Recombinant human Rad6 protein (Active)
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Recombinant human Rad6 protein (Active) is a Human Full Length protein, in the 1 to 152 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
View Alternative Names
RAD6A, UBE2A, Ubiquitin-conjugating enzyme E2 A, E2 ubiquitin-conjugating enzyme A, RAD6 homolog A, Ubiquitin carrier protein A, Ubiquitin-protein ligase A, HR6A, hHR6A
- FuncS
Supplier Data
Functional Studies - Recombinant human Rad6 protein (Active) (AB269094)
The specific activity of ab269094 was 30 nmol/min/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant human Rad6 protein (Active) (AB269094)
SDS-PAGE analysis of ab269094.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rad6 functions as part of a larger protein complex involved in the DNA damage repair process. It collaborates with the RAD6 epistasis group which ensures the maintenance of genomic stability. Rad6 as a ubiquitin-conjugating enzyme interacts with E3 ubiquitin ligases facilitating the ubiquitination of target proteins that require degradation or regulation in response to DNA damage. This activity is vital for DNA repair mechanisms like post-replication repair (PRR) which protects cells from mutagenic events.
Pathways
Rad6 integrates into critical biological processes including the ubiquitin-proteasome pathway and the DNA repair pathway. Within the ubiquitin-proteasome system Rad6 aids in tagging proteins for degradation thereby regulating protein levels and cellular functions. It connects to other proteins like E3 enzymes and RAD18 in the DNA repair pathway. Its activity in DNA repair ensures the appropriate response to DNA damage coordinating with other mechanistic proteins to maintain genomic stability.
Specifications
Form
Liquid
General info
Function
E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin-protein ligase (PubMed : 16337599, PubMed : 20061386, PubMed : 23685073, PubMed : 25582440, PubMed : 38297121). In vitro catalyzes 'Lys-11', as well as 'Lys-48'-linked polyubiquitination (PubMed : 20061386). Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1 (PubMed : 16337599). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation (PubMed : 16337599). Involved in mitophagy by acting as a E2 ubiquitin-conjugating enzyme for PRKN (PubMed : 23685073). In association with the E3 enzyme UBR4, is involved in N-end rule-dependent protein degradation (PubMed : 38182926). In association with the E3 ubiquitin-protein ligase complex SIFI, inhibits the mitochondrial stress response by acting as a E2 ubiquitin-conjugating enzyme for UBR4 and KCMF1 (PubMed : 38297121).
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family.
Post-translational modifications
Phosphorylation at Ser-120 by CDK9 increases activity towards histone H2B.
Target data
Complementary Products
Product promise
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