Recombinant Human RAB6B protein is a Human Full Length protein, in the 1 to 208 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S A G G D F G N P L R K F K L V F L G E Q S V G K T S L I T R F M Y D S F D N T Y Q A T I G I D F L S K T M Y L E D R T V R L Q L W D T A G Q E R F R S L I P S Y I R D S T V A V V V Y D I T N L N S F Q Q T S K W I D D V R T E R G S D V I I M L V G N K T D L A D K R Q I T I E E G E Q R A K E L S V M F I E T S A K T G Y N V K Q L F R R V A S A L P G M E N V Q E K S K E G M I D I K L D K P Q E P P A S E G G C S C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology (Probable). Recruits VPS13B to the Golgi membrane (PubMed:25492866). Regulates the compacted morphology of the Golgi (PubMed:26209634). Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells (PubMed:17707369). Plays a role in neuron projection development (PubMed:25492866).
Alternative names
Ras-related protein Rab-6B, RAB6B
Recommended products
Recombinant Human RAB6B protein is a Human Full Length protein, in the 1 to 208 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NRW1-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S A G G D F G N P L R K F K L V F L G E Q S V G K T S L I T R F M Y D S F D N T Y Q A T I G I D F L S K T M Y L E D R T V R L Q L W D T A G Q E R F R S L I P S Y I R D S T V A V V V Y D I T N L N S F Q Q T S K W I D D V R T E R G S D V I I M L V G N K T D L A D K R Q I T I E E G E Q R A K E L S V M F I E T S A K T G Y N V K Q L F R R V A S A L P G M E N V Q E K S K E G M I D I K L D K P Q E P P A S E G G C S C
- Accession
- Q9NRW1
- Protein length
- Full Length
- Predicted molecular weight
- 25.6 kDa
- Amino acids
- 1 to 208
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
General info
- Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology (Probable). Recruits VPS13B to the Golgi membrane (PubMed:25492866). Regulates the compacted morphology of the Golgi (PubMed:26209634). Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells (PubMed:17707369). Plays a role in neuron projection development (PubMed:25492866).
- Sequence similarities
Belongs to the small GTPase superfamily. Rab family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RAB6B also known as ras-related protein Rab-6B is a small GTPase with a molecular weight of approximately 24 kDa. It plays a mechanical role in intracellular trafficking processes specifically in the regulation of vesicular transport and Golgi apparatus function. RAB6B is widely expressed in human tissues with heightened levels observed in brain tissue particularly in cerebral cortex neurons and the central nervous system. This makes it important for understanding neurological processes and maintaining cellular homeostasis in such tissues.
- Biological function summary
RAB6B acts in coordination with components of the vesicle transport machinery. It is not part of a large protein complex but interacts with several regulatory proteins to ensure accurate vesicle movement and docking within cells. This protein mainly influences retrograde transport from the Golgi back to the endoplasmic reticulum. This activity supports the recycling of proteins and lipids aiding in the proper maintenance of the Golgi structure and overall cellular function.
- Pathways
RAB6B modulates significant pathways related to membrane trafficking. It has pronounced involvement in the retrograde vesicle transport pathway impacting the transport of cargos in neurons. RAB6B's interaction with other RAB proteins especially with members such as RAB3 and RAB8 influences synaptic vesicle exocytosis and endosomal sorting. This highlights its role in neurotransmitter release and synaptic plasticity which are important for neuronal communication.
- Associated diseases and disorders
Abnormalities in RAB6B expression and function associate with neurological disorders particularly Alzheimer's disease and Autism Spectrum Disorder. In Alzheimer's RAB6B's interaction with amyloid precursor protein (APP) influences amyloid-beta peptide generation an important event in disease pathology. In autism altered RAB6B activities potentially affect synaptic vesicle trafficking leading to impaired neural connectivity and function. Understanding these connections can provide deeper insights into the mechanisms behind these conditions.
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1 product image
SDS-PAGE - Recombinant Human RAB6B protein (ab104035)
15% SDS-PAGE analysis of ab104035 (3 μg).
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