Recombinant Human RCC1 Protein (His Tag)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human RCC1 Protein (His Tag) is a Human Full Length protein expressed in E. coli cells using an N-terminal His tag with >90% purity, and is suitable for SDS-PAGE. The predicted molecular weight of ab325766 protein is ~50 kDa.
View Alternative Names
CHC1, RCC1, Regulator of chromosome condensation, Cell cycle regulatory protein, Chromosome condensation protein 1
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human RCC1 Protein (His Tag) (AB325766)
The purity of Recombinant Human RCC1 Protein (ab325766) was determined to be >90% by densitometry.
Oberved MW : 50 kDa.
Calculated MW : 49 kDa.
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Specifications
Form
Liquid
General info
Function
Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis (PubMed : 11336674, PubMed : 17435751, PubMed : 1944575, PubMed : 20668449, PubMed : 22215983, PubMed : 29042532). Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis (PubMed : 12194828, PubMed : 17435751, PubMed : 22215983). Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import (PubMed : 22215983). Involved in the regulation of onset of chromosome condensation in the S phase (PubMed : 3678831). Binds both to the nucleosomes and double-stranded DNA (PubMed : 17435751, PubMed : 18762580).
Post-translational modifications
N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Subcellular localisation
Nucleus
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com