Recombinant Human RALA protein is a Human Full Length protein, in the 1 to 203 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, WB.
Images
Publications
Filter by
- Cell 168:878-889.e292017Multivalent Small-Molecule Pan-RAS Inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew E Welsch,Anna Kaplan,Jennifer M Chambers,Michael E Stokes,Pieter H Bos,Arie Zask,Yan Zhang,Marta Sanchez-Martin,Michael A Badgley,Christine S Huang,Timothy H Tran,Hemanth Akkiraju,Lewis M Brown,Renu Nandakumar,Serge Cremers,Wan Seok Yang,Liang Tong,Kenneth P Olive,Adolfo Ferrando,Brent R StockwellPubMed 28235199
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, WB
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S H M A A N K P K G Q N S L A L H K V I M V G S G G V G K S A L T L Q F M Y D E F V E D Y E P T K A D S Y R K K V V L D G E E V Q I D I L D T A G Q E D Y A A I R D N Y F R S G E G F L C V F S I T E M E S F A A T A D F R E Q I L R V K E D E N V P F L L V G N K S D L E D K R Q V S V E E A K N R A E Q W N V N Y V E T S A K T R A N V D K V F F D L M R E I R A R K M E D S K E K N G K K K R K S L A K R I R E R C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab102555 can be used as a WB positive control in conjunction with ab96759. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors (PubMed:18756269, PubMed:19306925, PubMed:20005108, PubMed:21822277, PubMed:30500825). Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (PubMed:19306925). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (PubMed:18756269). During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1 (PubMed:21822277).
Alternative names
RAL, RALA, Ras-related protein Ral-A
Recommended products
Recombinant Human RALA protein is a Human Full Length protein, in the 1 to 203 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS, WB.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS, WB
- Biologically active
- No
- Accession
- P11233-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S H M A A N K P K G Q N S L A L H K V I M V G S G G V G K S A L T L Q F M Y D E F V E D Y E P T K A D S Y R K K V V L D G E E V Q I D I L D T A G Q E D Y A A I R D N Y F R S G E G F L C V F S I T E M E S F A A T A D F R E Q I L R V K E D E N V P F L L V G N K S D L E D K R Q V S V E E A K N R A E Q W N V N Y V E T S A K T R A N V D K V F F D L M R E I R A R K M E D S K E K N G K K K R K S L A K R I R E R C
- Accession
- P11233
- Protein length
- Full Length
- Predicted molecular weight
- 25.8 kDa
- Amino acids
- 1 to 203
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab102555 was purified using conventional chromatography techniques.
General info
- Function
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors (PubMed:18756269, PubMed:19306925, PubMed:20005108, PubMed:21822277, PubMed:30500825). Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. The RALA-exocyst complex regulates integrin-dependent membrane raft exocytosis and growth signaling (PubMed:20005108). Key regulator of LPAR1 signaling and competes with GRK2 for binding to LPAR1 thus affecting the signaling properties of the receptor. Required for anchorage-independent proliferation of transformed cells (PubMed:19306925). During mitosis, supports the stabilization and elongation of the intracellular bridge between dividing cells. Cooperates with EXOC2 to recruit other components of the exocyst to the early midbody (PubMed:18756269). During mitosis, also controls mitochondrial fission by recruiting to the mitochondrion RALBP1, which mediates the phosphorylation and activation of DNM1L by the mitotic kinase cyclin B-CDK1 (PubMed:21822277).
- Sequence similarities
Belongs to the small GTPase superfamily. Ras family.
- Post-translational modifications
Phosphorylated. Phosphorylation at Ser-194 by AURKA/Aurora kinase A, during mitosis, induces RALA localization to the mitochondrion where it regulates mitochondrial fission.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RALA also known as Ras-related protein Ral-A or RalA is a small GTPase involved in a variety of cellular processes. It has a molecular mass of approximately 23 kDa. RALA switches between inactive GDP-bound and active GTP-bound states facilitating the transduction of signals within the cell. This protein is found in a range of tissues with expression in the brain lung and kidney being notable. The ability of RALA to shuttle between membrane compartments highlights its mechanical versatility in cellular signaling.
- Biological function summary
The interaction of RALA with effector proteins drives key processes like vesicle trafficking cytoskeletal dynamics and gene expression. RALA forms part of a signaling complex in its active state that mediates these functions. This protein is vital in the neurotransmitter release ensuring efficient communication between neurons. Additionally RALA impacts cell movement by influencing actin filament organization showing its role in cell motility.
- Pathways
The engagement of RALA in Ral signaling and the phosphatidylinositol 3-kinase (PI3K) pathway showcases its importance. In Ral signaling RALA interacts closely with proteins like RalBP1 influencing endocytosis and exocytosis activities. In the PI3K pathway RALA helps regulate cell proliferation and survival showing interactions with proteins such as AKT1. These pathways highlight the integration of RALA into broad signal transduction networks important for cellular homeostasis.
- Associated diseases and disorders
The altered regulation of RALA links it to cancer and neuronal disorders. In cancer the dysregulation of RALA can drive tumorigenesis often acting alongside proteins like KRAS in promoting oncogenic signaling. In neuronal disorders improper functioning of RALA has been associated with synaptic dysfunctions potentially affecting proteins like Synapsin I which are important to synaptic vesicle trafficking. Understanding RALA's role in these contexts helps in developing targeted therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant Human RALA protein (ab102555)
15% SDS-PAGE analysis of 3μg of ab102555.
Western blot - Recombinant Human RALA protein (ab102555)
ab96759 recognizes the full length tagged recombinant protein ab102555 which has an expected molecular weight of 30 kDa.
All lanes: Anti-RALA antibody (ab96759) at 1/3000 dilution
All lanes: Western blot - Recombinant Human RALA protein (ab102555) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 4min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com