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AB162262

Recombinant Human PYCR2 protein (GST tag N-Terminus)

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Recombinant Human PYCR2 protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 320 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Pyrroline-5-carboxylate reductase 2, P5C reductase 2, P5CR 2, PYCR2

1 Images
SDS-PAGE - Recombinant Human PYCR2 protein (GST tag N-Terminus) (AB162262)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PYCR2 protein (GST tag N-Terminus) (AB162262)

ab162262 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q96C36

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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SDS-PAGE - Recombinant Human TWF1/Twinfilin-1 protein (AB131676)

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Primary Antibodies

AB103535

Anti-PYCR2 antibody

Western blot - Anti-PYCR2 antibody (AB103535)

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Proteins & Peptides

AB166176

Recombinant Human WDR62 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human WDR62 protein (GST tag N-Terminus) (AB166176)

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Proteins & Peptides

AB116154

Recombinant Human PSMA8 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PSMA8 protein (His tag N-Terminus) (AB116154)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MSVGFIGAGQLAYALARGFTAAGILSAHKIIASSPEMNLPTVSALRKMGVNLTRSNKETVKHSDVLFLAVKPHIIPFILDEIGADVQARHIVVSCAAGVTISSVEKKLMAFQPAPKVIRCMTNTPVVVQEGATVYATGTHALVEDGQLLEQLMSSVGFCTEVEEDLIDAVTGLSGSGPAYAFMALDALADGGVKMGLPRRLAIQLGAQALLGAAKMLLDSEQHPCQLKDNVCSPGGATIHALHFLESGGFRSLLINAVEASCIRTRELQSMADQEKISPAALKKTLLDRVKLESPTVSTLTPSSPGKLLTRSLALGGKKD","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":320,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q96C36","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCR2 also known as Pyrroline-5-carboxylate reductase 2 is an enzyme involved in proline biosynthesis. It has a molecular mass of approximately 31 kDa. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline an amino acid important for protein synthesis and cellular stress responses. Expressed broadly in human tissues PYCR2 shows notable expression in the liver brain and various muscle tissues indicating its wide functional relevance across different physiological systems.
Biological function summary

The enzyme participates in maintaining proline levels and contributes to cellular osmotic balance and protection against oxidative stress. It is not typically part of a larger protein complex but interacts with other metabolic enzymes that support similar stress response pathways. By catalyzing an important step in the proline biosynthesis pathway PYCR2 helps sustain cellular redox states and protein turnover rates under stressful conditions.

Pathways

PYCR2 plays a significant role in the proline metabolism pathway and glutamate pathway. The functioning of this enzyme in these pathways supports the synthesis and recycling of proline which is important for cell survival under osmotic and oxidative stress conditions. The enzyme cooperates indirectly with proteins such as glutamate dehydrogenase facilitating the metabolic flux through interconnected pathways that manage cellular nitrogen balance.

Mutations in PYCR2 have been associated with conditions such as Hypomyelinating Leukodystrophy 10 and Proline Metabolism Disorder. These conditions often showcase symptoms related to central nervous system abnormalities and metabolic imbalances. The disruptions in PYCR2 activity may also alter interactions with proteins involved in these pathways such as Δ1-Pyrroline-5-carboxylate synthase thereby exacerbating the disease states.
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Specifications

Form

Liquid

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General info

Function

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). Involved in cellular response to oxidative stress (PubMed : 25865492). In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+) (PubMed : 2722838, PubMed : 6894153).

Sequence similarities

Belongs to the pyrroline-5-carboxylate reductase family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). At physiologic concentrations, has higher specific activity in the presence of NADH (PubMed : 23024808, PubMed : 2722838, PubMed : 6894153). Involved in cellular response to oxidative stress (PubMed : 25865492). In some cell types, such as erythrocytes, its primary function may be the generation of NADP(+) (PubMed : 2722838, PubMed : 6894153).
See full target information PYCR2
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