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AB117218

Recombinant Human RAB23 protein

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Recombinant Human RAB23 protein is a Human Full Length protein, in the 1 to 234 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

HSPC137, RAB23, Ras-related protein Rab-23

1 Images
SDS-PAGE - Recombinant Human RAB23 protein (AB117218)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RAB23 protein (AB117218)

15% SDS-PAGE analysis of ab117218 (3μg)

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9ULC3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMLEEDMEVAIKMVVVGNGAVGKSSMIQRYCKGIFTKDYKKTIGVDFLERQIQVNDEDVRLMLWDTAGQEEFDAITKAYYRGAQACVLVFSTTDRESFEAVSSWREKVVAEVGDIPTVLVQNKIDLLDDSCIKNEEAEALAKRLKLRFYRTSVKEDLNVNEVFKYLAEKYLQKLKQQIAEDPELTHSSSNKIGVFNTSGGSHSGQNSGTLNGGDVINLRPNKQRTKKNRNPFSSC","proteinLength":"Full Length","predictedMolecularWeight":"28.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":234,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9ULC3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB23 also known as Ras-related protein Rab-23 is a small GTPase of the RAB family. It has a molecular weight of approximately 25 kDa. This protein is expressed in various tissues with significant expression in the brain skin and kidney. RAB23 acts as a molecular switch by cycling between inactive GDP-bound and active GTP-bound states. It regulates intracellular vesicle trafficking influencing the movement and fusion of vesicles within the cell.
Biological function summary

RAB23 plays an important role in modulating various cellular processes such as intracellular transport and signal transduction. It interacts with effector proteins through its active GTP-bound form which allows it to facilitate certain cellular functions. RAB23 is known to associate with specific protein complexes contributing to pathways that affect cellular movement and organization reflecting its importance in cellular dynamics.

Pathways

RAB23 is a part of the Hedgehog signaling pathway involved in embryonic development and cell differentiation. Within this pathway RAB23 regulates the activity of proteins like GLI transcription factors to control gene expression important for developmental processes. Additionally RAB23 interacts with other GTPases linking to the actin cytoskeleton remodeling pathway which impacts cellular structure and polarity.

RAB23 has been linked to certain congenital conditions such as Carpenter syndrome which involves malformations in the skull and limbs. Mutations in the RAB23 gene can disrupt normal Hedgehog signaling leading to developmental defects. Furthermore the protein's association with GLI transcription factors connects it to various developmental disorders marked by alterations in early patterning and organ formation. Such links underline the significance of RAB23 in developmental biology and its potential as a therapeutic target.
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Specifications

Form

Liquid

Additional notes

ab117218 was purified using conventional chromatography.

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General info

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Subcellular localisation

Endosome membrane

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Product protocols

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Together with SUFU, prevents nuclear import of GLI1, and thereby inhibits GLI1 transcription factor activity. Regulates GLI1 in differentiating chondrocytes. Likewise, regulates GLI3 proteolytic processing and modulates GLI2 and GLI3 transcription factor activity. Plays a role in autophagic vacuole assembly, and mediates defense against pathogens, such as S.aureus, by promoting their capture by autophagosomes that then merge with lysosomes.
See full target information RAB23
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