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AB109943

Recombinant Human Rab3D protein

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Recombinant Human Rab3D protein is a Human Full Length protein, in the 1 to 219 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

GOV, RAB16, RAB3D, Ras-related protein Rab-3D

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SDS-PAGE - Recombinant Human Rab3D protein (AB109943)
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SDS-PAGE - Recombinant Human Rab3D protein (AB109943)

15% SDS-PAGE analysis of 3μg ab109943.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O95716

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 30% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.116% Sodium chloride, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC","proteinLength":"Full Length","predictedMolecularWeight":"26.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":219,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95716","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab3D also known as Ras-related protein Rab-3D belongs to the Rab family of small GTPases. It has a mass of approximately 25 kDa. Rab3D functions as a molecular switch that cycles between an inactive GDP-bound state and an active GTP-bound state regulating membrane trafficking processes. This protein is highly expressed in secretory tissues such as exocrine pancreas salivary glands and osteoclasts where it plays a role in the secretory pathway.
Biological function summary

Rab3D plays an essential role in vesicle exocytosis modulating the secretion of enzymes hormones and other secretory products. Much of Rab3D's function depends on its interaction with other vesicle-associated proteins including Rab3-interacting molecules (RIMs) which are part of the secretory complex. Rab3D ensures proper docking and fusion of secretory vesicles with the plasma membrane.

Pathways

The Rab3D protein influences the exocytosis and endocytic pathways. It closely interacts with other members of the Rab family especially Rab27 and Rab8 which orchestrate various sub-tasks in vesicle trafficking. These pathways are vital for vesicle-mediated transport and require the regulated activity of Rab3D for proper cellular communication and secretion.

Rab3D links to bone resorption disorders specifically related to osteoclast function and bone density diseases like osteoporosis. Dysregulation in Rab3D activity can influence osteoclast function where Rab3D works alongside proteins such as Rab27a contributing to the excessive bone resorption noted in osteoporosis. Additionally altered Rab3D function may have implications in disorders related to exocrine gland secretion impacting conditions like Sjögren's syndrome.
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Specifications

Form

Liquid

Additional notes

ab109943 was purified using conventional chromatography techniques.

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General info

Function

Protein transport. Probably involved in regulated exocytosis (By similarity).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Post-translational modifications

Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and CHML.

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Product protocols

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Target data

Protein transport. Probably involved in regulated exocytosis (By similarity).
See full target information RAB3D
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Complementary Products

Primary Antibodies

AB128997

Anti-Rab3D antibody [EPR8106]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (AB128997)

  • 5
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Primary Antibodies

AB320737

Anti-GOLM1/GOLPH2 antibody [EPR29022-61]

BOND RX™ Validated|20ul selling size|KO Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLM1/GOLPH2 antibody [EPR29022-61] (AB320737)

  • 0
  • 0
Primary Antibodies

AB232895

Anti-CPLX2 antibody

Western blot - Anti-CPLX2 antibody (AB232895)

  • 0
  • 0
Primary Antibodies

AB185211

Anti-RHOD antibody [EPR7027] - N-terminal

RabMAb|Recombinant

Western blot - Anti-RHOD antibody [EPR7027] - N-terminal (AB185211)

  • 0
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