Recombinant Human RAB33B protein is a Human Full Length protein, in the 1 to 229 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M A E E M E S S L E A S F S S S G A V S G A S G F L P P A R S R I F K I I V I G D S N V G K T C L T Y R F C A G R F P D R T E A T I G V D F R E R A V E I D G E R I K I Q L W D T A G Q E R F R K S M V Q H Y Y R N V H A V V F V Y D M T N M A S F H S L P S W I E E C K Q H L L A N D I P R I L V G N K C D L R S A I Q V P T D L A Q K F A D T H S M P L F E T S A K N P N D N D H V E A I F M T L A H K L K S H K P L M L S Q P P D N G I I L K P E P K P A M T C W C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking. It is involved in autophagy, acting as a modulator of autophagosome formation.
Alternative names
Ras-related protein Rab-33B, RAB33B
Recommended products
Recombinant Human RAB33B protein is a Human Full Length protein, in the 1 to 229 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9H082-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A E E M E S S L E A S F S S S G A V S G A S G F L P P A R S R I F K I I V I G D S N V G K T C L T Y R F C A G R F P D R T E A T I G V D F R E R A V E I D G E R I K I Q L W D T A G Q E R F R K S M V Q H Y Y R N V H A V V F V Y D M T N M A S F H S L P S W I E E C K Q H L L A N D I P R I L V G N K C D L R S A I Q V P T D L A Q K F A D T H S M P L F E T S A K N P N D N D H V E A I F M T L A H K L K S H K P L M L S Q P P D N G I I L K P E P K P A M T C W C
- Accession
- Q9H082
- Protein length
- Full Length
- Amino acids
- 1 to 229
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Protein transport. Acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking. It is involved in autophagy, acting as a modulator of autophagosome formation.
- Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB33B also known as Ras-related protein Rab-33B is a small GTPase involved in intracellular vesicle trafficking. It has a molecular mass of about 25 kDa. RAB33B localizes mainly to the Golgi apparatus. It functions in regulating vesicle movement within the Golgi and between the Golgi and other organelles. This protein is widely expressed in various tissues suggesting its broad role in cellular processes.
- Biological function summary
RAB33B interacts actively with other proteins like Golgins and SNAREs to mediate vesicle budding transport and fusion. It is part of a complex that influences Golgi structure and function playing a role in maintaining cellular homeostasis. Through its GTPase activity RAB33B helps manage the directionality and specificity of vesicle trafficking. This positions it as an important player in cellular logistics ensuring protein and lipid cargoes reach their destination effectively.
- Pathways
The protein's function links it to the post-Golgi vesicular transport pathway. This pathway is critical for transporting proteins from the Golgi to their final destinations including the cell membrane. RAB33B also integrates with the GTPase cycle pathway working alongside other proteins like RAB1 and RAB6 which facilitate different stages of vesicle transport. These interactions ensure the smooth progression of Golgi-related pathways affecting various cellular functions.
- Associated diseases and disorders
RAB33B has been associated with Smith-McCort dysplasia a bone development disorder. Mutations affecting RAB33B disrupt normal Golgi function leading to defects in collagen secretion which impacts skeletal formation. Additionally its interactions with proteins like RAB1 in the context of disease have drawn attention to its potential role in viral infections as RAB proteins often play roles in the life cycle of certain viruses. Understanding these connections can provide insights into therapeutic targets and strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human RAB33B protein (ab164395)
ab164395 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com