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AB164258

Recombinant Human PUS1 protein (GST tag N-Terminus)

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Recombinant Human PUS1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 399 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

PP8985, PUS1, Pseudouridylate synthase 1 homolog, tRNA pseudouridine synthase 1, tRNA pseudouridine(38-40) synthase, tRNA pseudouridylate synthase I, tRNA-uridine isomerase I

1 Images
SDS-PAGE - Recombinant Human PUS1 protein (GST tag N-Terminus) (AB164258)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PUS1 protein (GST tag N-Terminus) (AB164258)

ab164258 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9Y606

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Primary Antibodies

AB203010

Anti-PUS1 antibody [EPR20181]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PUS1 antibody [EPR20181] (AB203010)

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Proteins & Peptides

AB198661

Recombinant Human WTAP protein

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AB159886

Recombinant Human ZP2 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZP2 protein (GST tag N-Terminus) (AB159886)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":399,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9Y606","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PUS1 also known as pseudouridine synthase 1 is an enzyme responsible for catalyzing the isomerization of uridine to pseudouridine in RNA molecules. This protein has a mass of approximately 50 kDa and expresses widely in both the cytoplasm and mitochondria of human cells. Pseudouridine is the most abundant RNA modification and plays an important role in RNA stability and structure. The presence of PUS1 can vary among cell types supporting its necessity in diverse cellular contexts.
Biological function summary

The modification of RNA by PUS1 improves the stability and folding of the RNA structure aiding its functionality. The enzyme is not part of a larger complex but acts independently to achieve its role in RNA modification. Pseudouridine created by PUS1 permits enhanced base stacking and hydrogen bonding which influences the translation process and the overall efficiency of protein synthesis. This modification is important for the proper functioning of tRNA and rRNA ensuring fidelity during protein translation.

Pathways

PUS1 plays an essential role in the RNA processing and modification pathways. Its activity integrates into pathways such as ribosome biogenesis and RNA quality control. Particularly it interacts with other RNA-binding proteins and enzymes like TRUB1 another pseudouridine synthase which also contributes to the maturation of structured RNAs. These pathways are vital for maintaining cellular homeostasis and responding to various physiological demands.

Mutations in PUS1 are linked to mitochondrial myopathy and sideroblastic anemia a rare condition characterized by muscle weakness and anemia. These disorders illustrate the critical impact of RNA modification defects on cellular energy production and red blood cell formation. In these conditions PUS1 may interact with defective proteins leading to impaired pseudouridylation disrupting normal cellular functions and contributing to disease pathology. Understanding the role of PUS1 might provide insights into potential therapeutic strategies for such disorders.
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Specifications

Form

Liquid

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General info

Function

Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs (PubMed : 15772074, PubMed : 24722331). Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed : 24722331). Also catalyzes pseudouridylation of mRNAs : mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed : 31477916, PubMed : 35051350). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed : 35051350). Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed : 35051350). Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA (PubMed : 24722331).

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

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Product protocols

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Target data

Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs (PubMed : 15772074, PubMed : 24722331). Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA (PubMed : 24722331). Also catalyzes pseudouridylation of mRNAs : mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed : 31477916, PubMed : 35051350). Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions (PubMed : 35051350). Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing (PubMed : 35051350). Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA (PubMed : 24722331).
See full target information PUS1
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