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AB268918

Recombinant Human RbBP5 protein (His tag)

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(1 Publication)

Recombinant Human RbBP5 protein (His tag) is a Human Full Length protein, in the 1 to 538 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

View Alternative Names

RBQ3, RBBP5, Retinoblastoma-binding protein 5, RBBP-5, Retinoblastoma-binding protein RBQ-3

1 Images
SDS-PAGE - Recombinant Human RbBP5 protein (His tag) (AB268918)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human RbBP5 protein (His tag) (AB268918)

SDS-PAGE analysis of ab268918.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Q15291

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Complementary Products

Proteins & Peptides

AB123224

Recombinant E. coli SSB protein

SDS-PAGE - Recombinant E. coli SSB protein (AB123224)

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Primary Antibodies

AB154755

Anti-RbBP5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RbBP5 antibody (AB154755)

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Proteins & Peptides

AB98079

Recombinant Human WDR5 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human WDR5 protein (His tag N-Terminus) (AB98079)

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Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"linker":null,"sequence":"MNLELLESFGQNYPEEADGTLDCISMALTCTFNRWGTLLAVGCNDGRIVIWDFLTRGIAKIISAHIHPVCSLCWSRDGHKLVSASTDNIVSQWDVLSGDCDQRFRFPSPILKVQYHPRDQNKVLVCPMKSAPVMLTLSDSKHVVLPVDDDSDLNVVASFDRRGEYIYTGNAKGKILVLKTDSQDLVASFRVTTGTSNTTAIKSIEFARKGSCFLINTADRIIRVYDGREILTCGRDGEPEPMQKLQDLVNRTPWKKCCFSGDGEYIVAGSARQHALYIWEKSIGNLVKILHGTRGELLLDVAWHPVRPIIASISSGVVSIWAQNQVENWSAFAPDFKELDENVEYEERESEFDIEDEDKSEPEQTGADAAEDEEVDVTSVDPIAAFCSSDEELEDSKALLYLPIAPEVEDPEENPYGPPPDAVQTSLMDEGASSEKKRQSSADGSQPPKKKPKTTNIELQGVPNDEVHPLLGVKGDGKSKKKQAGRPKGSKGKEKDSPFKPKLYKGDRGLPLEGSAKGKVQAELSQPLTAGGAISELL","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":538,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q15291","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Form
Liquid
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RbBP5 also known as Retinoblastoma binding protein 5 acts as a core component of the Set1/COMPASS complex which plays a role in histone methylation. The RbBP5 protein weighs approximately 58 kDa and it is found throughout various tissues with notable expression in areas engaged in active transcription. This widespread presence suggests its substantial impact on chromatin modification processes.
Biological function summary

RbBP5 functions to assist in the regulation of gene transcription. It is an integral part of the Set1/COMPASS complex which is responsible for the tri-methylation of histone H3 at lysine 4 (H3K4me3) a modification associated with transcriptionally active genes. By participating in this complex RbBP5 contributes to the establishment and maintenance of euchromatin regions thereby allowing transcription factors and other proteins involved in gene expression to access DNA more easily.

Pathways

RbBP5 plays a role in chromatin remodeling and transcription regulation pathways. Its involvement in the H3K4me3 modification process places it in the TRX pathway influencing various gene expression patterns. RbBP5 interacts with other components of the Set1/COMPASS complex including ASH2L and WDR5 which collaboratively modulate the levels of histone methylation and ultimately affect gene transcription activities.

RbBP5 has connections to cancer and developmental disorders due to its role in gene expression regulation. Abnormal RbBP5 activity can disrupt proper H3K4 methylation which may lead to either the inappropriate activation or silencing of genes implicating it in oncogenic processes. In cancer progression interactions between RbBP5 and other components like the MLL protein (mixed-lineage leukemia) become significant as mutations or dysregulation within this complex contribute to the pathogenesis of leukemia and other malignancies.
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General info

Function

In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). Does not affect ES cell self-renewal (By similarity). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed : 19131338). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 19556245). In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

Subcellular localisation

Nucleus

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Product protocols

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Target data

In embryonic stem (ES) cells, plays a crucial role in the differentiation potential, particularly along the neural lineage, regulating gene induction and H3 'Lys-4' methylation at key developmental loci, including that mediated by retinoic acid (By similarity). Does not affect ES cell self-renewal (By similarity). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed : 19131338). As part of the MLL1/MLL complex, involved in mono-, di- and trimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). Histone H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 19556245). In association with ASH2L and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).
See full target information RBBP5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 50:4732-4754 PubMed35420136

2022

Microarray screening reveals two non-conventional SUMO-binding modules linked to DNA repair by non-homologous end-joining.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Jose Cabello-Lobato,Matthew Jenner,Metztli Cisneros-Aguirre,Kira Brüninghoff,Zac Sandy,Isabelle C da Costa,Thomas A Jowitt,Christian M Loch,Stephen P Jackson,Qian Wu,Henning D Mootz,Jeremy M Stark,Matthew J Cliff,Christine K Schmidt
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