JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB97904

Recombinant Human RAP1A protein (His tag N-Terminus)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human RAP1A protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 181 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KREV1, RAP1A, Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1

1 Images
SDS-PAGE - Recombinant Human RAP1A protein (His tag N-Terminus) (AB97904)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human RAP1A protein (His tag N-Terminus) (AB97904)

15% SDS-PAGE showing ab97904 at approximately 22.8kDa (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P62834

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 40% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Complementary Products

Sample Prep & Detection Kits

AB206996

Immunoprecipitation kit

Immunoprecipitation - Immunoprecipitation kit (AB206996)

  • 0
  • 0
Proteins & Peptides

AB85273

Recombinant Human 14-3-3 alpha + beta protein

SDS-PAGE - Recombinant Human 14-3-3 alpha + beta protein (AB85273)

  • 0
  • 0
Primary Antibodies

AB284792

Anti-RAP1A antibody [EP878] - BSA and Azide free

RabMAb

Western blot - Anti-RAP1A antibody [EP878] - BSA and Azide free (AB284792)

  • 0
  • 0
Proteins & Peptides

AB246002

Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active)

Electron Microscopy - Recombinant mouse Alpha-synuclein protein aggregate Type 1 (Active) (AB246002)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSC","proteinLength":"Full Length","predictedMolecularWeight":"22.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":181,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P62834","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab97904 is purified using conventional chromatography techniques.

style
left-column

General info

Function

Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed : 17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed : 25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Subcellular localisation

Early endosome

style
left-column

Product protocols

style
left-column

Target data

Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes (PubMed : 17916086). Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. Facilitates the progressive accumulation of CDH1 at mature desmosome junctions via cAMP-dependent signaling and its interaction with PKP3 (PubMed : 25208567). May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
See full target information RAP1A
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22254 PubMed35294066

2022

ArhGEF12 activates Rap1A and not RhoA in human dermal microvascular endothelial cells to reduce tumor necrosis factor-induced leak.

Applications

Unspecified application

Species

Unspecified reactive species

Alamzeb Khan,Weiming Ni,Tania Baltazar,Francesc Lopez-Giraldez,Jordan S Pober,Richard W Pierce
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com