Recombinant human PTP1B protein (Active) is a Human Full Length protein, in the 1 to 435 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M E M E K E F E Q I D K S G S W A A I Y Q D I R H E A S D F P C R V A K L P K N K N R N R Y R D V S P F D H S R I K L H Q E D N D Y I N A S L I K M E E A Q R S Y I L T Q G P L P N T C G H F W E M V W E Q K S R G V V M L N R V M E K G S L K C A Q Y W P Q K E E K E M I F E D T N L K L T L I S E D I K S Y Y T V R Q L E L E N L T T Q E T R E I L H F H Y T T W P D F G V P E S P A S F L N F L F K V R E S G S L S P E H G P V V V H C S A G I G R S G T F C L A D T C L L L M D K R K D P S S V D I K K V L L E M R K F R M G L I Q T A D Q L R F S Y L A V I E G A K F I M G D S S V Q D Q W K E L S H E D L E P P P E H I P P P P R P P K R I L E P H N G K C R E F F P N H Q W V K E E T Q E D K D C P I K E E K G S P L N A A P Y G I E S M S Q D T E V R S R V V G G S L R G A Q A A S P A K G E P S L P E K D E D H A L S Y W K P F L V N M C V A T V L T A G A Y L C Y R F L F N S N T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Alternative names
PTP1B, PTPN1, Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B
Recommended products
Recombinant human PTP1B protein (Active) is a Human Full Length protein, in the 1 to 435 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab268897 was ~2,640 nmol/min/mg in a phosphatase assay using pNPP as substrate.
- Accession
- P18031-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.42% MOPS, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M E M E K E F E Q I D K S G S W A A I Y Q D I R H E A S D F P C R V A K L P K N K N R N R Y R D V S P F D H S R I K L H Q E D N D Y I N A S L I K M E E A Q R S Y I L T Q G P L P N T C G H F W E M V W E Q K S R G V V M L N R V M E K G S L K C A Q Y W P Q K E E K E M I F E D T N L K L T L I S E D I K S Y Y T V R Q L E L E N L T T Q E T R E I L H F H Y T T W P D F G V P E S P A S F L N F L F K V R E S G S L S P E H G P V V V H C S A G I G R S G T F C L A D T C L L L M D K R K D P S S V D I K K V L L E M R K F R M G L I Q T A D Q L R F S Y L A V I E G A K F I M G D S S V Q D Q W K E L S H E D L E P P P E H I P P P P R P P K R I L E P H N G K C R E F F P N H Q W V K E E T Q E D K D C P I K E E K G S P L N A A P Y G I E S M S Q D T E V R S R V V G G S L R G A Q A A S P A K G E P S L P E K D E D H A L S Y W K P F L V N M C V A T V L T A G A Y L C Y R F L F N S N T
- Accession
- P18031
- Protein length
- Full Length
- Amino acids
- 1 to 435
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
- Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
- Post-translational modifications
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein tyrosine phosphatase 1B (PTP1B) is an enzyme involved in the dephosphorylation of tyrosine residues in proteins. Known also as PTPN1 this protein has a molecular weight of approximately 50 kDa. PTP1B is widely expressed in tissues but is highly present in the liver muscle and adipose tissue. It plays a critical role in regulating signal transduction pathways by removing phosphate groups from phosphotyrosine residues on target proteins. This action generally serves as a 'turn-off' switch for various signaling pathways.
- Biological function summary
PTP1B plays important roles in glucose and lipid metabolism. It is not generally considered a part of a complex functioning instead as a singular enzyme. Its main action involves the negative regulation of the insulin signaling pathway by dephosphorylating the insulin receptor as well as its associated substrates. This regulation helps maintain normal levels of insulin sensitivity influencing cellular glucose uptake and metabolism.
- Pathways
PTP1B has important functions in the insulin and leptin signaling pathways. It has a regulatory influence in these pathways affecting key metabolic processes. In the insulin signaling pathway PTP1B closely interacts with the insulin receptor as well as with insulin receptor substrates (IRS). Its dephosphorylating activity can lead to decreased insulin sensitivity. In the leptin pathway PTP1B modulates leptin signaling by dephosphorylating the Janus kinase 2 (JAK2) another protein involved in the control of energy balance and metabolism.
- Associated diseases and disorders
Excessive activity of PTP1B links with disorders such as type 2 diabetes and obesity. Overactivity of PTP1B can lead to insulin resistance contributing to the pathogenesis of type 2 diabetes. In obesity PTP1B may interfere with the normal activity of leptin a hormone critical for regulating energy balance. Its interaction with the insulin receptor and JAK2 makes PTP1B a target for therapeutic interventions aimed at improving insulin sensitivity and energy homeostasis.
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2 product images
Functional Studies - Recombinant human PTP1B protein (Active) (ab268897)
The specific activity of ab268897 was ~2,640 nmol/min/mg in a phosphatase assay using pNPP as substrate.
SDS-PAGE - Recombinant human PTP1B protein (Active) (ab268897)
SDS-PAGE analysis of ab268897.
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