Recombinant Human RCC1 protein

Specifications

Form

Liquid

General info

Function

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed : 11336674, PubMed : 17435751, PubMed : 1944575, PubMed : 20668449, PubMed : 22215983, PubMed : 29042532). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed : 12194828, PubMed : 17435751, PubMed : 22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed : 22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed : 3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed : 17435751, PubMed : 18762580).

Post-translational modifications

N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.

Subcellular localisation

Nucleus