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AB100986

Recombinant Human QPRT protein (His tag N-Terminus)

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Recombinant Human QPRT protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 297 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

Nicotinate-nucleotide pyrophosphorylase [carboxylating], Quinolinate phosphoribosyltransferase [decarboxylating], QAPRTase, QPRTase, QPRT

1 Images
SDS-PAGE - Recombinant Human QPRT protein (His tag N-Terminus) (AB100986)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human QPRT protein (His tag N-Terminus) (AB100986)

ab100986 at 3 μg being analysed by 15% SDS PAGE.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q15274

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMDAEGLALLLPPVTLAALVDSWLREDCPGLNYAALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFLPEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKLFAKEVAPVPKIH","proteinLength":"Full Length","predictedMolecularWeight":"32.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":297,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15274","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

QPRT also known as Nicotinate-nucleotide pyrophosphorylase [carboxylating] or Quinolinate phosphoribosyltransferase functions as an important enzyme in the kynurenine pathway a metabolic pathway leading to the production of nicotinamide adenine dinucleotide (NAD+). The enzyme catalyzes the conversion of quinolinate to nicotinic acid mononucleotide. QPRT has a molecular mass of approximately 34 kDa. It is expressed in various tissues most notably in the liver kidney and brain.
Biological function summary

QPRT influences the synthesis of NAD+ which is essential for cellular redox reactions and energy metabolism. The enzyme operates as a monomer and does not typically form part of a larger complex. However its activity is important in maintaining NAD+ levels which can impact a wide range of cellular processes such as DNA repair signal transduction and metabolism.

Pathways

QPRT holds a significant role in the kynurenine pathway and NAD+ biosynthesis. This pathway includes several enzymes such as kynurenine 3-monooxygenase and kynureninase which collaboratively contribute to the degradation of tryptophan ultimately affecting the levels of important coenzymes and neurotransmitters. The NAD+ biosynthesis pathway involving QPRT also interacts with pathways for ATP generation connecting with other metabolic processes.

QPRT's malfunction links to neurological disorders such as Alzheimer's disease due to its role in NAD+ metabolism and potential effects on neuronal health and energy supply. Additionally alterations in QPRT activity have associations with liver diseases considering its expression and function in metabolic pathways within hepatic tissues. Interaction with proteins like kynurenine aminotransferase highlights its involvement in maintaining metabolic balance within these conditions.
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Specifications

Form

Liquid

Additional notes

purified by using conventional chromatography techniques.

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General info

Function

Involved in the catabolism of quinolinic acid (QA).

Sequence similarities

Belongs to the NadC/ModD family.

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Product protocols

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Target data

Involved in the catabolism of quinolinic acid (QA).
See full target information Nicotinate-nucleotide pyrophosphorylase [carboxylating]
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Complementary Products

Primary Antibodies

AB171944

Anti-QPRT antibody [EPR11941(B)]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-QPRT antibody [EPR11941(B)] (AB171944)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150121

Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgM mu chain (Alexa Fluor® 488) (AB150121)

  • 5
  • 5
Assay Kits

AB284020

Human QPRT ELISA Kit

Sandwich ELISA - Human QPRT ELISA Kit (AB284020)

  • 0
  • 0

Product promise

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