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Recombinant Human PSTPIP1 protein is a Human Full Length protein, in the 1 to 416 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names=Proline-serine-threonine phosphatase-interacting protein 1, PEST phosphatase-interacting protein 1, CD2-binding protein 1, H-PIP, PSTPIP1, CD2BP1
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M G S M M P Q L Q F K D A F W C R D F T A H T G Y E V L L Q R L L D G R K M C K D M E E L L R Q R A Q A E E R Y G K E L V Q I A R K A G G Q T E I N S L R A S F D S L K Q Q M E N V G S S H I Q L A L T L R E E L R S L E E F R E R Q K E Q R K K Y E A V M D R V Q K S K L S L Y K K A M E S K K T Y E Q K C R D A D D A E Q A F E R I S A N G H Q K Q V E K S Q N K A R Q C K D S A T E A E R V Y R Q S I A Q L E K V R A E W E Q E H R T T C E A F Q L Q E F D R L T I L R N A L W V H S N Q L S M Q C V K D D E L Y E E V R L T L E G C S I D A D I D S F I Q A K S T G T E P P A P V P Y Q N Y Y D R E V T P L T S S P G I Q P S C G M I K R F S G L L H G S P K T T S L A A S A A S T E T L T P T P E R N E G V Y T A I A V Q E I Q G N P A S P A Q E Y R A L Y D Y T A Q N P D E L D L S A G D I L E V I L E G E D G W W T V E R N G Q R G F V P G S Y L E K L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human PSTPIP1 protein is a Human Full Length protein, in the 1 to 416 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names=Proline-serine-threonine phosphatase-interacting protein 1, PEST phosphatase-interacting protein 1, CD2-binding protein 1, H-PIP, PSTPIP1, CD2BP1
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
50 kDa
- Amino acids
1 to 416
- Sequence
M G S S H H H H H H S S G L V P R G S H M G S M M P Q L Q F K D A F W C R D F T A H T G Y E V L L Q R L L D G R K M C K D M E E L L R Q R A Q A E E R Y G K E L V Q I A R K A G G Q T E I N S L R A S F D S L K Q Q M E N V G S S H I Q L A L T L R E E L R S L E E F R E R Q K E Q R K K Y E A V M D R V Q K S K L S L Y K K A M E S K K T Y E Q K C R D A D D A E Q A F E R I S A N G H Q K Q V E K S Q N K A R Q C K D S A T E A E R V Y R Q S I A Q L E K V R A E W E Q E H R T T C E A F Q L Q E F D R L T I L R N A L W V H S N Q L S M Q C V K D D E L Y E E V R L T L E G C S I D A D I D S F I Q A K S T G T E P P A P V P Y Q N Y Y D R E V T P L T S S P G I Q P S C G M I K R F S G L L H G S P K T T S L A A S A A S T E T L T P T P E R N E G V Y T A I A V Q E I Q G N P A S P A Q E Y R A L Y D Y T A Q N P D E L D L S A G D I L E V I L E G E D G W W T V E R N G Q R G F V P G S Y L E K L
- Accession
- O43586-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
ab167828 was purified using conventional chromatography techniques.
General info
- Function
Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (By similarity). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.
- Post-translational modifications
Dephosphorylated on Tyr-345 by PTPN18, this event negatively regulates the association of PSTPIP1 with SH2 domain-containing proteins as tyrosine kinase. Phosphorylation of Tyr-345 is probably required for subsequent phosphorylation at other tyrosine residues. Phosphorylation is induced by activation of the EGFR and PDGFR in a ABL1 dependent manner. The phosphorylation regulates the interaction with WAS and with MEFV (By similarity).
- Subcellular localisation
Cytoskeleton
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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