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Recombinant Human PYGL protein is a Human Full Length protein, in the 1 to 847 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.

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Images

SDS-PAGE - Recombinant Human PYGL protein (AB202149), expandable thumbnail

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S E F E L R R Q A S M A K P L T D Q E K R R Q I S I R G I V G V E N V A E L K K S F N R H L H F T L V K D R N V A T T R D Y Y F A L A H T V R D H L V G R W I R T Q Q H Y Y D K C P K R V Y Y L S L E F Y M G R T L Q N T M I N L G L Q N A C D E A I Y Q L G L D I E E L E E I E E D A G L G N G G L G R L A A C F L D S M A T L G L A A Y G Y G I R Y E Y G I F N Q K I R D G W Q V E E A D D W L R Y G N P W E K S R P E F M L P V H F Y G K V E H T N T G T K W I D T Q V V L A L P Y D T P V P G Y M N N T V N T M R L W S A R A P N D F N L R D F N V G D Y I Q A V L D R N L A E N I S R V L Y P N D N F F E G K E L R L K Q E Y F V V A A T L Q D I I R R F K A S K F G S T R G A G T V F D A F P D Q V A I Q L N D T H P A L A I P E L M R I F V D I E K L P W S K A W E L T Q K T F A Y T N H T V L P E A L E R W P V D L V E K L L P R H L E I I Y E I N Q K H L D R I V A L F P K D V D R L R R M S L I E E E G S K R I N M A H L C I V G S H A V N G V A K I H S D I V K T K V F K D F S E L E P D K F Q N K T N G I T P R R W L L L C N P G L A E L I A E K I G E D Y V K D L S Q L T K L H S F L G D D V F L R E L A K V K Q E N K L K F S Q F L E T E Y K V K I N P S S M F D V Q V K R I H E Y K R Q L L N C L H V I T M Y N R I K K D P K K L F V P R T V I I G G K A A P G Y H M A K M I I K L I T S V A D V V N N D P M V G S K L K V I F L E N Y R V S L A E K V I P A T D L S E Q I S T A G T E A S G T G N M K F M L N G A L T I G T M D G A N V E M A E E A G E E N L F I F G M R I D D V A A L D K K G Y E A K E Y Y E A L P E L K L V I D Q I D N G F F S P K Q P D L F K D I I N M L F Y H D R F K V F A D Y E A Y V K C Q D K V S Q L Y M N P K A W N M V L K N I A A S G K F S S D R T I K E Y A Q N I W N V E P S D L K I S L S N E S N K V N G N

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.

Alternative names

Recommended products

Recombinant Human PYGL protein is a Human Full Length protein, in the 1 to 847 aa range, expressed in Escherichia coli, with >80% purity and suitable for SDS-PAGE.

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Accession
P06737-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.4
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M G S S H H H H H H S S G L V P R G S H M G S E F E L R R Q A S M A K P L T D Q E K R R Q I S I R G I V G V E N V A E L K K S F N R H L H F T L V K D R N V A T T R D Y Y F A L A H T V R D H L V G R W I R T Q Q H Y Y D K C P K R V Y Y L S L E F Y M G R T L Q N T M I N L G L Q N A C D E A I Y Q L G L D I E E L E E I E E D A G L G N G G L G R L A A C F L D S M A T L G L A A Y G Y G I R Y E Y G I F N Q K I R D G W Q V E E A D D W L R Y G N P W E K S R P E F M L P V H F Y G K V E H T N T G T K W I D T Q V V L A L P Y D T P V P G Y M N N T V N T M R L W S A R A P N D F N L R D F N V G D Y I Q A V L D R N L A E N I S R V L Y P N D N F F E G K E L R L K Q E Y F V V A A T L Q D I I R R F K A S K F G S T R G A G T V F D A F P D Q V A I Q L N D T H P A L A I P E L M R I F V D I E K L P W S K A W E L T Q K T F A Y T N H T V L P E A L E R W P V D L V E K L L P R H L E I I Y E I N Q K H L D R I V A L F P K D V D R L R R M S L I E E E G S K R I N M A H L C I V G S H A V N G V A K I H S D I V K T K V F K D F S E L E P D K F Q N K T N G I T P R R W L L L C N P G L A E L I A E K I G E D Y V K D L S Q L T K L H S F L G D D V F L R E L A K V K Q E N K L K F S Q F L E T E Y K V K I N P S S M F D V Q V K R I H E Y K R Q L L N C L H V I T M Y N R I K K D P K K L F V P R T V I I G G K A A P G Y H M A K M I I K L I T S V A D V V N N D P M V G S K L K V I F L E N Y R V S L A E K V I P A T D L S E Q I S T A G T E A S G T G N M K F M L N G A L T I G T M D G A N V E M A E E A G E E N L F I F G M R I D D V A A L D K K G Y E A K E Y Y E A L P E L K L V I D Q I D N G F F S P K Q P D L F K D I I N M L F Y H D R F K V F A D Y E A Y V K C Q D K V S Q L Y M N P K A W N M V L K N I A A S G K F S S D R T I K E Y A Q N I W N V E P S D L K I S L S N E S N K V N G N

Accession

P06737

Protein length

Full Length

Predicted molecular weight

100.7 kDa

Amino acids

1 to 847

Nature

Recombinant

Tags

His tag N-Terminus

Specifications

Form

Liquid

Additional notes

ab202149 was purified using conventional chromatography techniques.

General info

Function

Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.

Sequence similarities

Belongs to the glycogen phosphorylase family.

Post-translational modifications

Acetylation, which is up-regulated by glucose and insulin and down-regulated by glucagon, inhibits the glycogen phosphorylase activity by promoting PPP1R3B-mediated recruitment of phosphatase PP1 and Ser-15 dephosphorylation.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PYGL also known as glycogen phosphorylase liver form is an enzyme with a monomeric mass of around 97 kDa. Mechanically PYGL catalyzes the breakdown of glycogen into glucose-1-phosphate by cleaving alpha-14-glycosidic bonds. This enzyme primarily operates in the liver where it plays a critical role in regulating glucose release into the bloodstream. PYGL's activity is tightly controlled by hormonal signals which ensure proper glucose levels.

Biological function summary

The enzyme plays a major role in maintaining energy homeostasis by mobilizing glucose from glycogen stores during fasting states. It does not function as part of a larger complex operating instead as a homodimer when activated. PYGL is critical for the liver's ability to supply glucose to other organs when dietary glucose is not available. Proper functioning of PYGL helps maintain a balance between glucose storage and release contributing to overall metabolic equilibrium.

Pathways

PYGL has a notable role in glycogenolysis a process that liberates stored glycogen as glucose-1-phosphate. This enzyme also connects with gluconeogenesis where it impacts the production of glucose from non-carbohydrate sources. In these pathways its activity often intersects with other enzymes namely phosphoglucomutase and glucose-6-phosphatase which further process the products of PYGL’s activity.

Associated diseases and disorders

PYGL is associated with conditions such as Hers disease a glycogen storage disease caused by a deficiency in hepatic glycogen phosphorylase. This leads to excessive glycogen accumulation in the liver and resultant hypoglycemia. Additionally abnormal PYGL activity relates to type 2 diabetes where altered glycogen metabolism impacts insulin response and glucose homeostasis. In these contexts it maintains functional connections with proteins like insulin receptor and glycogen synthase which together influence carbohydrate metabolism balance.

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1 product image

  • SDS-PAGE - Recombinant Human PYGL protein (ab202149), expandable thumbnail

    SDS-PAGE - Recombinant Human PYGL protein (ab202149)

    15% SDS-PAGE analysis of ab202149 (3 μg).

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Product protocols

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