Recombinant Human RCC1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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- Molecular & cellular proteomics : MCP 17:2018-20332018Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDomenico Fasci,Hugo van Ingen,Richard A Scheltema,Albert J R HeckPubMed 30021884
- BMC plant biology 16:422016Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelanie Binkert et. al.PubMed 26864020
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab90625 can be used as a WB positive control in conjunction with Anti-RCC1 antibody ab70749. |
Target data
Function
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:11336674, PubMed:17435751, PubMed:1944575, PubMed:20668449, PubMed:22215983, PubMed:29042532). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).
Alternative names
CHC1, RCC1, Regulator of chromosome condensation, Cell cycle regulatory protein, Chromosome condensation protein 1
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Recombinant Human RCC1 protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P18754-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.6
Constituents: 0.476% HEPES, 0.29% Sodium chloride, 0.0308% 1,4-Dithioerythritol
Specifications
- Form
- Liquid
General info
- Function
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed:11336674, PubMed:17435751, PubMed:1944575, PubMed:20668449, PubMed:22215983, PubMed:29042532). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed:12194828, PubMed:17435751, PubMed:22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed:22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed:3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed:17435751, PubMed:18762580).
- Post-translational modifications
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Regulator of Chromosome Condensation 1 (RCC1) also known as Ran guanine nucleotide exchange factor is a highly conserved protein. With a molecular mass of approximately 45 kDa RCC1 localizes mainly in the nucleus. This protein functions as a guanine nucleotide exchange factor (GEF) for the small GTPase Ran facilitating the conversion of RanGDP to RanGTP. RCC1 is abundant in cells showing expression in various tissue types reflecting its mechanical role in nuclear transport.
- Biological function summary
The protein RCC1 acts as an important player in the regulation of nucleocytoplasmic transport mitosis and other cellular processes. It forms a complex with chromatin and its substrate Ran particularly binding to DNA in a sequence-specific manner. RCC1's activity modifies the Ran gradient across the nuclear envelope which is essential for directing the import and export of proteins and RNA molecules. This gradient is also vital during mitosis for proper chromosomal segregation.
- Pathways
RCC1 integrates into the Ran pathway a mechanism significant for cell cycle progression and nucleocytoplasmic transport. Within this pathway RCC1 influences the Ran cycle in partnership with proteins such as RanBP1 and RanGAP. It also plays a part in mitotic spindle assembly through its interactions with spindle assembly factors interlinking with microtubule dynamics. RCC1's function in these pathways highlights its critical involvement in maintaining cellular division and transport activities.
- Associated diseases and disorders
Defects in RCC1 expression or function have connections to tumorigenesis and certain neurological disorders. Aberrations in the Ran signaling pathway mediated by RCC1 can disturb genomic stability linking it to cancer progression. Additionally improper regulation of RCC1 might implicate its association with neurodegenerative conditions where proteins like RanGAP1 and importin beta are also involved. Understanding these relationships may lead to potential therapeutic targets for treating such diseases.
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2 product images
Western blot - Recombinant Human RCC1 protein (ab90625)
All lanes: Western blot - Anti-RCC1 antibody (Anti-RCC1 antibody ab70749) at 1/2000 dilution
Lane 1: Western blot - Recombinant Human RCC1 protein (ab90625) at 0.01 µg
Lane 2: Western blot - Recombinant Human RCC1 protein (ab90625) at 0.1 µg
SecondaryAll lanes: Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8min
Western blot - Recombinant Human RCC1 protein (ab90625)
All lanes: Western blot - Anti-RCC1 antibody (Anti-RCC1 antibody ab70749) at 1/2000 dilution
Lane 1: Western blot - Recombinant Human RCC1 protein (ab90625) at 0.01 µg
Lane 2: Western blot - Recombinant Human RCC1 protein (ab90625) at 0.1 µg
SecondaryAll lanes: Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8min
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