Recombinant human RBBP4 + EED + SUZ12 + AEBP2 + EZH2 (mutated P132S) protein (Active) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Sequence
This product is comprised of multiple sequences see
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
Additional Targets
SUZ12, AEBP2, RBBP4, EZH2 mutated P132S
Alternative names
Polycomb protein EED, hEED, Embryonic ectoderm development protein, WD protein associating with integrin cytoplasmic tails 1, WAIT-1, EED
Recommended products
Recombinant human RBBP4 + EED + SUZ12 + AEBP2 + EZH2 (mutated P132S) protein (Active) is a Human Full Length protein, in the 2 to 425 aa range, expressed in Baculovirus infected Sf9, with >=90% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- His tag N-Terminus DDDDK tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific Activity: 0.08 pmol/min/μg.
- Accession
- O75530-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Preservative: 1.36% Imidazole
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- Accession
- Q15910
- Protein length
- Full Length
- Predicted molecular weight
- 48 kDa
- Amino acids
- 2 to 425
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 2
- Accession
- O75530
- Protein length
- Full Length
- Predicted molecular weight
- 51 kDa
- Amino acids
- 2 to 441
- Nature
- Recombinant
- Tags
- DDDDK tag N-Terminus
Amino acid sequence 3
- Accession
- Q6ZN18
- Protein length
- Full Length
- Predicted molecular weight
- 53 kDa
- Amino acids
- 2 to 517
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 4
- Accession
- Q15910
- Protein length
- Full Length
- Predicted molecular weight
- 86 kDa
- Amino acids
- 2 to 746
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Amino acid sequence 5
- Accession
- Q15022
- Protein length
- Full Length
- Predicted molecular weight
- 87 kDa
- Amino acids
- 2 to 739
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
- Sequence similarities
Belongs to the WD repeat ESC family.
- Post-translational modifications
Methylated. Binding to histone H1 'Lys-26' promotes mono-, di-, and trimethylation of internal lysines.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Functional Studies - Recombinant human RBBP4 + EED + SUZ12 + AEBP2 + EZH2 (mutated P132S) protein (Active) (ab271504)
Specific activity of ab271504.
SDS-PAGE - Recombinant human RBBP4 + EED + SUZ12 + AEBP2 + EZH2 (mutated P132S) protein (Active) (ab271504)
SDS-PAGE analysis of 4.35 μg ab271504.
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