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Recombinant Human Rab5A protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.

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Images

Western blot - Recombinant Human Rab5A protein (AB62956), expandable thumbnail
  • SDS-PAGE - Recombinant Human Rab5A protein (AB62956), expandable thumbnail
  • Western blot - Recombinant Human Rab5A protein (AB62956), expandable thumbnail

Key facts

Purity
>95% SDS-PAGE
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, WB
Biologically active
No

Amino acid sequence

M A S R G A T R P N G P N T G N K I C Q F K L V L L G E S A V G K S S L V L R F V K G Q F H E F Q E S T I G A A F L T Q T V C L D D T T V K F E I W D T A G Q E R Y H S L A P M Y Y R G A Q A A I V V Y D I T N E E S F A R A K N W V K E L Q R Q A S P N I V I A L S G N K A D L A N K R A V D F Q E A Q S Y A D D N S L L F M E T S A K T S M N V N E I F M A I A K K L P K N E P Q N P G A N S A R G R G V D L T E P T Q P T R N Q C C S N

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

ab62956 can be used as a WB positive control in conjunction with Anti-Rab5 antibody - Early Endosome Marker ab18211.

Associated Products

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2 products for Alternative Product

Target data

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Alternative names

RAB5, RAB5A, Ras-related protein Rab-5A

Recommended products

Recombinant Human Rab5A protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.

Key facts

Purity
>95% SDS-PAGE
Expression system
Escherichia coli
Applications
SDS-PAGE, WB
Accession
P20339-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 6
Constituents: PBS, 0.242% Tris

Sequence info

Amino acid sequence

M A S R G A T R P N G P N T G N K I C Q F K L V L L G E S A V G K S S L V L R F V K G Q F H E F Q E S T I G A A F L T Q T V C L D D T T V K F E I W D T A G Q E R Y H S L A P M Y Y R G A Q A A I V V Y D I T N E E S F A R A K N W V K E L Q R Q A S P N I V I A L S G N K A D L A N K R A V D F Q E A Q S Y A D D N S L L F M E T S A K T S M N V N E I F M A I A K K L P K N E P Q N P G A N S A R G R G V D L T E P T Q P T R N Q C C S N
Accession
P20339
Protein length
Full Length
Nature
Recombinant

Specifications

Form
Liquid
Additional notes

purified by using conventional chromatography techniques

General info

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:15378032, PubMed:16410077). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Post-translational modifications

Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.

Subcellular localisation
Early endosome membrane, Endosome membrane

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rab5A also known as RAB5A or Ras-related protein Rab-5A is a small GTPase with a molecular weight of approximately 24 kDa. Rab5A plays a role in the regulation of endocytic trafficking. It switches between an active GTP-bound state and an inactive GDP-bound state which facilitates its function in vesicle fusion and transport. Rab5A is highly expressed in various tissues with significant expression in the brain liver and kidney indicating its important regulatory role in these organs.

Biological function summary

Rab5A influences the coordination of membrane trafficking and cytoskeletal dynamics. It associates with several protein complexes involved in endocytosis. Rab5A recruits effectors that facilitate the early stages of endosome formation and maturation. It regulates the homotypic fusion and motility of early endosomes by activating its downstream effectors such as EEA1 and Rabenosyn-5 which coordinate vesicle tethering and fusion.

Pathways

Rab5A is intricately involved in the endocytic pathway and the PI3K-Akt signaling pathway. This protein modulates the formation and maturation of endosomes playing a part in the cellular sorting and internalization of receptors. Rab5A interacts with proteins like early endosome antigen 1 (EEA1) in the endocytic pathway and also links to phosphatidylinositol-3-phosphate (PI3P) production which is critical for signaling associated with cellular growth and survival.

Associated diseases and disorders

Rab5A is associated with neurodegenerative diseases such as Alzheimer's disease and cancer progression. Aberrant Rab5A activity leads to disrupted endocytic trafficking which results in the malfunction of cellular processes that contribute to Alzheimer's disease pathology. In cancer Rab5A interaction with proteins like epidermal growth factor receptor (EGFR) modulates signal transduction pathways that facilitate oncogenic transformation and tumor progression. Hence Rab5A represents a potential therapeutic target in these conditions.

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