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AB107954

Recombinant Human PTGES2/Gbf1 protein

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Recombinant Human PTGES2/Gbf1 protein is a Human Full Length protein, in the 1 to 206 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

C9orf15, PGES2, PTGES2, Prostaglandin E synthase 2, Membrane-associated prostaglandin E synthase-2, Microsomal prostaglandin E synthase 2, Prostaglandin-H(2) E-isomerase, mPGE synthase-2, mPGES-2

1 Images
SDS-PAGE - Recombinant Human PTGES2/Gbf1 protein (AB107954)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PTGES2/Gbf1 protein (AB107954)

15% SDS-PAGE analysis of ab107954 (3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9H7Z7

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as PTGES2
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEASPAH","proteinLength":"Full Length","predictedMolecularWeight":"23.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":206,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9H7Z7","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PTGES2 also known as Gbf1 is a member of the prostaglandin E synthase family and functions as an enzyme involved in the conversion of PGH2 to PGE2 an essential step in prostaglandin biosynthesis. The protein has an approximate mass of 40 kDa. PTGES2 is expressed across various tissues with higher levels observed in the brain kidney and liver. The enzyme operates optimally in the presence of reduced glutathione which is necessary for its activity.
Biological function summary

PTGES2 plays an important role in the synthesis of prostaglandins which are lipid compounds that perform critical functions in inflammation and other cellular processes. As part of the prostaglandin synthesis pathway PTGES2 interacts cooperatively with other enzymes such as cyclooxygenase-2 (COX-2) to produce PGE2. PGE2 is significant for regulating immune responses and maintaining homeostasis. Disruptions in prostaglandin production can lead to various inflammatory disorders.

Pathways

PTGES2 is integral to the arachidonic acid metabolic pathway where it acts in unison with COX-2 and other enzymes to convert arachidonic acid into bioactive prostaglandins. Specifically its role in the PGE2 synthesis pathway is well-documented which ties it closely to proteins involved in eicosanoid synthesis. PGE2 the product of this pathway modulates both pain and inflammation showing how PTGES2 impacts physiological processes beyond its enzymatic function.

PTGES2 has been linked to inflammatory conditions such as rheumatoid arthritis and cancer. Its activity correlates with increased levels of PGE2 a molecule known for promoting inflammation and tumorigenesis. Associations with COX-2 have been studied extensively in the context of cancer especially in relation to its impact on cellular proliferation and immune evasion. As our understanding of PTGES2 continues to evolve its potential as a therapeutic target for inflammatory and neoplastic diseases is being actively investigated.
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Specifications

Form

Liquid

Additional notes

ab107954 was purified by using conventional chromatography techniques.

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General info

Function

Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (in vitro) (PubMed : 12804604, PubMed : 17585783, PubMed : 18198127). The biological function and the GSH-dependent property of PTGES2 is still under debate (PubMed : 17585783, PubMed : 18198127). In vivo, PTGES2 could form a complex with GSH and heme and would not participate in PGE2 synthesis but would catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) (By similarity) (PubMed : 17585783).

Sequence similarities

Belongs to the GST superfamily.

Post-translational modifications

Synthesized as a Golgi membrane-associated protein, and the proteolytic removal of the N-terminal hydrophobic domain leads to the formation of a mature cytosolic enzyme.

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Product protocols

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Target data

Isomerase that catalyzes the conversion of PGH2 into the more stable prostaglandin E2 (PGE2) (in vitro) (PubMed : 12804604, PubMed : 17585783, PubMed : 18198127). The biological function and the GSH-dependent property of PTGES2 is still under debate (PubMed : 17585783, PubMed : 18198127). In vivo, PTGES2 could form a complex with GSH and heme and would not participate in PGE2 synthesis but would catalyze the degradation of prostaglandin E2 H2 (PGH2) to 12(S)-hydroxy-5(Z),8(E),10(E)-heptadecatrienoic acid (HHT) and malondialdehyde (MDA) (By similarity) (PubMed : 17585783).
See full target information PTGES2
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