Recombinant Human RAB13 protein is a Human Full Length protein, in the 1 to 200 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M A K A Y D H L F K L L L I G D S G V G K T C L I I R F A E D N F N N T Y I S T I G I D F K I R T V D I E G K K I K L Q V W D T A G Q E R F K T I T T A Y Y R G A M G I I L V Y D I T D E K S F E N I Q N W M K S I K E N A S A G V E R L L L G N K C D M E A K R K V Q K E Q A D K L A R E H G I R F F E T S A K S S M N V D E A F S S L A R D I L L K S G G R R S G N G N K P P S T D L K T C D K K N T N K C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.
Alternative names
GIG4, RAB13, Ras-related protein Rab-13, Cell growth-inhibiting gene 4 protein
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Recombinant Human RAB13 protein is a Human Full Length protein, in the 1 to 200 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P51153-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M A K A Y D H L F K L L L I G D S G V G K T C L I I R F A E D N F N N T Y I S T I G I D F K I R T V D I E G K K I K L Q V W D T A G Q E R F K T I T T A Y Y R G A M G I I L V Y D I T D E K S F E N I Q N W M K S I K E N A S A G V E R L L L G N K C D M E A K R K V Q K E Q A D K L A R E H G I R F F E T S A K S S M N V D E A F S S L A R D I L L K S G G R R S G N G N K P P S T D L K T C D K K N T N K C
- Accession
- P51153
- Protein length
- Full Length
- Predicted molecular weight
- 24.7 kDa
- Amino acids
- 1 to 200
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab109958 was purified using conventional chromatography techniques.
General info
- Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.
- Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
- Post-translational modifications
(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.
- Subcellular localisation
- Recycling endosome membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB13 also known by the names YPT13 and RAB11FIP5 is a small GTPase from the RAB family with a molecular mass of approximately 23 kDa. It localizes to both the cytosol and various membrane compartments. Expressed broadly in different tissues including epithelial tissues and fibroblasts RAB13 plays an active role in vesicle trafficking processes. It primarily modulates the dynamics of vesicle formation transportation and fusion making it critical for maintaining cellular membrane identity and homeostasis.
- Biological function summary
RAB13 influences cell surface dynamics by controlling the trafficking of proteins to the plasma membrane. It participates in forming complexes with other RAB proteins ensuring precise membrane delivery and recycling. As a result RAB13 assists in cell junction maintenance and tight junction stability enabling proper cell polarization and epithelial barrier function. This function is essential for processes like cell migration and tissue integrity.
- Pathways
The role of RAB13 in intracellular trafficking links it to essential biological pathways such as the actin cytoskeleton regulation and the signaling of the epithelial-to-mesenchymal transition (EMT). In the actin regulation pathway RAB13 closely interacts with proteins like RHOA and CDC42 which assist in reorganizing the cytoskeleton essential for cell movement. For the EMT process RAB13 plays a part in modulating the cellular adherence and motility pathways which are critical during development and repair mechanisms.
- Associated diseases and disorders
The dysregulation of RAB13 expressions has connections to cancer progression and neurological disorders. Studies show that altered RAB13 activity can contribute to invasive cancer phenotypes such as in breast cancer by facilitating abnormal epithelial cell movement. Additionally improper RAB13 function links to neurological conditions where axonal transport is disrupted highlighting a connection with proteins like TAU which similarly plays a role in the maintenance of the neuronal cytoskeleton.
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1 product image
SDS-PAGE - Recombinant Human RAB13 protein (ab109958)
15% SDS-PAGE analysis of 3 μg ab109958.
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