Recombinant Human Rad51D protein is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M G V L R V G L C P G L T E E M I Q L L R S H R I K T V V D L V S A D L E E V A Q K C G L S Y K A L V A L R R V L L A Q F S A F P V N G A D L Y E E L K T S T A I L S T G I G S L D K L L D A G L Y T G E V T E I V G G P G S G K T Q V C L C M A A N V A H G L Q Q N V L Y V D S N G G L T A S R L L Q L L Q A K T Q D E E E Q A E A L R R I Q V V H A F D I F Q M L D V L Q E L R G T V A Q Q V T G S S G T V K V V V V D S V T A V V S P L L G G Q Q R E G L A L M M Q L A R E L K T L A R D L G M A V V V T N H I T R D R D S G R L K P A L G R S W S F V P S T R I L L D T I E G A G A S G G R R M A C L A K S S R Q P T G F Q E M V D I G T W G T S E Q S A T L Q G D Q T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Alternative names
RAD51L3, RAD51D, DNA repair protein RAD51 homolog 4, R51H3, RAD51 homolog D, RAD51-like protein 3, TRAD
Recommended products
Recombinant Human Rad51D protein is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- O75771-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M G V L R V G L C P G L T E E M I Q L L R S H R I K T V V D L V S A D L E E V A Q K C G L S Y K A L V A L R R V L L A Q F S A F P V N G A D L Y E E L K T S T A I L S T G I G S L D K L L D A G L Y T G E V T E I V G G P G S G K T Q V C L C M A A N V A H G L Q Q N V L Y V D S N G G L T A S R L L Q L L Q A K T Q D E E E Q A E A L R R I Q V V H A F D I F Q M L D V L Q E L R G T V A Q Q V T G S S G T V K V V V V D S V T A V V S P L L G G Q Q R E G L A L M M Q L A R E L K T L A R D L G M A V V V T N H I T R D R D S G R L K P A L G R S W S F V P S T R I L L D T I E G A G A S G G R R M A C L A K S S R Q P T G F Q E M V D I G T W G T S E Q S A T L Q G D Q T
- Accession
- O75771
- Protein length
- Full Length
- Predicted molecular weight
- 37.4 kDa
- Amino acids
- 1 to 328
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab177590 was purified using conventional chromatography techniques.
General info
- Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
- Sequence similarities
Belongs to the RecA family. RAD51 subfamily.
- Subcellular localisation
- Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rad51D also known as RAD51L3 is a protein involved in homologous recombination repair of DNA. It has a molecular weight of approximately 37 kDa. Rad51D is primarily expressed in tissues with high cell division rates such as the testis and thymus. This protein belongs to the RAD51 family which is responsible for repairing DNA double-strand breaks (DSBs) that can arise during replication or after exposure to genotoxic agents. Rad51D's role centers on promoting the exchange of DNA strands during repair processes which helps maintain genome stability.
- Biological function summary
Rad51D plays a significant role in maintaining genomic integrity by facilitating homologous recombination. It interacts with Rad51 paralogs like Rad51C as part of the BCDX2 complex which also includes Rad51B and XRCC2. This complex is essential in the early steps of homologous recombination especially in forming the synaptic complex that searches for homology. The interactions among these proteins enable efficient strand invasion and exchange essential for error-free DNA repair.
- Pathways
Rad51D is an important component in DNA repair and damage response pathways. It primarily functions within the homologous recombination repair pathway. In this pathway Rad51D works closely with the RAD52 epistasis group including proteins such as BRCA1 and BRCA2 which assist in the proper localization and functioning of RAD51 recombinase. Rad51D's involvement in these pathways ensures accurate and effective DNA repair preventing genomic instability that could lead to cellular dysfunction.
- Associated diseases and disorders
Rad51D mutations have been linked to a higher risk of breast and ovarian cancer. Its association with the BRCA1 and BRCA2 proteins suggests a shared pathway in hereditary breast and ovarian cancer syndromes where deficient DNA repair leads to genomic mutations and tumorigenesis. Further research continues to explore Rad51D's potential as a biomarker for cancer susceptibility and its utility in developing targeted therapies.
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SDS-PAGE - Recombinant Human Rad51D protein (ab177590)
15% SDS-PAGE analysis of ab177590 (3μg).
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