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AB276699

Recombinant Human RAB27B protein (His tag)

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Recombinant Human RAB27B protein (His tag) is a Human Fragment protein, in the 2 to 215 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

View Alternative Names

Ras-related protein Rab-27B, C25KG, RAB27B

1 Images
SDS-PAGE - Recombinant Human RAB27B protein (His tag) (AB276699)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RAB27B protein (His tag) (AB276699)

SDS-PAGE analysis of ab276699

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O00194

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS

storage-buffer

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Complementary Products

Primary Antibodies

AB317264

Anti-RAB27B antibody [EPR28647-45]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB27B antibody [EPR28647-45] (AB317264)

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Proteins & Peptides

AB101108

Recombinant Human TNIP1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human TNIP1 protein (His tag N-Terminus) (AB101108)

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Proteins & Peptides

AB98233

Recombinant Human THTPA protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human THTPA protein (His tag N-Terminus) (AB98233)

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Proteins & Peptides

AB95890

Recombinant Human PC4 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human PC4 protein (His tag N-Terminus) (AB95890)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"TDGDYDYLIKLLALGDSGVGKTTFLYRYTDNKFNPKFITTVGIDFREKRVVYNAQGPNGSSGKAFKVHLQLWDTAGQERFRSLTTAFFRDAMGFLLMFDLTSQQSFLNVRNWMSQLQANAYCENPDIVLIGNKADLPDQREVNERQARELADKYGIPYFETSAATGQNVEKAVETLLDLIMKRMEQCVEKTQIPDTVNGGNSGNLDGEKPPEKK","proteinLength":"Fragment","predictedMolecularWeight":"26.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":215,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O00194","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Lyophilized
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB27B also known as Ras-related protein Rab-27B belongs to the Rab family of small GTPases and has a molecular mass of approximately 25 kDa. RAB27B is involved in intracellular vesicle trafficking processes. It is expressed in various tissues with notable expression in platelets and secretory cells. The protein cycles between GDP-bound inactive and GTP-bound active states regulates cargo delivery and maintains distinct cellular compartments.
Biological function summary

The functional role of RAB27B includes mediating the transport and exocytosis of secretory vesicles. This protein collaborates with effector molecules such as MLPH (melanophilin) as part of a multiprotein complex that facilitates vesicle membrane docking and fusion. RAB27B's activity ensures proper cellular secretion processes impacting hormone release pigment distribution and other essential secretory pathways.

Pathways

RAB27B participates in the exosome secretion and regulated secretory pathways. It closely interacts with other proteins including RAB27A which shares similar trafficking functions. RAB27B plays a role in specialized secretory pathways affecting the maturation and docking of vesicles in cells like melanocytes and cytotoxic T lymphocytes which are essential for efficient immune responses and pigmentation respectively.

RAB27B is relevant in conditions such as cancer and Griscelli syndrome. Its dysregulation can contribute to tumor progression and metastasis due to altered vesicle trafficking and secretion dynamics. In Griscelli syndrome type 2 mutations in the related protein RAB27A although not directly in RAB27B impact melanosome transport leading to pigmentary issues and immune dysfunction. Understanding RAB27B's involvement offers insights into potential therapeutic strategies targeting these pathways.
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General info

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 21775604, PubMed : 30771381). RAB27B regulates homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed : 30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed : 21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Subcellular localisation

Late endosome

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Product protocols

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 21775604, PubMed : 30771381). RAB27B regulates homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion (PubMed : 30771381). Plays a role in NTRK2/TRKB axonal anterograde transport by facilitating the association of NTRK2/TRKB with KLC1 (PubMed : 21775604). May be involved in targeting uroplakins to urothelial apical membranes (By similarity).
See full target information RAB27B
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