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AB62956

Recombinant Human Rab5A protein

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Recombinant Human Rab5A protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

RAB5, RAB5A, Ras-related protein Rab-5A

3 Images
Western blot - Recombinant Human Rab5A protein (AB62956)
  • WB

Lab

Western blot - Recombinant Human Rab5A protein (AB62956)

All lanes:

Western blot - Anti-Rab5 antibody [EPR17321] - Early Endosome Marker (<a href='/en-us/products/primary-antibodies/rab5-antibody-epr17321-early-endosome-marker-ab199530'>ab199530</a>) at 1/1000 dilution

Lane 1:

Western blot - Recombinant Human Rab5A protein (ab62956)

Lane 2:

Western blot - Recombinant Human Rab5b protein (<a href='/en-us/products/proteins-peptides/recombinant-human-rab5b-protein-ab104545'>ab104545</a>)

Lane 3:

Western blot - Recombinant Human RAB5C/RABL protein (<a href='/en-us/products/proteins-peptides/recombinant-human-rab5c-rabl-protein-ab156358'>ab156358</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26 kDa

false

Western blot - Recombinant Human Rab5A protein (AB62956)
  • WB

Unknown

Western blot - Recombinant Human Rab5A protein (AB62956)

All lanes:

Western blot - Anti-Rab5 antibody - Early Endosome Marker (<a href='/en-us/products/primary-antibodies/rab5-antibody-early-endosome-marker-ab18211'>ab18211</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Rab5A protein (ab62956) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 24 kDa

true

Exposure time: 150s

SDS-PAGE - Recombinant Human Rab5A protein (AB62956)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Rab5A protein (AB62956)

SDS Page analysis of ab62956 (3 ug)

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P20339

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 6 Constituents: PBS, 0.242% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab62956 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/rab5-antibody-early-endosome-marker-ab18211'>ab18211</a>.</p>" } } }
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Sequence info

[{"sequence":"MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P20339","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rab5A also known as RAB5A or Ras-related protein Rab-5A is a small GTPase with a molecular weight of approximately 24 kDa. Rab5A plays a role in the regulation of endocytic trafficking. It switches between an active GTP-bound state and an inactive GDP-bound state which facilitates its function in vesicle fusion and transport. Rab5A is highly expressed in various tissues with significant expression in the brain liver and kidney indicating its important regulatory role in these organs.
Biological function summary

Rab5A influences the coordination of membrane trafficking and cytoskeletal dynamics. It associates with several protein complexes involved in endocytosis. Rab5A recruits effectors that facilitate the early stages of endosome formation and maturation. It regulates the homotypic fusion and motility of early endosomes by activating its downstream effectors such as EEA1 and Rabenosyn-5 which coordinate vesicle tethering and fusion.

Pathways

Rab5A is intricately involved in the endocytic pathway and the PI3K-Akt signaling pathway. This protein modulates the formation and maturation of endosomes playing a part in the cellular sorting and internalization of receptors. Rab5A interacts with proteins like early endosome antigen 1 (EEA1) in the endocytic pathway and also links to phosphatidylinositol-3-phosphate (PI3P) production which is critical for signaling associated with cellular growth and survival.

Rab5A is associated with neurodegenerative diseases such as Alzheimer's disease and cancer progression. Aberrant Rab5A activity leads to disrupted endocytic trafficking which results in the malfunction of cellular processes that contribute to Alzheimer's disease pathology. In cancer Rab5A interaction with proteins like epidermal growth factor receptor (EGFR) modulates signal transduction pathways that facilitate oncogenic transformation and tumor progression. Hence Rab5A represents a potential therapeutic target in these conditions.
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Specifications

Form

Liquid

Additional notes

purified by using conventional chromatography techniques

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General info

Function

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Post-translational modifications

Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including RAB GDP dissociation inhibitors GDI1 and GDI2.. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3.

Subcellular localisation

Early endosome membrane

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Product protocols

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Target data

Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed : 10818110, PubMed : 14617813, PubMed : 15378032, PubMed : 16410077). Contributes to the regulation of filopodia extension (PubMed : 14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed : 22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).
See full target information RAB5A
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Complementary Products

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Anti-Rab5 antibody [EPR21801] - Early Endosome Marker

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Cell Lines & Lysates

AB258615

Human RAB1A knockout HEK-293T cell lysate

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  • 0
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Primary Antibodies

AB237337

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547] (AB237337)

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