Recombinant Human RAB32 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M A G G G A G D P G L G A A A A P A P E T R E H L F K V L V I G E L G V G K T S I I K R Y V H Q L F S Q H Y R A T I G V D F A L K V L N W D S R T L V R L Q L W D I A G Q E R F G N M T R V Y Y K E A V G A F V V F D I S R S S T F E A V L K W K S D L D S K V H L P N G S P I P A V L L A N K C D Q N K D S S Q S P S Q V D Q F C K E H G F A G W F E T S A K D N I N I E E A A R F L V E K I L V N H Q S F P N E E N D V D K I K L D Q E T L R A E N K S Q C C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed:12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed:21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed:23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736).
Alternative names
Ras-related protein Rab-32, RAB32
Recommended products
Recombinant Human RAB32 protein is a Human Full Length protein, in the 1 to 225 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q13637-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.06% EDTA
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M A G G G A G D P G L G A A A A P A P E T R E H L F K V L V I G E L G V G K T S I I K R Y V H Q L F S Q H Y R A T I G V D F A L K V L N W D S R T L V R L Q L W D I A G Q E R F G N M T R V Y Y K E A V G A F V V F D I S R S S T F E A V L K W K S D L D S K V H L P N G S P I P A V L L A N K C D Q N K D S S Q S P S Q V D Q F C K E H G F A G W F E T S A K D N I N I E E A A R F L V E K I L V N H Q S F P N E E N D V D K I K L D Q E T L R A E N K S Q C C
- Accession
- Q13637
- Protein length
- Full Length
- Predicted molecular weight
- 27.6 kDa
- Amino acids
- 1 to 225
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab113131 was purified using conventional chromatography techniques.
General info
- Function
Acts as an A-kinase anchoring protein by binding to the type II regulatory subunit of protein kinase A and anchoring it to the mitochondrion. Also involved in synchronization of mitochondrial fission (PubMed:12186851). Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis (PubMed:21255211). Plays an important role in the control of melanin production and melanosome biogenesis (PubMed:23084991). In concert with RAB38, regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (By similarity). Stimulates phosphorylation of RAB10 'Thr-73' by LRRK2 (PubMed:38127736).
- Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
- Subcellular localisation
- Mitochondrion, Mitochondrion outer membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB32 is a member of the RAB protein family involved in intracellular membrane trafficking. It is sometimes called Ras-related protein RAB32. The molecular mass of RAB32 is about 26 kilodaltons. It locates itself primarily in the cytoplasm and various organelles such as the mitochondria and endosomes. RAB32 has a role in vesicle trafficking acting as a molecular switch that toggles between active GTP-bound and inactive GDP-bound states managing the transport and fusion of cellular vesicles.
- Biological function summary
The normal function of RAB32 influences several cellular processes including pigmentation and energy metabolism. It does not usually act alone but is part of larger protein complexes that regulate intracellular processes. RAB32 contributes to mitochondrial fission and fusion as well as melanosome biogenesis. Its interaction with other proteins helps establish a cellular environment compatible with various organelle functions.
- Pathways
Its role is significant in key cellular pathways like the cAMP signaling pathway and the autophagy-lysosome pathway. RAB32 interacts with proteins such as PKA and ATG16L1 to modulate these pathways. In the cAMP pathway RAB32 affects protein kinase A activity modifying cellular responses to external signals. In the autophagy process it assists in lysosome function contributing to cellular cleanup and energy homeostasis.
- Associated diseases and disorders
The dysfunction of RAB32 has connections with some types of cancer and neurodegenerative diseases. Its overexpression or aberrant regulation is often seen in melanoma development where it can disrupt normal pigmentation processes. Additionally in neurodegenerative conditions like Alzheimer's disease RAB32 dysregulation might influence mitochondrial dynamics as it interacts with proteins like DRP1. Understanding these connections provides potential targets for therapeutic interventions in these diseases.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human RAB32 protein (ab113131)
15% SDS-PAGE analysis of 3 μg ab113131.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com