Recombinant human RBBP4 + EED + SUZ12 + EZH2 protein

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus DDDDK tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Biological activity

50 μl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 μM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K27 residue of histone H3, incubate 1 hour. Then incubate 1 hour with the HRP-labeled secondary antibody, followed by addition of the HRP substrate to produce chemiluminescence.

Accession

Q15910-1

Animal free

No

Species

Human

Concentration

Storage buffer

pH: 8
Preservative: 1.53% Imidazole
Constituents: 10% Glycerol (glycerin, glycerine), 0.71% Tris HCl, 0.64% Sodium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

Sequence info

Amino acid sequence 1

A D K E A A F D D A V E E R V I N E E Y K I W K K N T P F L Y D L V M T H A L E W P S L T A Q W L P D V T R P E G K D F S I H R L V L G T H T S D E Q N H L V I A S V Q L P N D D A Q F D A S H Y D S E K G E F G G F G S V S G K I E I E I K I N H E G E V N R A R Y M P Q N P C I I A T K T P S S D V L V F D Y T K H P S K P D P S G E C N P D L R L R G H Q K E G Y G L S W N P N L S G H L L S A S D D H T I C L W D I S A V P K E G K V V D A K T I F T G H T A V V E D V S W H L L H E S L F G S V A D D Q K L M I W D T R S N N T S K P S H S V D A H T A E V N C L S F N P Y S E F I L A T G S A D K T V A L W D L R N L K L K L H S F E S H K D E I F Q V Q W S P H N E T I L A S S G T D R R L N V W D L S K I G E E Q S P E D A E D G P P E L L F I H G G H T A K I S D F S W N P N E P W V I C S V S E D N I M Q V W Q M A E N I Y N D E D P E G S V D P E G Q G S

Accession

Q09028

Protein length

Full Length

Amino acids

2 to 425

Nature

Recombinant

Tags

His tag N-Terminus

Amino acid sequence 2

G Q T G K K S E K G P V C W R K R V K S E Y M R L R Q L K R F R R A D E V K S M F S S N R Q K I L E R T E I L N Q E W K Q R R I Q P V H I L T S V S S L R G T R E C S V T S D L D F P T Q V I P L K T L N A V A S V P I M Y S W S P L Q Q N F M V E D E T V L H N I P Y M G D E V L D Q D G T F I E E L I K N Y D G K V H G D R E C G F I N D E I F V E L V N A L G Q Y N D D D D D D D G D D P E E R E E K Q K D L E D H R D D K E S R P P R K F P S D K I F E A I S S M F P D K G T A E E L K E K Y K E L T E Q Q L P G A L P P E C T P N I D G P N A K S V Q R E Q S L H S F H T L F C R R C F K Y D C F L H R K C N Y S F H A T P N T Y K R K N T E T A L D N K P C G P Q C Y Q H L E G A K E F A A A L T A E R I K T P P K R P G G R R R G R L P N N S S R P S T P T I N V L E S K D T D S D R E A G T E T G G E N N D K E E E E K K D E T S S S S E A N S R C Q T P I K M K P N I E P P E N V E W S G A E A S M F R V L I G T Y Y D N F C A I A R L I G T K T C R Q V Y E F R V K E S S I I A P A P A E D V D T P P R K K K R K H R L W A A H C R K I Q L K K D G S S N H V Y N Y Q P C D H P R Q P C D S S C P C V I A Q N F C E K F C Q C S S E C Q N R F P G C R C K A Q C N T K Q C P C Y L A V R E C D P D L C L T C G A A D H W D S K N V S C K N C S I Q R G S K K H L L L A P S D V A G W G I F I K D P V Q K N E F I S E Y C G E I I S Q D E A D R R G K V Y D K Y M C S F L F N L N N D F V V D A T R K G N K I R F A N H S V N P N C Y A K V M M V N G D H R I G I F A K R A I Q T G E E L F F D Y R Y S Q A D A L K Y V G I E R E M E I P

Accession

Q15910

Protein length

Full Length

Amino acids

2 to 751

Nature

Recombinant

Tags

His tag N-Terminus

Amino acid sequence 3

A P Q K H G G G G G G G S G P S A G S G G G G F G G S A A V A A A T A S G G K S G G G S C G G G G S Y S A S S S S S A A A A A G A A V L P V K K P K M E H V Q A D H E L F L Q A F E K P T Q I Y R F L R T R N L I A P I F L H R T L T Y M S H R N S R T N I K R K T F K V D D M L S K V E K M K G E Q E S H S L S A H L Q L T F T G F F H K N D K P S P N S E N E Q N S V T L E V L L V K V C H K K R K D V S C P I R Q V P T G K K Q V P L N P D L N Q T K P G N F P S L A V S S N E F E P S N S H M V K S Y S L L F R V T R P G R R E F N G M I N G E T N E N I D V N E E L P A R R K R N R E D G E K T F V A Q M T V F D K N R R L Q L L D G E Y E V A M Q E M E E C P I S K K R A T W E T I L D G K R L P P F E T F S Q G P T L Q F T L R W T G E T N D K S T A P I A K P L A T R N S E S L H Q E N K P G S V K P T Q T I A V K E S L T T D L Q T R K E K D T P N E N R Q K L R I F Y Q F L Y N N N T R Q Q T E A R D D L H C P W C T L N C R K L Y S L L K H L K L C H S R F I F N Y V Y H P K G A R I D V S I N E C Y D G S Y A G N P Q D I H R Q P G F A F S R N G P V K R T P I T H I L V C R P K R T K A S M S E F L E S E D G E V E Q Q R T Y S S G H N R L Y F H S D T C L P L R P Q E M E V D S E D E K D P E W L R E K T I T Q I E E F S D V N E G E K E V M K L W N L H V M K H G F I A D N Q M N H A C M L F V E N Y G Q K I I K K N L C R N F M L H L V S M H D F N L I S I M S I D K A V T K L R E M Q Q K L E K G E S A S P A N E E I T E E Q N G T A N G F S E I N S K E K A L E T D S V S G V S K Q S K K Q K L

Accession

Q15022

Protein length

Full Length

Amino acids

2 to 739

Nature

Recombinant

Tags

His tag N-Terminus

Amino acid sequence 4

S E R E V S T A P A G T D M P A A K K Q K L S S D E N S N P D L S G D E N D D A V S I E S G T N T E R P D T P T N T P N A P G R K S W G K G K W K S K K C K Y S F K C V N S L K E D H N Q P L F G V Q F N W H S K E G D P L V F A T V G S N R V T L Y E C H S Q G E I R L L Q S Y V D A D A D E N F Y T C A W T Y D S N T S H P L L A V A G S R G I I R I I N P I T M Q C I K H Y V G H G N A I N E L K F H P R D P N L L L S V S K D H A L R L W N I Q T D T L V A I F G G V E G H R D E V L S A D Y D L L G E K I M S C G M D H S L K L W R I N S K R M M N A I K E S Y D Y N P N K T N R P F I S Q K I H F P D F S T R D I H R N Y V D C V R W L G D L I L S K S C E N A I V C W K P G K M E D D I D K I K P S E S N V T I L G R F D Y S Q C D I W Y M R F S M D F W Q K M L A L G N Q V G K L Y V W D L E V E D P H K A K C T T L T H H K C G A A I R Q T S F S R D S S I L I A V C D D A S I W R W D R L R

Accession

O75530

Protein length

Full Length

Amino acids

2 to 441

Nature

Recombinant

Tags

DDDDK tag N-Terminus

Product promise