Recombinant Human RAB7 protein is a Human Full Length protein, in the 1 to 207 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
M G S S H H H H H H S S G L V P R G S H M T S R K K V L L K V I I L G D S G V G K T S L M N Q Y V N K K F S N Q Y K A T I G A D F L T K E V M V D D R L V T M Q I W D T A G Q E R F Q S L G V A F Y R G A D C C V L V F D V T A P N T F K T L D S W R D E F L I Q A S P R D P E N F P F V V L G N K I D L E N R Q V A T K R A Q A W C Y S K N N I P Y F E T S A K E A I N V E Q A F Q T I A R N A L K Q E T E V E L Y N E F P E P I K L D K N D R A K A S A E S C S C
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Required for vesicular trafficking and cell surface expression of ACE2 (PubMed:33147445). May play a role in PRPH neuronal intermediate filament assembly (By similarity).
RAB7, RAB7, RAB7A, Ras-related protein Rab-7a
Recombinant Human RAB7 protein is a Human Full Length protein, in the 1 to 207 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE, MS
No
No
Human
pH: 8
Constituents: 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
M G S S H H H H H H S S G L V P R G S H M T S R K K V L L K V I I L G D S G V G K T S L M N Q Y V N K K F S N Q Y K A T I G A D F L T K E V M V D D R L V T M Q I W D T A G Q E R F Q S L G V A F Y R G A D C C V L V F D V T A P N T F K T L D S W R D E F L I Q A S P R D P E N F P F V V L G N K I D L E N R Q V A T K R A Q A W C Y S K N N I P Y F E T S A K E A I N V E Q A F Q T I A R N A L K Q E T E V E L Y N E F P E P I K L D K N D R A K A S A E S C S C
Full Length
25.6 kDa
1 to 207
Recombinant
His tag N-Terminus
Liquid
purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA (PubMed:11179213, PubMed:12944476, PubMed:14617358, PubMed:20028791, PubMed:21255211). Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). Required for vesicular trafficking and cell surface expression of ACE2 (PubMed:33147445). May play a role in PRPH neuronal intermediate filament assembly (By similarity).
Belongs to the small GTPase superfamily. Rab family.
Deubiquitination at Lys-191 and Lys-194 by USP32.
Late endosome membrane, Lysosome membrane, Endosome membrane
Blue Ice
-20°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This supplementary information is collated from multiple sources and compiled automatically.
RAB7 also known as RAB7A is a small GTPase belonging to the RAS oncogene family. It has a molecular mass of approximately 23 kDa. This protein plays an important role in the regulation of late endocytic trafficking. It is prominently expressed in the cytoplasmic compartments of various cell types and serves as an important late endosome marker. RAB7 controls the maturation of early endosomes into late endosomes and their subsequent fusion with lysosomes therefore influencing degradation pathways.
RAB7 affects various processes including autophagy cell signaling and pathogen infection response. It often functions as part of the endosomal machinery and forms complexes with other proteins like RAB3GAP1 and RILP. These complexes help mediate vesicle transport and membrane trafficking processes which are essential for the maintenance of cellular homeostasis. RAB7 is significant in ensuring proper lysosomal positioning and function which is critical for cellular metabolism and degradation.
RAB7 integrates into the endocytic and autophagic pathways by facilitating the transport between endosomes and lysosomes. It connects with the PI3K/AKT pathway impacting cell proliferation and survival. RAB7 also interacts with the retromer complex influencing the retrieval of receptors from endosomes. Proteins like FYCO1 and Prostaglandin E2 synthase can also cooperate with RAB7 in these pathways further establishing its role in cellular dynamics and signaling.
RAB7 has associations with Charcot-Marie-Tooth disease type 2B and cancer progression. Mutations in the RAB7 gene have been linked to neuropathic conditions leading to impaired motor and sensory function. In the context of cancer dysregulation of RAB7 expression can contribute to increased tumor growth and metastasis. RAB7 interacts with proteins such as MAPK and ARL8B which are involved in these diseases highlighting its importance in pathological conditions.
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3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.
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