Recombinant Human RanGAP1 protein is a Human Full Length protein, in the 1 to 587 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M A S E D I A K L A E T L A K T Q V A G G Q L S F K G K S L K L N T A E D A K D V I K E I E D F D S L E A L R L E G N T V G V E A A R V I A K A L E K K S E L K R C H W S D M F T G R L R T E I P P A L I S L G E G L I T A G A Q L V E L D L S D N A F G P D G V Q G F E A L L K S S A C F T L Q E L K L N N C G M G I G G G K I L A A A L T E C H R K S S A Q G K P L A L K V F V A G R N R L E N D G A T A L A E A F R V I G T L E E V H M P Q N G I N H P G I T A L A Q A F A V N P L L R V I N L N D N T F T E K G A V A M A E T L K T L R Q V E V I N F G D C L V R S K G A V A I A D A I R G G L P K L K E L N L S F C E I K R D A A L A V A E A M A D K A E L E K L D L N G N T L G E E G C E Q L Q E V L E G F N M A K V L A S L S D D E D E E E E E E G E E E E E E A E E E E E E D E E E E E E E E E E E E E E P Q Q R G Q G E K S A T P S R K I L D P N T G E P A P V L S S P P P A D V S T F L A F P S P E K L L R L G P K S S V L I A Q Q T D T S D P E K V V S A F L K V S S V F K D E A T V R M A V Q D A V D A L M Q K A F N S S S F N S N T F L T R L L V H M G L L K S E D K V K A I A N L Y G P L M A L N H M V Q Q D Y F P K A L A P L L L A F V T K P N S A L E S C S F A R H S L L Q T L Y K V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes (Recombinant protein). |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
GTPase activator for RAN (PubMed:16428860, PubMed:8146159, PubMed:8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:27160050, PubMed:8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).
Alternative names
KIAA1835, SD, RANGAP1, Ran GTPase-activating protein 1, RanGAP1
Recommended products
Recombinant Human RanGAP1 protein is a Human Full Length protein, in the 1 to 587 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- Tag free
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P46060-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M A S E D I A K L A E T L A K T Q V A G G Q L S F K G K S L K L N T A E D A K D V I K E I E D F D S L E A L R L E G N T V G V E A A R V I A K A L E K K S E L K R C H W S D M F T G R L R T E I P P A L I S L G E G L I T A G A Q L V E L D L S D N A F G P D G V Q G F E A L L K S S A C F T L Q E L K L N N C G M G I G G G K I L A A A L T E C H R K S S A Q G K P L A L K V F V A G R N R L E N D G A T A L A E A F R V I G T L E E V H M P Q N G I N H P G I T A L A Q A F A V N P L L R V I N L N D N T F T E K G A V A M A E T L K T L R Q V E V I N F G D C L V R S K G A V A I A D A I R G G L P K L K E L N L S F C E I K R D A A L A V A E A M A D K A E L E K L D L N G N T L G E E G C E Q L Q E V L E G F N M A K V L A S L S D D E D E E E E E E G E E E E E E A E E E E E E D E E E E E E E E E E E E E E P Q Q R G Q G E K S A T P S R K I L D P N T G E P A P V L S S P P P A D V S T F L A F P S P E K L L R L G P K S S V L I A Q Q T D T S D P E K V V S A F L K V S S V F K D E A T V R M A V Q D A V D A L M Q K A F N S S S F N S N T F L T R L L V H M G L L K S E D K V K A I A N L Y G P L M A L N H M V Q Q D Y F P K A L A P L L L A F V T K P N S A L E S C S F A R H S L L Q T L Y K V
- Accession
- P46060
- Protein length
- Full Length
- Predicted molecular weight
- 90.31 kDa
- Amino acids
- 1 to 587
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
GTPase activator for RAN (PubMed:16428860, PubMed:8146159, PubMed:8896452). Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export (PubMed:27160050, PubMed:8896452). Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export (PubMed:27160050).
- Sequence similarities
Belongs to the RNA1 family.
- Post-translational modifications
Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis.
- Subcellular localisation
- Nucleus, Nucleoplasm, Nucleus envelope, Cytoskeleton, Spindle
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RanGAP1 also known as Ran GTPase-activating protein 1 plays an important role in regulating nucleocytoplasmic transport. Mechanically it converts RanGTP to RanGDP in the cytoplasm which is essential for the recycling of Ran and maintenance of the Ran gradient across the nuclear envelope. The molecular mass of RanGAP1 is approximately 70 kDa. RanGAP1 is expressed in various cell types with significant presence in the cytoplasm due to its function.
- Biological function summary
Many cellular processes rely on the action of RanGAP1 as it forms part of the Ran cycle. This protein is an integral component of the nuclear transport complex which regulates the bidirectional movement of RNA and proteins through the nuclear pores. Through its action RanGAP1 indirectly influences nuclear import and export playing an essential part in cell cycle regulation and mitosis.
- Pathways
Several key cellular pathways are dependent on RanGAP1 activity. For instance it is a component of the mitotic spindle assembly pathway and also plays a role in the RNA processing pathway. In these pathways RanGAP1 works closely with other proteins such as RanBP1 and RCC1 facilitating important cellular events that require strict regulation of nuclear-cytoplasmic transport.
- Associated diseases and disorders
Disruptions in RanGAP1 function have been associated with certain types of cancer and neurodegenerative diseases. Aberrant regulation of RanGAP1 activity can lead to improper cell cycle progression contributing to oncogenesis. Furthermore changes in the expression of RanGAP1 and its interacting proteins like RanBP2 may relate to disorders like Huntington's disease where cellular transport mechanisms are compromised.
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1 product image
SDS-PAGE - Recombinant Human RanGAP1 protein (ab131750)
12.5% SDS-PAGE analysis of ab131750, stained with Coomassie Blue.
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