Recombinant Human Rag A protein
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Recombinant Human Rag A protein is a Human Full Length protein, in the 1 to 313 aa range, expressed in Wheat germ, suitable for ELISA, WB.
View Alternative Names
Ras-related GTP-binding protein A, Rag A, RagA, Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1, RRAGA
- SDS-PAGE
Unknown
SDS-PAGE - Recombinant Human Rag A protein (AB161103)
ab161103 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Reactivity data
Product details
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rag A interacts with amino acids and nutrient signaling by activating the mTORC1 complex a central regulator of cell growth. It ensures correct localization of mTORC1 to the lysosomal surface facilitating its activation. The mTORC1 complex includes other proteins such as RAPTOR and mLST8 which collectively modulate cell metabolism and protein synthesis. Rag A's participation is essential for sensing nutrients and the energy state within cells.
Pathways
With regards to pathways Rag A is involved in the mTOR signaling pathway and the autophagy-lysosome pathway. These two pathways are important to maintaining cellular homeostasis and responding to nutrient availability. Rag A directly interacts with Rag C another protein in this pathway to relay the presence of amino acids to mTORC1. Through these interactions Rag A aids in the regulation of cellular growth energy management and metabolism in response to environmental cues.
Specifications
Form
Liquid
General info
Function
Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed : 20381137, PubMed : 24095279, PubMed : 25936802, PubMed : 31601708, PubMed : 31601764, PubMed : 38103557). Forms heterodimeric Rag complexes with RagC/RRAGC or RagD/RRAGD and cycles between an inactive GDP-bound and an active GTP-bound form : RagA/RRAGA is in its active form when GTP-bound RagA/RRAGA forms a complex with GDP-bound RagC/RRAGC (or RagD/RRAGD) and in an inactive form when GDP-bound RagA/RRAGA heterodimerizes with GTP-bound RagC/RRAGC (or RagD/RRAGD) (PubMed : 20381137, PubMed : 24095279, PubMed : 25936802, PubMed : 31601708, PubMed : 31601764, PubMed : 32868926). In its GTP-bound active form, promotes the recruitment of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB (PubMed : 20381137, PubMed : 25936802, PubMed : 31601708, PubMed : 31601764). Involved in the RCC1/Ran-GTPase pathway (PubMed : 9394008). May play a direct role in a TNF-alpha signaling pathway leading to induction of cell death (PubMed : 8995684).. (Microbial infection) May alternatively act as a cellular target for adenovirus E3-14.7K, an inhibitor of TNF-alpha functions, thereby affecting cell death.
Sequence similarities
Belongs to the GTR/RAG GTP-binding protein family.
Post-translational modifications
Polybiquitinated via 'Lys-63'-linked polyubiquitination by RNF152 in response to amino acid starvation: polyubiquitination of the GDP-bound inactive form by RNF152 promotes RRAGA inactivation and interaction with the GATOR1 complex (PubMed:25936802). This does not affect RRAGA degradation (PubMed:25936802).
Subcellular localisation
Nucleus
Target data
Complementary Products
Product promise
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