Persephin, PSP, PSPN
Recombinant human PSPN protein (Active) (Animal Free) is a Human Fragment protein, in the 61 to 156 aa range, expressed in Escherichia coli, with >97% purity, < 0.05 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Endotoxin level
- < 0.05 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The activity is determined by a cell proliferation assay with TT Human medullary thyroid cancer cells and is typically 4-16 ng/mL. This corresponds to an expected specific activity of 2.5 x 105 units/mg.
- Accession
- O60542-1
- Animal free
- Yes
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in water
- Concentration
- Storage buffer
Constituents: 0.16% Sodium phosphate
Sequence info
Amino acid sequence
- M A L S G P C Q L W S L T L S V A E L G L G Y A S E E K V I F R Y C A G S C P R G A R T Q H G L A L A R L Q G Q G R A H G G P C C R P T R Y T D V A F L D D R H R W Q R L P Q L S A A A C G C G G
- Accession
- O60542
- Protein length
- Fragment
- Predicted molecular weight
- 10.4 kDa
- Amino acids
- 61 to 156
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
The purity of ab218098 is typically 97% as determined by reducing and non-reducing SDS-PAGE.
General info
- Function
Growth factor that exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons (PubMed:11116144, PubMed:9491986). Acts by binding to its coreceptor, GFRA4, leading to autophosphorylation and activation of the RET receptor (PubMed:11116144).
- Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PSPN also known as Persephin is a member of the glial cell line-derived neurotrophic factor (GDNF) family with a molecular mass of approximately 11 kDa. This protein is primarily secreted and expressed in several tissues including the central nervous system showing notable localization in the spinal cord and brain regions such as the thalamus. PSPN specifically interacts with cell surface receptors and mediates survival and differentiation of neuronal populations similar in function to other GDNF family members.
- Biological function summary
PSPN plays an important role in neuroprotective processes. It does not function as part of a larger complex but instead binds to a receptor complex involving GFR alpha-4 and RET receptor tyrosine kinase. Through this interaction PSPN promotes neuronal survival and influences cell fate decisions particularly within dopaminergic and motor neurons providing critical support for their maintenance and function.
- Pathways
PSPN participates in the signaling cascades that are essential for neural development and repair. It is primarily embedded in the RET signaling pathway which is fundamental to the activation of intracellular processes like cell survival and differentiation. PSPN shares functional characteristics with other members of the GDNF family including GDNF and Neurturin as these ligands also interact with RET signaling components to activate downstream events necessary for neuron health.
- Associated diseases and disorders
Malfunction or dysregulation of PSPN expression associates with neurodegenerative diseases such as Parkinson's disease and amyotrophic lateral sclerosis (ALS). These conditions involve the loss of specific neuronal populations where PSPN's role in promoting cell survival becomes significantly impactful. PSPN operates in tandem with other neurotrophic factors like GDNF contributing to potential therapeutic strategies aimed at preserving neuronal functionality and mitigating the effects of these debilitating diseases.
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1 product image
SDS-PAGE - Recombinant human PSPN protein (Active) (Animal Free) (ab218098)
SDS-PAGE analysis of ab218098 (1μg) stained with Coomassie blue:
Lane 1: non-reduced
Lane 2: reduced
4-20% Tris-Glycine gel
Human PSPN is predicted to be a dimer (20.8 kDa)
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