Recombinant Human RAP1A protein is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE, MS.
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Publications
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e222542022ArhGEF12 activates Rap1A and not RhoA in human dermal microvascular endothelial cells to reduce tumor necrosis factor-induced leak.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlamzeb Khan,Weiming Ni,Tania Baltazar,Francesc Lopez-Giraldez,Jordan S Pober,Richard W PiercePubMed 35294066
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
< i > M G S S H H H H H H S S G L V P R G S H < / i > M R E Y K L V V L G S G G V G K S A L T V Q F V Q G I F V E K Y D P T I E D S Y R K Q V E V D C Q Q C M L E I L D T A G T E Q F T A M R D L Y M K N G Q G F A L V Y S I T A Q S T F N D L Q D L R E Q I L R V K D T E D V P M I L V G N K C D L E D E R V V G K E Q G Q N L A R Q W C N C A F L E S S A K S K I N V N E I F Y D L V R Q I N R K T P V E K K K P K K K S C
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed:17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
Alternative names
KREV1, RAP1A, Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1
Recommended products
Recombinant Human RAP1A protein is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >=85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >=85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P62834-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- < i > M G S S H H H H H H S S G L V P R G S H < / i > M R E Y K L V V L G S G G V G K S A L T V Q F V Q G I F V E K Y D P T I E D S Y R K Q V E V D C Q Q C M L E I L D T A G T E Q F T A M R D L Y M K N G Q G F A L V Y S I T A Q S T F N D L Q D L R E Q I L R V K D T E D V P M I L V G N K C D L E D E R V V G K E Q G Q N L A R Q W C N C A F L E S S A K S K I N V N E I F Y D L V R Q I N R K T P V E K K K P K K K S C
- Accession
- P62834
- Protein length
- Full Length
- Predicted molecular weight
- 22.8 kDa
- Amino acids
- 1 to 181
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab97904 is purified using conventional chromatography techniques.
General info
- Function
Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed:17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed:25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
- Sequence similarities
Belongs to the small GTPase superfamily. Ras family.
- Subcellular localisation
- Early endosome
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human RAP1A protein (ab97904)
15% SDS-PAGE showing ab97904 at approximately 22.8kDa (3μg).
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