Recombinant Human PVRL1/NECTIN1 protein (His tag) is a Human Fragment protein, in the 1 to 334 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
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Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M A R M G L A G A A G R W W G L A L G L T A F F L P G V H S Q V V Q V N D S M Y G F I G T D V V L H C S F A N P L P S V K I T Q V T W Q K S T N G S K Q N V A I Y N P S M G V S V L A P Y R E R V E F L R P S F T D G T I R L S R L E L E D E G V Y I C E F A T F P T G N R E S Q L N L T V M A K P T N W I E G T Q A V L R A K K G Q D D K V L V A T C T S A N G K P P S V V S W E T R L K G E A E Y Q E I R N P N G T V T V I S R Y R L V P S R E A H Q Q S L A C I V N Y H M D R F K E S L T L N V Q Y E P E V T I E G F D G N W Y L Q R M D V K L T C K A D A N P P A T E Y H W T T L N G S L P K G V E A Q N R T L F F K G P I N Y S L A G T Y I C E A T N P I G T R S G Q V E V N I T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system (PubMed:21325282). Acts by forming homophilic or heterophilic trans-dimers (PubMed:21325282). Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4 (By similarity). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Involved in synaptogegesis (By similarity). Has some neurite outgrowth-promoting activity (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN3: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Required for enamel mineralization (By similarity).(Microbial infection) Acts as a receptor for herpes simplex virus 1/HHV-1, herpes simplex virus 2/HHV-2, and pseudorabies virus/PRV (PubMed:21980294, PubMed:25231300, PubMed:28381567, PubMed:28542478, PubMed:34587223, PubMed:38857290, PubMed:39048823, PubMed:39048830, PubMed:7721102, PubMed:9616127, PubMed:9657005). Constitutes the major receptor for herpes simplex virus 1/HHV-1 entry into host cells (PubMed:34587223).
Alternative names
HVEC, PRR1, PVRL1, PVRL1, PRR1, HVEC, NECTIN1, Nectin-1, Herpes virus entry mediator C, Herpesvirus Ig-like receptor, Nectin cell adhesion molecule 1, Poliovirus receptor-related protein 1, Herpesvirus entry mediator C, HveC, HIgR
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Recombinant Human PVRL1/NECTIN1 protein (His tag) is a Human Fragment protein, in the 1 to 334 aa range, expressed in HEK 293, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
>98% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
His tag C-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q15223-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 100% PBS
Sequence info
Amino acid sequence
M A R M G L A G A A G R W W G L A L G L T A F F L P G V H S Q V V Q V N D S M Y G F I G T D V V L H C S F A N P L P S V K I T Q V T W Q K S T N G S K Q N V A I Y N P S M G V S V L A P Y R E R V E F L R P S F T D G T I R L S R L E L E D E G V Y I C E F A T F P T G N R E S Q L N L T V M A K P T N W I E G T Q A V L R A K K G Q D D K V L V A T C T S A N G K P P S V V S W E T R L K G E A E Y Q E I R N P N G T V T V I S R Y R L V P S R E A H Q Q S L A C I V N Y H M D R F K E S L T L N V Q Y E P E V T I E G F D G N W Y L Q R M D V K L T C K A D A N P P A T E Y H W T T L N G S L P K G V E A Q N R T L F F K G P I N Y S L A G T Y I C E A T N P I G T R S G Q V E V N I T
- Accession
- Protein length
Fragment
- Predicted molecular weight
35.4 kDa
- Amino acids
1 to 334
- Nature
Recombinant
- Tags
His tag C-Terminus
Specifications
- Form
Lyophilized
General info
- Function
Cell adhesion molecule that promotes cell-cell contacts and plays important roles in the development of the nervous system (PubMed:21325282). Acts by forming homophilic or heterophilic trans-dimers (PubMed:21325282). Heterophilic interactions have been detected between NECTIN1 and NECTIN3 and between NECTIN1 and NECTIN4 (By similarity). Involved in axon guidance by promoting contacts between the commissural axons and the floor plate cells (By similarity). Involved in synaptogegesis (By similarity). Has some neurite outgrowth-promoting activity (By similarity). Promotes formation of checkerboard-like cellular pattern of hair cells and supporting cells in the auditory epithelium via heterophilic interaction with NECTIN3: NECTIN1 is present in the membrane of hair cells and associates with NECTIN3 on supporting cells, thereby mediating heterotypic adhesion between these two cell types (By similarity). Required for enamel mineralization (By similarity).
- Sequence similarities
Belongs to the nectin family.
- Post-translational modifications
(Microbial infection) Ubiquitinated by CBL following infection by herpes simplex virus 1/HHV-1 and association with HHV-1 envelope glycoprotein D, leading to its removal from cell surface.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PVRL1 also known as NECTIN1 or nectin-1 functions mechanically as an immunoglobulin-like cell adhesion molecule. It plays a role in creating adherens junctions between cells operating as both a homophilic and heterophilic binding partner. Nectin-1 has a molecular mass of approximately 97 kDa. It is expressed prominently in epithelial tissues and particularly in the nervous system which suggests it plays an important role in cell-cell communication in these areas.
- Biological function summary
Nectin-1 facilitates cellular processes through its involvement in cell adhesion complexes. It functions as part of a larger complex including proteins like afadin which links to the actin cytoskeleton and promotes cell junction integrity. This organization impacts cellular adhesion migration and proliferation. Nectin-1's adhesive functions are fundamental in maintaining structural tissues and transmitting signals within cells a necessity for development and tissue maintenance.
- Pathways
Nectin-1 contributes to the activation of the MAPK/ERK signaling pathway central to regulating various cell functions including proliferation and differentiation. It also participates in the cadherin-catenin complex pathway working in conjunction with E-cadherin a critical protein in maintaining epithelial cell layer integrity. These pathways highlight nectin-1’s role as a signal transducer affecting how cells interact and react to their environment.
- Associated diseases and disorders
Nectin-1 is implicated in cleft lip and palate disorder. Mutations affecting nectin-1 can disrupt cell adhesion processes necessary for proper development of facial structures. Furthermore alterations in nectin-1 gene expression are associated with susceptibility to herpes simplex virus infections as nectin-1 acts as a viral entry receptor. In these conditions its interaction with closely related membrane proteins such as nectin-3 is significant in disease manifestation and progression.
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SDS-PAGE - Recombinant Human PVRL1/NECTIN1 protein (His tag) (ab276411)
SDS-PAGE analysis of ab276411
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