Recombinant Human Quinone oxidoreductase protein - BSA and Azide free is a Human Full Length protein, in the 1 to 329 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A T G Q K L M R A V R V F E F G G P E V L K L R S D I A V P I P K D H Q V L I K V H A C G V N P V E T Y I R S G T Y S R K P L L P Y T P G S D V A G V I E A V G D N A S A F K K G D R V F T S S T I S G G Y A E Y A L A A D H T V Y K L P E K L D F K Q G A A I G I P Y F T A Y R A L I H S A C V K A G E S V L V H G S G G V G L A A C Q I A R A Y G L K I L G T A G T E E G Q K I V L Q N G A H E V F N H R E V N Y I D K I K K Y V G E K G I D I I I E M L A N V N L S K D L S L L S H G G R V I V V G S R G T I E I N P R D T M A K E S S I I G V T L F S S T K E E F Q Q Y A A A L Q A G M E I G W L K P V I G S Q Y P L E K V A E A H E N I I H G S G A T G K M I L L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
Alternative names
Quinone oxidoreductase, NADPH:quinone reductase, Zeta-crystallin, CRYZ
Recommended products
Recombinant Human Quinone oxidoreductase protein - BSA and Azide free is a Human Full Length protein, in the 1 to 329 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q08257-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M A T G Q K L M R A V R V F E F G G P E V L K L R S D I A V P I P K D H Q V L I K V H A C G V N P V E T Y I R S G T Y S R K P L L P Y T P G S D V A G V I E A V G D N A S A F K K G D R V F T S S T I S G G Y A E Y A L A A D H T V Y K L P E K L D F K Q G A A I G I P Y F T A Y R A L I H S A C V K A G E S V L V H G S G G V G L A A C Q I A R A Y G L K I L G T A G T E E G Q K I V L Q N G A H E V F N H R E V N Y I D K I K K Y V G E K G I D I I I E M L A N V N L S K D L S L L S H G G R V I V V G S R G T I E I N P R D T M A K E S S I I G V T L F S S T K E E F Q Q Y A A A L Q A G M E I G W L K P V I G S Q Y P L E K V A E A H E N I I H G S G A T G K M I L L L
- Accession
- Q08257
- Protein length
- Full Length
- Predicted molecular weight
- 37.6 kDa
- Amino acids
- 1 to 329
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab180303 was purified by using conventional chromatography techniques.
General info
- Function
Does not have alcohol dehydrogenase activity. Binds NADP and acts through a one-electron transfer process. Orthoquinones, such as 1,2-naphthoquinone or 9,10-phenanthrenequinone, are the best substrates (in vitro). May act in the detoxification of xenobiotics. Interacts with (AU)-rich elements (ARE) in the 3'-UTR of target mRNA species. Enhances the stability of mRNA coding for BCL2. NADPH binding interferes with mRNA binding.
- Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as CRYZ
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Quinone oxidoreductase also known by its popular alternate name NQO1 is a flavoenzyme with a molecular mass of approximately 31 kDa. It functions mechanically by catalyzing the reduction of quinones to hydroquinones utilizing NAD(P)H as an electron donor. This enzymatic activity helps in preventing redox cycling and oxidative stress by removing reactive quinones. Quinone oxidoreductase is expressed in various tissues including liver lung and heart highlighting its role in many biological processes.
- Biological function summary
Quinone oxidoreductase acts as a detoxifying enzyme. It maintains cellular protection against oxidative damage by neutralizing harmful quinones. This enzyme is not reported as part of a larger protein complex but it plays a complementary role with other phase II detoxification enzymes like glutathione S-transferase. Its protective attributes attribute to cell survival especially under oxidative stress conditions.
- Pathways
Quinone oxidoreductase is integral to the oxidative stress response and xenobiotic metabolism pathways. It is closely related to proteins such as cytochrome P450 and glutathione which work together to maintain cellular redox homeostasis and metabolism of foreign compounds. This collaboration allows quinone oxidoreductase to help in converting toxic substances into less harmful forms.
- Associated diseases and disorders
Quinone oxidoreductase is implicated in cancer and neurodegenerative disorders. In cancer its overexpression can lead to chemoresistance as its detoxifying functions may neutralize chemotherapeutic agents sharing functional pathways with proteins like p53. In neurodegenerative diseases altered activity of quinone oxidoreductase influences oxidative damage a common cause in disorders such as Parkinson's disease. Its relation with proteins like PARK7 (DJ-1) highlights its role in protecting neuronal cells from oxidative stress.
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1 product image
SDS-PAGE - Recombinant Human Quinone oxidoreductase protein - BSA and Azide free (ab180303)
15% SDS-PAGE analysis of ab180303 (3 μg).
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