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Recombinant human RANKL protein (Active) is a Human Full Length protein, in the 143 to 317 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

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Key facts

Purity
>=98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Escherichia coli
Tags
Tag free
Applications
SDS-PAGE, FuncS, HPLC
Biologically active
Yes

Amino acid sequence

M E K A M V D G S W L D L A K R S K L E A Q P F A H L T I N A T D I P S G S H K V S L S S W Y H D R G W A K I S N M T F S N G K L I V N Q D G F Y Y L Y A N I C F R H H E T S G D L A T E Y L Q L M V Y V T K T S I K I P S S H T L M K G G S T K Y W S G N S E F H F Y S I N V G G F F K L R S G E E I S I E V S N P S L L D P D Q D A T Y F G A F K V R D I D

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
FuncS
Reactivity
Reacts
Dilution info
-
Notes

-

Application
HPLC
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

Alternative names

Recommended products

Recombinant human RANKL protein (Active) is a Human Full Length protein, in the 143 to 317 aa range, expressed in Escherichia coli, with >=98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, HPLC.

Key facts

Purity
>=98% SDS-PAGE
Endotoxin level
< 1 EU/µg
Expression system
Escherichia coli
Applications
SDS-PAGE, FuncS, HPLC
Biological activity
Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells.
Accession
O14788-1
Animal free
No
Carrier free
Yes
Species
Human
Concentration
Loading...
Storage buffer

Constituents: 0.435% Sodium chloride, 0.082% Sodium phosphate

Sequence info

Amino acid sequence

M E K A M V D G S W L D L A K R S K L E A Q P F A H L T I N A T D I P S G S H K V S L S S W Y H D R G W A K I S N M T F S N G K L I V N Q D G F Y Y L Y A N I C F R H H E T S G D L A T E Y L Q L M V Y V T K T S I K I P S S H T L M K G G S T K Y W S G N S E F H F Y S I N V G G F F K L R S G E E I S I E V S N P S L L D P D Q D A T Y F G A F K V R D I D
Accession
O14788
Protein length
Full Length
Predicted molecular weight
20 kDa
Amino acids
143 to 317
Nature
Recombinant

Specifications

Form
Lyophilized
Additional notes

>=98% HPLC analyses.Sterile filtered.

General info

Function

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (PubMed:22664871). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (By similarity).

Sequence similarities

Belongs to the tumor necrosis factor family.

Post-translational modifications

The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

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