Recombinant Human Rab5 protein (His tag) is a Human Full Length protein, in the 1 to 215 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M A S R G A T R P N G P N T G N K I C Q F K L V L L G E S A V G K S S L V L R F V K G Q F H E F Q E S T I G A A F L T Q T V C L D D T T V K F E I W D T A G Q E R Y H S L A P M Y Y R G A Q A A I V V Y D I T N E E S F A R A K N W V K E L Q R Q A S P N I V I A L S G N K A D L A N K R A V D F Q E A Q S Y A D D N S L L F M E T S A K T S M N V N E I F M A I A K K L P K N E P Q N P G A N S A R G R G V D L T E P T Q P T R N Q C C S N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
Alternative names
RAB5, RAB5A, Ras-related protein Rab-5A
Recommended products
Recombinant Human Rab5 protein (His tag) is a Human Full Length protein, in the 1 to 215 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- P20339-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
Constituents: 10% Glycerol (glycerin, glycerine), 2.61% Sodium chloride, 0.32% Tris HCl, 0.08% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M A S R G A T R P N G P N T G N K I C Q F K L V L L G E S A V G K S S L V L R F V K G Q F H E F Q E S T I G A A F L T Q T V C L D D T T V K F E I W D T A G Q E R Y H S L A P M Y Y R G A Q A A I V V Y D I T N E E S F A R A K N W V K E L Q R Q A S P N I V I A L S G N K A D L A N K R A V D F Q E A Q S Y A D D N S L L F M E T S A K T S M N V N E I F M A I A K K L P K N E P Q N P G A N S A R G R G V D L T E P T Q P T R N Q C C S N
- Accession
- P20339
- Protein length
Full Length
- Predicted molecular weight
26 kDa
- Amino acids
1 to 215
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Affinity Purified
General info
- Function
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
- Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
- Post-translational modifications
Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.
- Subcellular localisation
Early endosome membrane, Endosome membrane
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rab5 also known as Ras-related protein Rab-5A is a small GTPase of approximately 24 kDa. It is part of the Rab family of proteins and functions as an endosomal marker in cells. Rab5 is ubiquitously expressed but shows enriched expression in tissues with a high rate of endocytosis like the brain and liver. This protein plays a significant role in early endosome fusion and trafficking acting as an essential regulator of vesicular transport processes within the cell.
- Biological function summary
Rab5 regulates the early endocytic pathway by mediating vesicle docking and fusion processes. It is not a part of a large complex but interacts with a range of effector proteins that facilitate endosome maturation. Rab5's role as an endosomal marker makes it important for membrane trafficking receptor recycling and signal transduction. Researchers often identify Rab5 in cellular studies using endosome staining techniques or specific antibodies like Drosophila antibodies to visualize its function and distribution.
- Pathways
The endocytic network heavily involves Rab5 particularly influencing the endocytosis and endosomal signaling pathways. It frequently interacts with proteins such as EEA1 (Early Endosome Antigen 1) and Rabaptin-5 which are critical for endosomal membrane fusion. This mechanistic involvement ensures the correct transfer and processing of endocytic vesicles facilitating the continual cycle of membrane and receptor turnover in cells.
- Associated diseases and disorders
Rab5 dysfunction associates with neurological conditions such as Alzheimer's disease and cancer. Alterations in Rab5 levels or function can disrupt normal endosomal trafficking leading to amyloid beta peptide accumulation in Alzheimer's. Additionally Rab5's role in cancer relates to its regulation of growth factor receptors where its interaction with proteins like the PE marker influences tumor progression. Understanding Rab5 pathways and interactions paves the way for developing targeted therapeutics in these disorders.
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SDS-PAGE - Recombinant Human Rab5 protein (His tag) (ab245929)
SDS-PAGE analysis of Recombinant Human Rab5 protein (His tag) (ab245929)
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