Recombinant Human PYGL protein is a Human Full Length protein, in the 1 to 847 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
>80% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
M G S S H H H H H H S S G L V P R G S H M G S E F E L R R Q A S M A K P L T D Q E K R R Q I S I R G I V G V E N V A E L K K S F N R H L H F T L V K D R N V A T T R D Y Y F A L A H T V R D H L V G R W I R T Q Q H Y Y D K C P K R V Y Y L S L E F Y M G R T L Q N T M I N L G L Q N A C D E A I Y Q L G L D I E E L E E I E E D A G L G N G G L G R L A A C F L D S M A T L G L A A Y G Y G I R Y E Y G I F N Q K I R D G W Q V E E A D D W L R Y G N P W E K S R P E F M L P V H F Y G K V E H T N T G T K W I D T Q V V L A L P Y D T P V P G Y M N N T V N T M R L W S A R A P N D F N L R D F N V G D Y I Q A V L D R N L A E N I S R V L Y P N D N F F E G K E L R L K Q E Y F V V A A T L Q D I I R R F K A S K F G S T R G A G T V F D A F P D Q V A I Q L N D T H P A L A I P E L M R I F V D I E K L P W S K A W E L T Q K T F A Y T N H T V L P E A L E R W P V D L V E K L L P R H L E I I Y E I N Q K H L D R I V A L F P K D V D R L R R M S L I E E E G S K R I N M A H L C I V G S H A V N G V A K I H S D I V K T K V F K D F S E L E P D K F Q N K T N G I T P R R W L L L C N P G L A E L I A E K I G E D Y V K D L S Q L T K L H S F L G D D V F L R E L A K V K Q E N K L K F S Q F L E T E Y K V K I N P S S M F D V Q V K R I H E Y K R Q L L N C L H V I T M Y N R I K K D P K K L F V P R T V I I G G K A A P G Y H M A K M I I K L I T S V A D V V N N D P M V G S K L K V I F L E N Y R V S L A E K V I P A T D L S E Q I S T A G T E A S G T G N M K F M L N G A L T I G T M D G A N V E M A E E A G E E N L F I F G M R I D D V A A L D K K G Y E A K E Y Y E A L P E L K L V I D Q I D N G F F S P K Q P D L F K D I I N M L F Y H D R F K V F A D Y E A Y V K C Q D K V S Q L Y M N P K A W N M V L K N I A A S G K F S S D R T I K E Y A Q N I W N V E P S D L K I S L S N E S N K V N G N
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
PYGL
Recombinant Human PYGL protein is a Human Full Length protein, in the 1 to 847 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.
>80% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
No
Human
pH: 7.4
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
M G S S H H H H H H S S G L V P R G S H M G S E F E L R R Q A S M A K P L T D Q E K R R Q I S I R G I V G V E N V A E L K K S F N R H L H F T L V K D R N V A T T R D Y Y F A L A H T V R D H L V G R W I R T Q Q H Y Y D K C P K R V Y Y L S L E F Y M G R T L Q N T M I N L G L Q N A C D E A I Y Q L G L D I E E L E E I E E D A G L G N G G L G R L A A C F L D S M A T L G L A A Y G Y G I R Y E Y G I F N Q K I R D G W Q V E E A D D W L R Y G N P W E K S R P E F M L P V H F Y G K V E H T N T G T K W I D T Q V V L A L P Y D T P V P G Y M N N T V N T M R L W S A R A P N D F N L R D F N V G D Y I Q A V L D R N L A E N I S R V L Y P N D N F F E G K E L R L K Q E Y F V V A A T L Q D I I R R F K A S K F G S T R G A G T V F D A F P D Q V A I Q L N D T H P A L A I P E L M R I F V D I E K L P W S K A W E L T Q K T F A Y T N H T V L P E A L E R W P V D L V E K L L P R H L E I I Y E I N Q K H L D R I V A L F P K D V D R L R R M S L I E E E G S K R I N M A H L C I V G S H A V N G V A K I H S D I V K T K V F K D F S E L E P D K F Q N K T N G I T P R R W L L L C N P G L A E L I A E K I G E D Y V K D L S Q L T K L H S F L G D D V F L R E L A K V K Q E N K L K F S Q F L E T E Y K V K I N P S S M F D V Q V K R I H E Y K R Q L L N C L H V I T M Y N R I K K D P K K L F V P R T V I I G G K A A P G Y H M A K M I I K L I T S V A D V V N N D P M V G S K L K V I F L E N Y R V S L A E K V I P A T D L S E Q I S T A G T E A S G T G N M K F M L N G A L T I G T M D G A N V E M A E E A G E E N L F I F G M R I D D V A A L D K K G Y E A K E Y Y E A L P E L K L V I D Q I D N G F F S P K Q P D L F K D I I N M L F Y H D R F K V F A D Y E A Y V K C Q D K V S Q L Y M N P K A W N M V L K N I A A S G K F S S D R T I K E Y A Q N I W N V E P S D L K I S L S N E S N K V N G N
Full Length
100.7 kDa
1 to 847
Recombinant
His tag N-Terminus
Liquid
ab202149 was purified using conventional chromatography techniques.
Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
Belongs to the glycogen phosphorylase family.
Acetylation, which is up-regulated by glucose and insulin and down-regulated by glucagon, inhibits the glycogen phosphorylase activity by promoting PPP1R3B-mediated recruitment of phosphatase PP1 and Ser-15 dephosphorylation.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
PYGL also known as glycogen phosphorylase liver form is an enzyme with a monomeric mass of around 97 kDa. Mechanically PYGL catalyzes the breakdown of glycogen into glucose-1-phosphate by cleaving alpha-14-glycosidic bonds. This enzyme primarily operates in the liver where it plays a critical role in regulating glucose release into the bloodstream. PYGL's activity is tightly controlled by hormonal signals which ensure proper glucose levels.
The enzyme plays a major role in maintaining energy homeostasis by mobilizing glucose from glycogen stores during fasting states. It does not function as part of a larger complex operating instead as a homodimer when activated. PYGL is critical for the liver's ability to supply glucose to other organs when dietary glucose is not available. Proper functioning of PYGL helps maintain a balance between glucose storage and release contributing to overall metabolic equilibrium.
PYGL has a notable role in glycogenolysis a process that liberates stored glycogen as glucose-1-phosphate. This enzyme also connects with gluconeogenesis where it impacts the production of glucose from non-carbohydrate sources. In these pathways its activity often intersects with other enzymes namely phosphoglucomutase and glucose-6-phosphatase which further process the products of PYGL’s activity.
PYGL is associated with conditions such as Hers disease a glycogen storage disease caused by a deficiency in hepatic glycogen phosphorylase. This leads to excessive glycogen accumulation in the liver and resultant hypoglycemia. Additionally abnormal PYGL activity relates to type 2 diabetes where altered glycogen metabolism impacts insulin response and glucose homeostasis. In these contexts it maintains functional connections with proteins like insulin receptor and glycogen synthase which together influence carbohydrate metabolism balance.
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15% SDS-PAGE analysis of ab202149 (3 μg).
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