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AB104035

Recombinant Human RAB6B protein

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Recombinant Human RAB6B protein is a Human Full Length protein, in the 1 to 208 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Ras-related protein Rab-6B, RAB6B

1 Images
SDS-PAGE - Recombinant Human RAB6B protein (AB104035)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RAB6B protein (AB104035)

15% SDS-PAGE analysis of ab104035 (3 μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9NRW1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLADKRQITIEEGEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQEKSKEGMIDIKLDKPQEPPASEGGCSC","proteinLength":"Full Length","predictedMolecularWeight":"25.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":208,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9NRW1","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAB6B also known as ras-related protein Rab-6B is a small GTPase with a molecular weight of approximately 24 kDa. It plays a mechanical role in intracellular trafficking processes specifically in the regulation of vesicular transport and Golgi apparatus function. RAB6B is widely expressed in human tissues with heightened levels observed in brain tissue particularly in cerebral cortex neurons and the central nervous system. This makes it important for understanding neurological processes and maintaining cellular homeostasis in such tissues.
Biological function summary

RAB6B acts in coordination with components of the vesicle transport machinery. It is not part of a large protein complex but interacts with several regulatory proteins to ensure accurate vesicle movement and docking within cells. This protein mainly influences retrograde transport from the Golgi back to the endoplasmic reticulum. This activity supports the recycling of proteins and lipids aiding in the proper maintenance of the Golgi structure and overall cellular function.

Pathways

RAB6B modulates significant pathways related to membrane trafficking. It has pronounced involvement in the retrograde vesicle transport pathway impacting the transport of cargos in neurons. RAB6B's interaction with other RAB proteins especially with members such as RAB3 and RAB8 influences synaptic vesicle exocytosis and endosomal sorting. This highlights its role in neurotransmitter release and synaptic plasticity which are important for neuronal communication.

Abnormalities in RAB6B expression and function associate with neurological disorders particularly Alzheimer's disease and Autism Spectrum Disorder. In Alzheimer's RAB6B's interaction with amyloid precursor protein (APP) influences amyloid-beta peptide generation an important event in disease pathology. In autism altered RAB6B activities potentially affect synaptic vesicle trafficking leading to impaired neural connectivity and function. Understanding these connections can provide deeper insights into the mechanisms behind these conditions.
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Specifications

Form

Liquid

Additional notes

Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology (Probable). Recruits VPS13B to the Golgi membrane (PubMed : 25492866). Regulates the compacted morphology of the Golgi (PubMed : 26209634). Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells (PubMed : 17707369). Plays a role in neuron projection development (PubMed : 25492866).

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

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Product protocols

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Target data

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology (Probable). Recruits VPS13B to the Golgi membrane (PubMed : 25492866). Regulates the compacted morphology of the Golgi (PubMed : 26209634). Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells (PubMed : 17707369). Plays a role in neuron projection development (PubMed : 25492866).
See full target information Ras-related protein Rab-6B
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Secondary Antibodies

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Primary Antibodies

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Secondary Antibodies

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