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AB164395

Recombinant Human RAB33B protein (GST tag N-Terminus)

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Recombinant Human RAB33B protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 229 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

Ras-related protein Rab-33B, RAB33B

1 Images
SDS-PAGE - Recombinant Human RAB33B protein (GST tag N-Terminus) (AB164395)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RAB33B protein (GST tag N-Terminus) (AB164395)

ab164395 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

Q9H082

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Primary Antibodies

AB155679

Anti-RAB33B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB33B antibody (AB155679)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MAEEMESSLEASFSSSGAVSGASGFLPPARSRIFKIIVIGDSNVGKTCLTYRFCAGRFPDRTEATIGVDFRERAVEIDGERIKIQLWDTAGQERFRKSMVQHYYRNVHAVVFVYDMTNMASFHSLPSWIEECKQHLLANDIPRILVGNKCDLRSAIQVPTDLAQKFADTHSMPLFETSAKNPNDNDHVEAIFMTLAHKLKSHKPLMLSQPPDNGIILKPEPKPAMTCWC","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":229,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q9H082","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB33B also known as Ras-related protein Rab-33B is a small GTPase involved in intracellular vesicle trafficking. It has a molecular mass of about 25 kDa. RAB33B localizes mainly to the Golgi apparatus. It functions in regulating vesicle movement within the Golgi and between the Golgi and other organelles. This protein is widely expressed in various tissues suggesting its broad role in cellular processes.
Biological function summary

RAB33B interacts actively with other proteins like Golgins and SNAREs to mediate vesicle budding transport and fusion. It is part of a complex that influences Golgi structure and function playing a role in maintaining cellular homeostasis. Through its GTPase activity RAB33B helps manage the directionality and specificity of vesicle trafficking. This positions it as an important player in cellular logistics ensuring protein and lipid cargoes reach their destination effectively.

Pathways

The protein's function links it to the post-Golgi vesicular transport pathway. This pathway is critical for transporting proteins from the Golgi to their final destinations including the cell membrane. RAB33B also integrates with the GTPase cycle pathway working alongside other proteins like RAB1 and RAB6 which facilitate different stages of vesicle transport. These interactions ensure the smooth progression of Golgi-related pathways affecting various cellular functions.

RAB33B has been associated with Smith-McCort dysplasia a bone development disorder. Mutations affecting RAB33B disrupt normal Golgi function leading to defects in collagen secretion which impacts skeletal formation. Additionally its interactions with proteins like RAB1 in the context of disease have drawn attention to its potential role in viral infections as RAB proteins often play roles in the life cycle of certain viruses. Understanding these connections can provide insights into therapeutic targets and strategies.
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Specifications

Form

Liquid

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General info

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 20163571, PubMed : 21808068). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 18448665, PubMed : 20163571, PubMed : 21808068). RAB33B acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (PubMed : 20163571). Participates in autophagosome formation by recruiting the ATG12-ATG5-ATG16L1 complex to phagophores, probably in a nucleotide-independent manner (PubMed : 18448665, PubMed : 32960676).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Post-translational modifications

Prenylated.

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Product protocols

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes (PubMed : 20163571, PubMed : 21808068). Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 18448665, PubMed : 20163571, PubMed : 21808068). RAB33B acts, in coordination with RAB6A, to regulate intra-Golgi retrograde trafficking (PubMed : 20163571). Participates in autophagosome formation by recruiting the ATG12-ATG5-ATG16L1 complex to phagophores, probably in a nucleotide-independent manner (PubMed : 18448665, PubMed : 32960676).
See full target information Ras-related protein Rab-33B
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