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AB271499

Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active)

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Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) is a Human Full Length protein, in the 2 to 739 aa range, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

RBAP48, RBBP4, Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, Chromatin assembly factor I p48 subunit, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, Retinoblastoma-binding protein p48, CAF-1 subunit C, CAF-I 48 kDa subunit, CAF-I p48, RBBP-4

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Functional Studies - Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (AB271499)
  • FuncS

Unknown

Functional Studies - Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (AB271499)

Specific activity of ab271499 was 1.33 pmol/min/μg.

SDS-PAGE - Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (AB271499)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human RbAp48+EED+SUZ12+AEBP2+EZH2 (mutated A738T) protein (Active) (AB271499)

SDS-PAGE analysis of ab271499.

Key facts

Purity

>90% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 1.33 pmol/min/μg

Assay conditions: 50 μl reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 10 μM S-adenosylmethionine, and various amount of enzyme complex) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.

Accession

Q09028

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"APQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL","proteinLength":"Full Length","predictedMolecularWeight":"87 kDa","actualMolecularWeight":null,"aminoAcidEnd":739,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q15022","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"ADKEAAFDDAVEERVINEEYKIWKKNTPFLYDLVMTHALEWPSLTAQWLPDVTRPEGKDFSIHRLVLGTHTSDEQNHLVIASVQLPNDDAQFDASHYDSEKGEFGGFGSVSGKIEIEIKINHEGEVNRARYMPQNPCIIATKTPSSDVLVFDYTKHPSKPDPSGECNPDLRLRGHQKEGYGLSWNPNLSGHLLSASDDHTICLWDISAVPKEGKVVDAKTIFTGHTAVVEDVSWHLLHESLFGSVADDQKLMIWDTRSNNTSKPSHSVDAHTAEVNCLSFNPYSEFILATGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLNVWDLSKIGEEQSPEDAEDGPPELLFIHGGHTAKISDFSWNPNEPWVICSVSEDNIMQVWQMAENIYNDEDPEGSVDPEGQGS","proteinLength":"Full Length","predictedMolecularWeight":"48 kDa","actualMolecularWeight":null,"aminoAcidEnd":425,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q09028","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"SEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAKCTTLTHHKCGAAIRQTSFSRDSSILIAVCDDASIWRWDRLR","proteinLength":"Full Length","predictedMolecularWeight":"51 kDa","actualMolecularWeight":null,"aminoAcidEnd":441,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"O75530","tags":[{"tag":"DDDDK","terminus":"N-Terminus"}]},{"sequence":"AAAITDMADLEELSRLSPLPPGSPGSAARGRAEPPEEEEEEEEEEEEAEAEAVAALLLNGGSGGGGGGGGGGVGGGEAETMSEPSPESASQAGEDEDEEEDDEEEEDESSSSGGGEEESSAESLVGSSGGSSSDETRSLSPGAASSSSGDGDGKEGLEEPKGPRGSQGGGGGGSSSSSVVSSGGDEGYGTGGGGSSATSGGRRGSLEMSSDGEPLSRMDSEDSISSTIMDVDSTISSGRSTPAMMNGQGSTTSSSKNIAYNCCWDQCQACFNSSPDLADHIRSIHVDGQRGGVFVCLWKGCKVYNTPSTSQSWLQRHMLTHSGDKPFKCVVGGCNASFASQGGLARHVPTHFSQQNSSKVSSQPKAKEESPSKAGMNKRRKLKNKRRRSLPRPHDFFDAQTLDAIRHRAICFNLSAHIESLGKGHSVVFHSTVIAKRKEDSGKIKLLLHWMPEDILPDVWVNESERHQLKTKVVHLSKLPKDTALLLDPNIYRTMPQKRLKR","proteinLength":"Full Length","predictedMolecularWeight":"53 kDa","actualMolecularWeight":null,"aminoAcidEnd":503,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q6ZN18","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"GQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHRKCNYSFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP","proteinLength":"Full Length","predictedMolecularWeight":"86 kDa","actualMolecularWeight":null,"aminoAcidEnd":751,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q15910","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RbAp48 EED SUZ12 AEBP2 and EZH2 form a part of the Polycomb Repressive Complex 2 (PRC2) an important multiprotein complex involved in epigenetic regulation. This complex modulates chromatin structure and gene expression by methylating histone H3 on lysine 27 (H3K27me3). PRC2 requires proper assembly of its components to function including RbAp48 also known as Retinoblastoma binding protein 4 and EZH2 its core catalytic subunit. RbAp48 has a mass of about 48 kDa while EZH2 is about 85 kDa. PRC2 components express broadly in various tissues particularly in stem and progenitor cells which need tight gene expression control.
Biological function summary

RbAp48 EED SUZ12 AEBP2 and EZH2 enable transcriptional repression by modifying chromatin state to a transcriptionally silent one. They are integrated into the PRC2 complex essential for maintaining repression of genes involved in differentiation and development. PRC2 establishes silenced gene states dynamically especially important during early development and in maintaining stem cell pluripotency. The modification of chromatin by PRC2 strongly regulates developmental gene silencing impacting cell lineage decisions.

Pathways

The PRC2 complex and its activities contribute significantly to the maintenance of the closed chromatin state within the Wnt signaling pathway and the TGF-beta signaling pathway. In these pathways EZH2 and other PRC2 components interact with regulatory proteins and transcription factors to control gene expression essential for cell proliferation and differentiation. The interaction aligns with proteins like β-catenin and SMADs which depend on PRC2-mediated gene silencing to modulate signaling cascades effectively.

Aberrant function or expression of PRC2 complex components particularly EZH2 associates with several types of cancer such as prostate and breast cancer. Dysregulation leads to increased proliferation invasiveness and metastasis due to inappropriate gene repression influencing proteins like p16 and E2F. Altered activity of the PRC2 complex also links with neurodevelopmental disorders such as Weaver syndrome where mutations in EZH2 can disrupt normal developmental processes. In these cases the disrupted methylation patterns diverse developmental and cellular processes are affected highlighting the need for precise regulation by these proteins.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Sequence similarities

Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
See full target information RBBP4

Additional targets

EED,SUZ12,Zinc finger protein AEBP2,EZH2 mutated A738T
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