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Recombinant Human Rad6 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Alternative names=Ubiquitin-conjugating enzyme E2 A, E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, HR6A, hHR6A, UBE2A, RAD6A
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M S T P A R R R L M R D F K R L Q E D P P A G V S G A P S E N N I M V W N A V I F G P E G T P F E D G T F K L T I E F T E E Y P N K P P T V R F V S K M F H P N V Y A D G S I C L D I L Q N R W S P T Y D V S S I L T S I Q S L L D E P N P N S P A N S Q A A Q L Y Q E N K R E Y E K R V S A I V E Q S W R D C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant Human Rad6 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Alternative names=Ubiquitin-conjugating enzyme E2 A, E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, HR6A, hHR6A, UBE2A, RAD6A
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M G S S H H H H H H S S G L V P R G S H M S T P A R R R L M R D F K R L Q E D P P A G V S G A P S E N N I M V W N A V I F G P E G T P F E D G T F K L T I E F T E E Y P N K P P T V R F V S K M F H P N V Y A D G S I C L D I L Q N R W S P T Y D V S S I L T S I Q S L L D E P N P N S P A N S Q A A Q L Y Q E N K R E Y E K R V S A I V E Q S W R D C
- Accession
- P49459-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
Purified by using conventional chromatography techniques
General info
- Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA.
- Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
- Post-translational modifications
Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Product promise
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