Recombinant Human Rad17 protein is a Human Fragment protein, in the 1 to 110 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
Amino acid sequence
M N Q V T D W V D P S F D D F L E C S G V S T I T A T S L G V N N S S H R R K N G P S T L E S S R F P A R K R G N L S S L E Q I Y G L E N S K E Y L S E N E P W V D K Y K P E T Q H E L A V H K K K I E E V E T W L K A Q V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Alternative names
Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, R24L, RAD17
Recommended products
Recombinant Human Rad17 protein is a Human Fragment protein, in the 1 to 110 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Alternative names
Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog, R24L, RAD17
Key facts
- Expression system
Wheat germ
- Tags
GST tag N-Terminus
- Applications
ELISA, WB
- Biologically active
No
- Accession
- O75943-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
M N Q V T D W V D P S F D D F L E C S G V S T I T A T S L G V N N S S H R R K N G P S T L E S S R F P A R K R G N L S S L E Q I Y G L E N S K E Y L S E N E P W V D K Y K P E T Q H E L A V H K K K I E E V E T W L K A Q V
- Accession
- Protein length
Fragment
- Amino acids
1 to 110
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
- Sequence similarities
Belongs to the rad17/RAD24 family.
- Post-translational modifications
Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
U+00A0;
U+00A0;
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rad17 also known as CCYC1 is a protein involved in DNA damage checkpoint control. It has an approximate molecular weight of 75 kDa. Rad17 is expressed in various tissues with higher levels in proliferating cells such as cancerous tissues. It functions as a part of the replication factor C (RFC) complex delivering its essential role during the DNA replication and damage response.
- Biological function summary
Rad17 plays an important role in maintaining genome stability by activating cell cycle checkpoints. It belongs to the RFC-like complex which identifies DNA damage and initiates the DNA repair process. The complex binds to chromatin and helps recruit the 9-1-1 complex (Rad9-Rad1-Hus1) to sites of DNA damage. This action is important for the cell to halt the cell cycle and commence repair preventing mutations from being passed on during cell division.
- Pathways
Rad17 functions in the DNA damage response pathway and is involved in the ATR-Chk1 pathway. Rad17 recruits ATR (ataxia-telangiectasia and Rad3-related protein) to stalled replication forks facilitating the phosphorylation and activation of Chk1 a checkpoint kinase. This signal allows cells to pause the replication process and repair DNA before continuing the cell cycle. Rad17 is closely related to proteins such as ATR and Chk1 through these pathways as they work together to maintain cellular integrity.
- Associated diseases and disorders
Rad17 has links to cancer and genomic instability syndromes. Mutations or malfunctions in Rad17 can disrupt normal checkpoint control and repair mechanisms leading to uncontrolled cell proliferation and tumor development. In cancer Rad17 interacts with proteins such as BRCA1 in pathways that emphasize DNA repair and checkpoint activation. BRCA1 and Rad17 coordinate to preserve genomic stability and disruptions in these proteins' functions can contribute to the onset of certain breast and ovarian cancers.
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1 product image
SDS-PAGE - Recombinant Human Rad17 protein (ab159302)
ab159302 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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