Recombinant Human PTPN12 protein is a Human Full Length protein, in the 1 to 780 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M E Q V E I L R K F I Q R V Q A M K S P D H N G E D N F A R D F M R L R R L S T K Y R T E K I Y P T A T G E K E E N V K K N R Y K D I L P F D H S R V K L T L K T P S Q D S D Y I N A N F I K G V Y G P K A Y V A T Q G P L A N T V I D F W R M I W E Y N V V I I V M A C R E F E M G R K K C E R Y W P L Y G E D P I T F A P F K I S C E D E Q A R T D Y F I R T L L L E F Q N E S R R L Y Q F H Y V N W P D H D V P S S F D S I L D M I S L M R K Y Q E H E D V P I C I H C S A G C G R T G A I C A I D Y T W N L L K A G K I P E E F N V F N L I Q E M R T Q R H S A V Q T K E Q Y E L V H R A I A Q L F E K Q L Q L Y E I H G A Q K I A D G V N E I N T E N M I S S I E P E K Q D S P P P K P P R T R S C L V E G D A K E E I L Q P P E P H P V P P I L T P S P P S A F P T V T T V W Q D N D R Y H P K P V L H M V S S E Q H S A D L N R N Y S K S T E L P G K N E S T I E Q I D K K L E R N L S F E I K K V P L Q E G P K S F D G N T L L N R G H A I K I K S A S P C I A D K I S K P Q E L S S D L N V G D T S Q N S C V D C S V T Q S N K V S V T P P E E S Q N S D T P P R P D R L P L D E K G H V T W S F H G P E N A I P I P D L S E G N S S D I N Y Q T R K T V S L T P S P T T Q V E T P D L V D H D N T S P L F R T P L S F T N P L H S D D S D S D E R N S D G A V T Q N K T N I S T A S A T V S A A T S T E S I S T R K V L P M S I A R H N I A G T T H S G A E K D V D V S E D S P P P L P E R T P E S F V L A S E H N T P V R S E W S E L Q S Q E R S E Q K K S E G L I T S E N E K C D H P A G G I H Y E M C I E C P P T F S D K R E Q I S E N P T E A T D I G F G N R C G K P K G P R D P P S E W T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed:18559503). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed:17329398, PubMed:27134172). Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (PubMed:27134172).
Alternative names
Tyrosine-protein phosphatase non-receptor type 12, PTP-PEST, Protein-tyrosine phosphatase G1, PTPG1, PTPN12
Recommended products
Recombinant Human PTPN12 protein is a Human Full Length protein, in the 1 to 780 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Alternative names
Tyrosine-protein phosphatase non-receptor type 12, PTP-PEST, Protein-tyrosine phosphatase G1, PTPG1, PTPN12
Key facts
- Expression system
Wheat germ
- Tags
Tag free
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- Q05209-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M E Q V E I L R K F I Q R V Q A M K S P D H N G E D N F A R D F M R L R R L S T K Y R T E K I Y P T A T G E K E E N V K K N R Y K D I L P F D H S R V K L T L K T P S Q D S D Y I N A N F I K G V Y G P K A Y V A T Q G P L A N T V I D F W R M I W E Y N V V I I V M A C R E F E M G R K K C E R Y W P L Y G E D P I T F A P F K I S C E D E Q A R T D Y F I R T L L L E F Q N E S R R L Y Q F H Y V N W P D H D V P S S F D S I L D M I S L M R K Y Q E H E D V P I C I H C S A G C G R T G A I C A I D Y T W N L L K A G K I P E E F N V F N L I Q E M R T Q R H S A V Q T K E Q Y E L V H R A I A Q L F E K Q L Q L Y E I H G A Q K I A D G V N E I N T E N M I S S I E P E K Q D S P P P K P P R T R S C L V E G D A K E E I L Q P P E P H P V P P I L T P S P P S A F P T V T T V W Q D N D R Y H P K P V L H M V S S E Q H S A D L N R N Y S K S T E L P G K N E S T I E Q I D K K L E R N L S F E I K K V P L Q E G P K S F D G N T L L N R G H A I K I K S A S P C I A D K I S K P Q E L S S D L N V G D T S Q N S C V D C S V T Q S N K V S V T P P E E S Q N S D T P P R P D R L P L D E K G H V T W S F H G P E N A I P I P D L S E G N S S D I N Y Q T R K T V S L T P S P T T Q V E T P D L V D H D N T S P L F R T P L S F T N P L H S D D S D S D E R N S D G A V T Q N K T N I S T A S A T V S A A T S T E S I S T R K V L P M S I A R H N I A G T T H S G A E K D V D V S E D S P P P L P E R T P E S F V L A S E H N T P V R S E W S E L Q S Q E R S E Q K K S E G L I T S E N E K C D H P A G G I H Y E M C I E C P P T F S D K R E Q I S E N P T E A T D I G F G N R C G K P K G P R D P P S E W T
- Accession
- Protein length
Full Length
- Predicted molecular weight
114.5 kDa
- Amino acids
1 to 780
- Nature
Recombinant
Specifications
- Form
Liquid
General info
- Function
Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed:18559503). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed:17329398, PubMed:27134172). Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (PubMed:27134172).
- Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 4 subfamily.
- Post-translational modifications
Phosphorylated by STK24/MST3 and this results in inhibition of its activity.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
- Activity summary
The Protein Tyrosine Phosphatase Non-Receptor Type 12 (PTPN12) often referred to as PTP-PeST is an enzyme that plays a significant mechanical role within cells. PTPN12 is part of the protein tyrosine phosphatase (PTP) family which is responsible for removing phosphate groups from phosphotyrosine residues on proteins. This activity regulates many cellular processes including growth and differentiation. PTPN12 has a molecular mass of approximately 86 kDa. It is ubiquitously expressed in various tissues but shows higher expression in hematopoietic cells.
- Biological function summary
As a non-receptor type phosphatase PTPN12 partners with several proteins to perform essential cellular functions. It interacts with multiple components in the cell participating in the assembly of protein complexes that manage cytoskeletal dynamics and cell movement. PTPN12 influences signal transduction pathways by interacting with adapter proteins such as p130Cas and Paxillin which are vital for cell adhesion and migration. It also plays a role in maintaining cellular homeostasis by controlling signaling cascades that prevent excessive cell proliferation.
- Pathways
PTPN12 takes part in critical signal transduction networks most notably the Integrin signaling and RhoA pathways. In the Integrin pathway PTPN12 negatively regulates signaling by dephosphorylating focal adhesion-associated proteins. This regulation affects cell attachment to the extracellular matrix and cell motility. The interaction of PTPN12 with proteins such as Src and FAK (Focal Adhesion Kinase) illustrates its involvement in these processes. In the RhoA pathway PTPN12 influences cytoskeletal rearrangement and cell shape regulation linking to its role in cell migration.
- Associated diseases and disorders
Disruptions in PTPN12 function associate with cancer progression such as breast cancer and inflammatory disorders. In breast cancer physiological studies indicate that PTPN12 acts as a tumor suppressor. Its loss or reduced activity may lead to unregulated cell proliferation and metastasis by failing to restrain pathways involving Src and FAK. In inflammatory disorders altered activities of PTPN12 can affect immune cell function contributing to conditions like rheumatoid arthritis. The enzyme's dysfunction can amplify inflammatory signals through faults in the regulation of immune cells linking PTPN12 to increased disease susceptibility.
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1 product image
SDS-PAGE - Recombinant Human PTPN12 protein (ab132054)
12.5% SDS-PAGE analysis of ab132054 stained with Coomassie Blue.
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