Recombinant Human Pyrophosphatase 1 protein is a Human Full Length protein, in the 1 to 289 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M S G F S T E E R A A P F S L E Y R V F L K N E K G Q Y I S P F H D I P I Y A D K D V F H M V V E V P R W S N A K M E I A T K D P L N P I K Q D V K K G K L R Y V A N L F P Y K G Y I W N Y G A I P Q T W E D P G H N D K H T G C C G D N D P I D V C E I G S K V C A R G E I I G V K V L G I L A M I D E G E T D W K V I A I N V D D P D A A N Y N D I N D V K R L K P G Y L E A T V D W F R R Y K V P D G K P E N E F A F N A E F K D K D F A I D I I K S T H D H W K A L V T K K T N G K G I S C M N T T L S E S P F K C D P D A A R A I V D A L P P P C E S A C T V P T D V D K W F H H Q K N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
IOPPP, PP, PPA1, Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, PPase
Recommended products
Recombinant Human Pyrophosphatase 1 protein is a Human Full Length protein, in the 1 to 289 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q15181-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M S G F S T E E R A A P F S L E Y R V F L K N E K G Q Y I S P F H D I P I Y A D K D V F H M V V E V P R W S N A K M E I A T K D P L N P I K Q D V K K G K L R Y V A N L F P Y K G Y I W N Y G A I P Q T W E D P G H N D K H T G C C G D N D P I D V C E I G S K V C A R G E I I G V K V L G I L A M I D E G E T D W K V I A I N V D D P D A A N Y N D I N D V K R L K P G Y L E A T V D W F R R Y K V P D G K P E N E F A F N A E F K D K D F A I D I I K S T H D H W K A L V T K K T N G K G I S C M N T T L S E S P F K C D P D A A R A I V D A L P P P C E S A C T V P T D V D K W F H H Q K N
- Accession
- Q15181
- Protein length
- Full Length
- Predicted molecular weight
- 35.2 kDa
- Amino acids
- 1 to 289
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab123186 is purified using conventional chromatography techniques.
General info
- Sequence similarities
Belongs to the PPase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pyrophosphatase 1 also known as iPPase or inorganic pyrophosphatase 1 is an enzyme responsible for the hydrolysis of inorganic pyrophosphate (PPi) into two phosphate ions. This reaction is energetically favorable and provides the driving force for many biosynthetic processes. Pyrophosphatase 1 is a soluble cytosolic enzyme with a molecular mass of about 33 kDa. It is expressed in various tissues especially high in metabolically active cells like those found in the liver and kidney.
- Biological function summary
Pyrophosphate hydrolysis by pyrophosphatase 1 plays a critical role in energy metabolism and phosphate recycling within cells. This enzyme functions independently and is not part of a larger complex. Its activity maintains the balance of phosphate compounds in the cytosol. This balance is important for cellular functions like DNA and RNA synthesis where the removal of PPi is necessary to drive these reactions forward.
- Pathways
Pyrophosphatase 1 influences several key metabolic routes such as nucleotide and protein biosynthesis. The enzyme ensures the continuation of these pathways by keeping PPi concentrations low which is important for reactions like aminoacyl-tRNA synthetase activity. Pyrophosphatase 1 connects with enzymes like polymerases and ligases through these pathways facilitating a range of anabolic processes by removing inhibitory PPi byproducts.
- Associated diseases and disorders
The dysregulation of pyrophosphatase 1 can impact conditions like hypophosphatasia and some forms of arthritis. In hypophosphatasia defective function of alkaline phosphatase leads to inadequate pyrophosphate breakdown affecting bone mineralization. Pyrophosphatase 1 by regulating PPi indirectly influences the symptoms when phosphate balance is disrupted. Furthermore its role in phosphate metabolism associates pyrophosphatase 1 with pathways involving matrix proteins that are implicated in mineralization disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Pyrophosphatase 1 protein (ab123186)
15% SDS-PAGE showing ab123186 (3μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com