JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB139626

Recombinant Human PYCRL protein (His tag N-Terminus)

5

(1 Review)

|

(0 Publication)

Recombinant Human PYCRL protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 274 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

PYCRL, PYCR3, Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase-like protein

2 Images
SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)

SDS-PAGE analysis of ab139626

SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human PYCRL protein (His tag N-Terminus) (AB139626)

15% SDS-PAGE analysis of ab139626 (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q53H96

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK","proteinLength":"Full Length","predictedMolecularWeight":"31 kDa","actualMolecularWeight":null,"aminoAcidEnd":274,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q53H96","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PYCRL also known as Pyrroline-5-carboxylate reductase 3 is an enzyme involved in proline biosynthesis. It catalyzes the conversion of pyrroline-5-carboxylate to proline. This protein has a molecular mass of approximately 29 kDa. It is widely expressed in various tissues including the liver kidney and placenta. Its activity plays an important role in maintaining proline homeostasis within cells.
Biological function summary

PYCRL contributes to the cellular process of amino acid metabolism. It participates in a network of enzymatic reactions that balance the levels of proline an amino acid important for protein synthesis and cellular response to osmotic stress. PYCRL forms part of a proline metabolic pathway but does not operate as part of a larger protein complex. By converting pyrroline-5-carboxylate to proline it helps in collagen synthesis and other proline-related functions within the body.

Pathways

PYCRL functionally integrates into the proline biosynthesis pathway. This pathway is essential for amino acid metabolism and stress response. PYCRL works alongside proteins such as ALDH18A1 which synthesizes pyrroline-5-carboxylate from glutamate. The proline pathway not only supports protein production but also impacts cellular stress responses by maintaining proline as an osmoprotectant.

Proline metabolism dysregulation involving PYCRL has links to certain health conditions. Alterations in this pathway can contribute to hyperprolinemia a metabolic disorder characterized by elevated proline levels. Hyperprolinemia is related to symptoms affecting neurological development. Variations in PYCRL activity might also impact connective tissue disorders due to its role in collagen production. The enzyme is potentially connected with ALDH18A1 through their shared involvement in proline metabolism influencing disease expression.
style
left-column

Specifications

Form

Liquid

Additional notes

ab139626 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine; both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline.

Sequence similarities

Belongs to the pyrroline-5-carboxylate reductase family.

style
left-column

Product protocols

style
left-column

Target data

Enzyme that catalyzes the last step in proline biosynthesis. Proline is synthesized from either glutamate or ornithine; both are converted to pyrroline-5-carboxylate (P5C), and then to proline via pyrroline-5-carboxylate reductases (PYCRs). PYCRL is exclusively linked to the conversion of ornithine to proline.
See full target information PYCR3
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB139615

Recombinant human STK36 protein

Functional Studies - Recombinant human STK36 protein (AB139615)

  • 0
  • 0
Proteins & Peptides

AB131684

Recombinant Human FTSJ2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human FTSJ2 protein (His tag N-Terminus) (AB131684)

  • 0
  • 0
Proteins & Peptides

AB137142

Recombinant Human Cdk5 protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cdk5 protein (denatured) (His tag N-Terminus) (AB137142)

  • 0
  • 0
Proteins & Peptides

AB131673

Recombinant Human MED7 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MED7 protein (His tag N-Terminus) (AB131673)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com