Recombinant Human RAB31 protein is a Human Full Length protein, in the 1 to 195 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H L H K F L I W D T A G Q E R F H S L A P M Y Y R G S A A A V I V Y D I T K Q D S F Y T L K K W V K E L K E H G P E N I V M A I A G N K C D L S D I R E V P L K D A K E Y A E S I G A I V V E T S A K N A I N I E E L F Q G I S R Q I P P L D P H E N G N N G T I K V E K P T M Q A S R R C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
Alternative names
RAB22B, RAB31, Ras-related protein Rab-31, Ras-related protein Rab-22B
Recommended products
Recombinant Human RAB31 protein is a Human Full Length protein, in the 1 to 195 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q13636-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H L H K F L I W D T A G Q E R F H S L A P M Y Y R G S A A A V I V Y D I T K Q D S F Y T L K K W V K E L K E H G P E N I V M A I A G N K C D L S D I R E V P L K D A K E Y A E S I G A I V V E T S A K N A I N I E E L F Q G I S R Q I P P L D P H E N G N N G T I K V E K P T M Q A S R R C
- Accession
- Q13636
- Protein length
- Full Length
- Predicted molecular weight
- 25.9 kDa
- Amino acids
- 1 to 195
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab124565 was purified by conventional chromatography techniques.
General info
- Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
- Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
- Subcellular localisation
- Early endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB31 also known as RAB22B belongs to the RAB family which plays an important role in vesicular trafficking. It is a small GTPase with a mass of approximately 23 kDa. RAB31 is mainly expressed in epithelial tissues and certain cancer cell lines. It regulates vesicle formation movement and fusion by cycling between an active GTP-bound state and an inactive GDP-bound state. This cycling is important for its function in the intracellular transport system.
- Biological function summary
RAB31 influences early endosome dynamics and directly impacts the sorting and recycling of receptors. It associates with effector proteins to form protein complexes that facilitate endocytosis and exocytosis processes. It also interacts with other Rab proteins to ensure proper membrane specificity and temporal regulation within cellular compartments. This behavior highlights its role in maintaining cellular homeostasis and responsiveness to environmental changes.
- Pathways
Studies have shown that RAB31 contributes significantly to the endocytic pathway and the insulin signaling pathway. It has functional interactions with proteins like RAB5 and RAB11 both of which are key regulators of endosomal trafficking. RAB31 ensures a proper transport cycle that influences receptor recycling and signaling impacting how cells respond to growth factors and hormones.
- Associated diseases and disorders
Research implicates RAB31 in the progression of certain cancers such as breast cancer due to its established role in vesicle trafficking and receptor recycling which can affect cell growth and division. Its altered expression levels may intersect with proteins such as EGFR leading to abnormal signaling cascades. Furthermore RAB31 has associations with metabolic disorders as disruptions in insulin signaling pathways may contribute to diabetes showcasing its diverse influence on human health.
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1 product image
SDS-PAGE - Recombinant Human RAB31 protein (ab124565)
ab124565 (3µg) visualised by SDS-PAGE (15%).
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