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Recombinant Human RAB29 protein is a Human Full Length protein, in the 1 to 203 aa range, expressed in Wheat germ and suitable for ELISA, WB.

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Images

SDS-PAGE - Recombinant Human RAB29 protein (AB160266), expandable thumbnail

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

Biologically active

No

Amino acid sequence

M G S R D H L F K V L V V G D A A V G K T S L V Q R Y S Q D S F S K H Y K S T V G V D F A L K V L Q W S D Y E I V R L Q L W D I A G Q E R F T S M T R L Y Y R D A S A C V I M F D V T N A T T F S N S Q R W K Q D L D S K L T L P N G E P V P C L L L A N K C D L S P W A V S R D Q I D R F S K E N G F T G W T E T S V K E N K N I N E A M R V L I E K M M R N S T E D I M S L S T Q G D Y I N L Q T K S S S W S C C

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

The small GTPases Rab are key regulators in vesicle trafficking (PubMed:24788816). Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed:24788816). Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity (PubMed:29212815, PubMed:38127736). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736). Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection (PubMed:22042847).

Alternative names

Recommended products

Recombinant Human RAB29 protein is a Human Full Length protein, in the 1 to 203 aa range, expressed in Wheat germ and suitable for ELISA, WB.

Key facts

Expression system

Wheat germ

Applications

ELISA, WB

Accession
O14966-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M G S R D H L F K V L V V G D A A V G K T S L V Q R Y S Q D S F S K H Y K S T V G V D F A L K V L Q W S D Y E I V R L Q L W D I A G Q E R F T S M T R L Y Y R D A S A C V I M F D V T N A T T F S N S Q R W K Q D L D S K L T L P N G E P V P C L L L A N K C D L S P W A V S R D Q I D R F S K E N G F T G W T E T S V K E N K N I N E A M R V L I E K M M R N S T E D I M S L S T Q G D Y I N L Q T K S S S W S C C

Accession

O14966

Protein length

Full Length

Amino acids

1 to 203

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

General info

Function

The small GTPases Rab are key regulators in vesicle trafficking (PubMed:24788816). Essential for maintaining the integrity of the endosome-trans-Golgi network structure (By similarity). Together with LRRK2, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner (PubMed:24788816). Recruits LRRK2 to the Golgi complex and stimulates LRRK2 kinase activity (PubMed:29212815, PubMed:38127736). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736). Regulates neuronal process morphology in the intact central nervous system (CNS) (By similarity). May play a role in the formation of typhoid toxin transport intermediates during Salmonella enterica serovar Typhi (S.Typhi) epithelial cell infection (PubMed:22042847).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Post-translational modifications

In case of Salmonella enterica serovar Typhimurium (S.Typhimurium) infection, is proteolytically cleaved between Gly-41 and Val-42 by the GtgE viral protease encoded on the Gifsy-2 lysogen bacteriophage, which therefore prevents the recruitment of RAB29 to S.Typhimurium-containing vacuoles. In contrast, no proteolytically cleavage is detected in S.Typhi-infected cells (PubMed:22042847).

Subcellular localisation

Vacuole, Cytoskeleton

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

U+00A0;

U+00A0;

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RAB29 also known as Rab7L1 is a member of the RAB family of small GTPases. This protein is involved in vesicle trafficking processes within cells. RAB29 has an approximate mass of 23 kDa. It is expressed in various tissues but shows higher expression levels in brain and testis. In cellular functions it acts mainly within the endosomal-lysosomal system to regulate membrane trafficking events.

Biological function summary

RAB29 plays an important role in ensuring the proper transport and sorting of proteins within cells. It associates with certain effector proteins to form complexes which facilitate movement between endosomes and other intracellular compartments. It coordinates with other RAB proteins to maintain cellular homeostasis and ensure effective intracellular transport. This coordination is significant for neural cells suggesting a specific importance in neurological environments.

Pathways

RAB29 engages in intracellular trafficking pathways that include the endocytic pathway and Golgi-lysosomal trafficking. In these pathways it cooperates closely with proteins like LRRK2 to regulate autophagy and the maintenance of the endosomal membrane system. Through these pathways it maintains cellular organelle distribution and affects lysosome functionality impacting overall cell health and metabolism.

Associated diseases and disorders

RAB29 exhibits connections to neurodegenerative disorders such as Parkinson’s disease. Mutations or dysfunctions in RAB29 along with its interaction partner LRRK2 have been linked to the pathogenesis of this disorder implicating it in the degeneration of dopaminergic neurons. Additionally aberrant RAB29 activity has been associated with complications in lysosomal storage diseases suggesting its broader implications in cellular waste handling and turnover.

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1 product image

  • SDS-PAGE - Recombinant Human RAB29 protein (ab160266), expandable thumbnail

    SDS-PAGE - Recombinant Human RAB29 protein (ab160266)

    ab160266 on a 12.5% SDS-PAGE stained with Coomassie Blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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